Comparative Transcriptome Analysis of Three Oil Palm Fruit and Seed Tissues That Differ in Oil Content and Fatty Acid Composition

Dussert, Stéphane; Guerin, Chloé; Andersson, Mariette; Joët, Thierry; Tranbarger, Timothy John; Pizot, Maxime; Sarah, Gautier; Omoré, Alphonse; Durand‐Gasselin, Tristan; Morcillo, Fabienne

doi:10.1104/pp.113.220525

Cited by 197 publications

(230 citation statements)

References 85 publications

(141 reference statements)

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“…S3), suggesting that this approach enhances TAG accumulation in all vegetative tissues of B. distachyon. These results are consistent with previous studies conducted in dicotyledonous plants (Cernac and Benning, 2004;Sanjaya et al, 2011;Dussert et al, 2013;Kelly et al, 2013;Vanhercke et al, 2013Vanhercke et al, , 2014Grimberg et al, 2015) and confirm that BdWRI1 has a conserved function in regulating FA and, indirectly, TAG biosynthesis and that ectopic production of WRI1 serves as a promising tool for enhancing the energy density in vegetative tissues of plants.…”

Section: Wri1 Has a Conserved Function In Fa Biosynthesis And Tag Accsupporting

confidence: 92%

“…Despite the fact that WRI1 shows conserved function in FA biosynthesis and oil production in planta, different expression patterns of WRI1 orthologs or additional phenotypes of the respective overexpression or mutant lines have been reported in many species. AtWRI1 is specifically expressed in siliques, whereas oil palm EgWRI1 is highly expressed in mesocarp (Tranbarger et al, 2011;Dussert et al, 2013). The Arabidopsis wri1 mutant does not show any obvious phenotype in nonseed tissues (Focks and Benning, 1998), whereas silencing of WRI1 expression in cotton (Gossypium spp.)…”

Section: Functional Divergence Of Lipid Metabolism Between Monocots Amentioning

confidence: 99%

“…WRI1 orthologs from maize (Zea mays), rape (Brassica napus), and oil palm (Elaeis guineensis) have been shown to be involved in TAG production in embryos or fruit mesocarps, and their overexpression leads to increased seed oil content (Liu et al, 2010;Shen et al, 2010;Pouvreau et al, 2011;Ma et al, 2013;van Erp et al, 2014). Ectopic expression of WRI1 has also been used to stimulate oil production in nonseed tissues in both Arabidopsis and tobacco (Nicotiana tabacum; Cernac and Benning, 2004;Sanjaya et al, 2011;Dussert et al, 2013;Kelly et al, 2013;Vanhercke et al, 2014;Grimberg et al, 2015;Ma et al, 2015).…”

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Ectopic expression of WRI1 affects fatty acid homeostasis in Brachypodium distachyon vegetative tissues

et al. 2015

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Triacylglycerol (TAG) is a storage lipid used for food purposes and as a renewable feedstock for biodiesel production. WRINKLED1 (WRI1) is a transcription factor that governs fatty acid (FA) synthesis and, indirectly, TAG accumulation in oil-storing plant tissues, and its ectopic expression has led to TAG accumulation in vegetative tissues of different dicotyledonous plants. The ectopic expression of BdWRI1 in the grass Brachypodium distachyon induced the transcription of predicted genes involved in glycolysis and FA biosynthesis, and TAG content was increased up to 32.5-fold in 8-week-old leaf blades. However, the ectopic expression of BdWRI1 also caused cell death in leaves, which has not been observed previously in dicotyledonous plants such as Arabidopsis (Arabidopsis thaliana). Lipid analysis indicated that the free FA content was 2-fold elevated in BdWRI1-expressing leaf blades of B. distachyon. The transcription of predicted genes involved in b-oxidation was induced. In addition, linoleic FA treatment caused cell death in B. distachyon leaf blades, an effect that was reversed by the addition of the FA biosynthesis inhibitor cerulenin. Taken together, ectopic expression of BdWRI1 in B. distachyon enhances FA biosynthesis and TAG accumulation in leaves, as expected, but also leads to increased free FA content, which has cytotoxic effects leading to cell death. Thus, while WRI appears to ubiquitously affect FA biosynthesis and TAG accumulation in diverse plants, its ectopic expression can lead to undesired side effects depending on the context of the specific lipid metabolism of the respective plant species.

