Comparative transcriptome analysis reveals multiple functions for Mhy1p in lipid biosynthesis in the oleaginous yeast Yarrowia lipolytica

Wang, Guangyuan; Li, Delong; Miao, Zhengang; Zhang, Shanshan; Liang, Wenxing; Liu, Lin

doi:10.1016/j.bbalip.2017.10.003

Cited by 31 publications

(22 citation statements)

References 59 publications

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“…Both strains showed a similar growth profile (Fig 2A). We observed that the MHY1-deletion strain accumulated somewhat more lipid than the wild type in both nitrogen sources tested (Fig 2B), in line with previous findings [25,26], further strengthening the beneficial traits of the MHY1-deletion. We also studied the fatty acid composition of the two strains, which did not show any significant changes (Fig 2C, detailed in supplement Table 1).…”

Section: Effect Of Mhy1-deletion On Lipid Accumulation and Compositionsupporting

confidence: 90%

“…Finally, we tested the deletion of the MHY1 gene, which has a C2H2-zinc finger, DNAbinding domain on the C-terminal. Deletion of MHY1 has been shown to abolish hyphae formation [11,[24][25][26], while overexpression increased hyphae formation [11]. In our hands, the MHY1-deletion (OKYL029) was the only strain which abolished hyphae formation under all tested conditions (Fig 1).…”

Section: Plos Onementioning

confidence: 56%

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Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Konzock¹,

Norbeck²

2020

PLoS ONE

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There is a need for development of sustainable production processes for production of fats/ oils and lipid derived chemicals. The dimorphic oleaginous yeast Yarrowia lipolytica is a promising organism for conversion of biomass hydrolysate to lipids, but in many such processes hyphae formation will be problematic. We have therefore constructed and compared the performance of strains carrying deletions in several published gene targets suggested to abolish hyphae formation (MHY1, HOY1 and CLA4). The MHY1-deletion was the only of the tested strains which did not exhibit hyphae formation under any of the conditions tested. The MHY1-deletion also had a weak positive effect on lipid accumulation without affecting the total fatty acid composition, irrespective of the nitrogen source used. MHY1 has been suggested to constitute a functional homolog of the stress responsive transcription factors MSN2/4 in Saccharomyces cerevisiae, the deletion of which are highly stress sensitive. However, the deletion of MHY1 displayed only minor difference on survival of a range of acute or long term stress and starvation conditions. We conclude that the deletion of MHY1 in Y.lipolytica is a reliable way of abolishing hyphae formation with few detectable negative side effects regarding growth, stress tolerance and lipid accumulation and composition.

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Section: Effect Of Mhy1-deletion On Lipid Accumulation and Compositionsupporting

confidence: 90%

Section: Plos Onementioning

confidence: 56%

Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Konzock¹,

Norbeck²

2020

PLoS ONE

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“…An RNA-seq library was constructed using the NEBNext ® Ultra ™ RNA Library Prep kit for Illumina ® (New England Biolabs, Ipswich, MA, USA) according to the manufacturer's instructions. RNA library quality confirmation and RNA sequencing were carried out according to the process described by Wang et al (2018a).…”

Section: Rna Sequencing and Rna-seq Data Analysismentioning

confidence: 99%

Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

et al. 2020

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In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P. pastoris GS115m/LacB was subsequently constructed via introducing a β-galactosidase gene, LacB, under the control of rhamnose-inducible P LRA3 into P. pastoris GS115m. P. pastoris GS115m/LacB greatly improved recombinant protein production relative to the parental strain (P. pastoris GS115/LacB). In the present study, transcriptomes of P. pastoris GS115m/LacB and P. pastoris GS115/LacB grown in YPR medium were analyzed. P. pastoris GS115m/LacB was found to suffer from the mild carbon source starvation. To attenuate the starvation stress, P. pastoris GS115m/LacB attempted to enhance rhamnose metabolism by elevating the transcription levels of rhamnose-utilization genes LRA1-3 and RhaR. The transcription level of LacB under the control of P LRA3 thereby increased, resulting in the improved production of recombinant protein in P. pastoris GS115m/LacB. It was also revealed that P. pastoris GS115m/LacB cells coped with carbon starvation mostly via autophagy.

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“…In subsequent work, the same researchers showed that leucine biosynthesis was particularly downregulated when the yeast was grown under nitrogen-limited conditions, concomitantly with lipid accumulation ( Kerkhoven et al, 2017 ). A recent study looked at transcriptional changes in Y. lipolytica during lipid biosynthesis in strain carrying a MHY1 gene inactivation ( Wang et al, 2018 ); MHY1 encodes a C 2 H 2 -type zinc finger protein. They found that nearly 25% of annotated Y. lipolytica genes were expressed at significantly different levels, suggesting Mhyp plays a crucial regulatory role in various biological processes, including lipid and amino acid metabolism (again underscoring the interaction between these pathways).…”

Section: Computational Toolsmentioning

confidence: 99%

Synthetic biology tools for engineering Yarrowia lipolytica

Larroude

Rossignol

Nicaud

et al. 2018

Biotechnology Advances

135

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The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools.

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Comparative transcriptome analysis reveals multiple functions for Mhy1p in lipid biosynthesis in the oleaginous yeast Yarrowia lipolytica

Cited by 31 publications

References 59 publications

Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

Synthetic biology tools for engineering Yarrowia lipolytica

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