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Section: Wri1 Has a Conserved Function In Fa Biosynthesis And Tag Accsupporting

confidence: 92%

Section: Functional Divergence Of Lipid Metabolism Between Monocots Amentioning

confidence: 99%

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Ectopic expression of WRI1 affects fatty acid homeostasis in Brachypodium distachyon vegetative tissues

et al. 2015

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“…In many ways transcriptome sequences can be much more useful than genome sequences because they only include the particular fraction of the tens of thousands of genes that are expressed under specific conditions. In the case of oil palm, the analysis of the fruit transcriptome during oil accumulation is already proving very useful in identifying key genes that may regulate this vital process (Bourgis et al, 2011;Dussert et al, 2013;Tranbarger et al, 2011).…”

Section: Beyond the Genome: Other "Omic" Technologiesmentioning

confidence: 99%

Using modern plant breeding to improve the nutritional and technological qualities of oil crops

Murphy

2014

OCL

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-The last few decades have seen huge advances in our understanding of plant biology and in the development of new technologies for the manipulation of crop plants. The application of relatively straightforward breeding and selection methods made possible the "Green Revolution" of the 1960s and 1970s that effectively doubled or trebled cereal production in much of the world and averted mass famine in Asia. During the 2000s, much attention has been focused on genomic approaches to plant breeding with the deployment of a new generation of technologies, such as marker-assisted selection, next-generation sequencing, transgenesis (genetic engineering or GM) and automatic mutagenesis/selection (TILLING, TargetIng Local Lesions IN Genomes). These methods are now being applied to a wide range of crops and have particularly good potential for oil crop improvement in terms of both overall food and non-food yield and nutritional and technical quality of the oils. Key targets include increasing overall oil yield and stability on a per seed or per fruit basis and very high oleic acid content in seed and fruit oils for both premium edible and oleochemical applications. Other more specialised targets include oils enriched in nutritionally desirable "fish oil"-like fatty acids, especially very long chain ω-3 acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), or increased levels of lipidic vitamins such as carotenoids, tocopherols and tocotrienes. Progress in producing such oils in commercial crops has been good in recent years with several varieties being released or at advanced stages of development.Résumé -Utilisation de la sélection variétale moderne pour améliorer les qualités nutritionnelles et technologiques des cultures oléagineuses. Les dernières décennies ont connu de grandes avancées dans la compréhension de la biologie des plantes et dans le développement de nouvelles technologies de manipulation des semences. L'application de méthodes de croisements variétal et les méthodes de sélection ont rendu possible la révolution verte des années 1960 et 1970 qui ont efficacement doublé voire triplé la production céréalière dans une grande partie du monde et évité des famines de masse en Asie. Durant les années 2000, davantage d'attention a été portée à des approches génomiques en sélection des plantes avec le déploiement d'une nouvelle génération de technologies, comme la sélection assistée par des marqueurs, le séquençage de la génération suivante, la transgénèse (ingénierie génétique ou OGM) et la mutagé-nèse/sélection automatique (ILLI NG, TargetIng Local Lesions IN Genomes). Ces méthodes sont désormais appliquées à un large éventail de semences et offrent, pour les oléagineux, un potentiel particulièrement intéressant à la fois en termes de rendement alimentaire et non-alimentaire, en qualité nutritionnelle et technique des huiles. Les objectifs clés incluent une amélioration du rendement global en huile et de la stabilité par graine ou par fruit, et un contenu très élevé en acide oléique da...

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“…Some studies have focused on the non-seed, oil accumulating mesocarp tissue of food crops like avocado, oil palm and olive [6][7][8] . However, for the development of a more sustainable biofuel crop species it is imperative that it does not compete with or displace the land for food production.…”

Section: Research Highlightmentioning

confidence: 99%

Oil accumulation in non-seed tissue: transcriptome analysis of Chinese tallow

Divi

2016

Genes Transl Bioinform

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Chinese tallow (Triadica sebifera) is an oil-seed producing tree with a great potential as a biofuel crop and does not compete with food crops for land. The fruits are unique in that they contain two kinds of lipid, a highly un-saturated oil and a saturated fat separated in the seed and an external tallow layer respectively. However, until recently it was unclear whether the tallow is a non-seed tissue or an external fat deposition. We have addressed this important question by scanning electron microscopy to show that it is a cellular tissue and provided biochemical evidence for an active storage oil or triacylglycerol (TAG) biosynthetic machinery in the non-seed tallow tissue. We also showed that tallow tissue accumulates more TAG and has a different fatty acid profile compared to the seed TAG. As a sequence resource to aid the development of this oil crop, we generated a transcriptome resource by sequencing three component fruit tissues of tallow, seed and fruit coat. This enabled the reconstruction of lipid biosynthesis pathway in this potential biofuel crop. The transcriptome resource has thus far allowed identification of key differences in oil synthesis and storage mechanisms between the seed and tallow tissues. This non-seed oil accumulating tissue can be used as model system to gain further knowledge of such tissues to facilitate efficient utilization of non-seed biomass for oil production.

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Comparative Transcriptome Analysis of Three Oil Palm Fruit and Seed Tissues That Differ in Oil Content and Fatty Acid Composition

Cited by 197 publications

References 85 publications

Ectopic expression of WRI1 affects fatty acid homeostasis in Brachypodium distachyon vegetative tissues

Ectopic expression of WRI1 affects fatty acid homeostasis in Brachypodium distachyon vegetative tissues

Using modern plant breeding to improve the nutritional and technological qualities of oil crops

Oil accumulation in non-seed tissue: transcriptome analysis of Chinese tallow

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