Comparative Transcriptome Analysis Reveals Related Regulatory Mechanisms of Androgenic Gland in<i>Eriocheir sinensis</i>

Fu, Chunpeng; Zeng, Qifan; Li, Fajun; Wang, Huicui; Sun, Jian

doi:10.1155/2017/4956216

Cited by 7 publications

(3 citation statements)

References 63 publications

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“…Insulin-like receptor (IR) functions as the pivotal member of insulin family signaling pathway and directs the male sexual differentiation in mammals [46]. Earlier evidence has shown that IR interacts with the insulin-like androgenic gland hormone (IAG) to regulate sex differentiation and spermatogenesis in crustaceans [13,15,46,47]. In our study, IR has shown higher expression in ovaries than in testes.…”

Section: Discussionsupporting

confidence: 52%

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Jiang

Yuan

Qingqing

et al. 2019

Genes

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The giant freshwater prawn (Macrobrachium rosenbergii) exhibits sex dimorphism between the male and female individuals. To date, the molecular mechanism governing gonadal development was unclear, and limited data were available on the gonad transcriptome of M. rosenbergii. Here, we conducted comprehensive gonadal transcriptomic analysis of female (ZW), super female (WW), and male (ZZ) M. rosenbergii for gene discovery. A total of 70.33 gigabases (Gb) of sequences were generated. There were 115,338 unigenes assembled with a mean size of 1196 base pair (bp) and N50 of 2195 bp. Alignment against the National Center for Biotechnology Information (NCBI) non-redundant nucleotide/protein sequence database (NR and NT), the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, SwissProt database, Protein family (Pfam), Gene ontology (GO), and the eukaryotic orthologous group (KOG) database, 36,282 unigenes were annotated at least in one database. Comparative transcriptome analysis observed that 10,641, 16,903, and 3393 genes were significantly differentially expressed in ZW vs. ZZ, WW vs. ZZ, and WW vs. ZW samples, respectively. Enrichment analysis of differentially expressed genes (DEGs) resulted in 268, 153, and 42 significantly enriched GO terms, respectively, and a total of 56 significantly enriched KEGG pathways. Additionally, 23 putative sex-related genes, including Gtsf1, IR, HSP21, MRPINK, Mrr, and other potentially promising candidate genes were identified. Moreover, 56,241 simple sequence repeats (SSRs) were identified. Our findings provide a valuable archive for further functional analyses of sex-related genes and future discoveries of underlying molecular mechanisms of gonadal development and sex determination. delicious flesh and high value nutrition. Like other crustaceans, M. rosenbergii displays sexual dimorphic growth pattern: The male individuals grow much larger and faster than the females [1,2]. Thus, based on the economic impact, developing the monosex population culture as the efficient approach to boost production has received great attention from researchers in M. rosenbergii. Nevertheless, the premise of monosex culture is to understand the molecular mechanism of sex determination, as well as to unravel sex-related genes.Studies on sex determination have proved that many macruran species exhibit the ZW/ZZ sex determination system [3][4][5]. In M. rosenbergii, sex reversal experiments proposed that the female prawns bear the ZW sex chromosomes, and the male individuals bear the ZZ chromosomes [1,6,7]. Subsequently, isolation of sex-specific markers further deepened the insight of the ZW/ZZ sex determination mode [8,9]. Recently, Ma et al. have confirmed that M. rosenbergii has the ZW/ZZ sex determination system based on the bacterial artificial chromosome (BAC) library, and nine genes were unraveled to be the key sex-linked genes by PacBio sequencing. Amongst, three genes, including zinc knuckle domain (ZKD), reverse transcriptase, and ANCDUO, protein homologs were known genes without characteri...

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Section: Discussionsupporting

confidence: 52%

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Jiang

Yuan

Qingqing

et al. 2019

Genes

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“…From the couple of stages examined in M. rosenbergii (Sharabi et al, 2016) and S. verreauxi , the same goes for decapods. The only differential expression reported regards an unspecified IR paralog in Eriocheir sinensis: it is up-regulated during the secretion phase of the androgenic gland, compared to the proliferation phase (Fu et al, 2017). This functioning of the gland is related to the exacerbation of sex characters as males ready for mating, but this does not occur in isopods.…”

Section: Targeted Silencing Of Av-ir1 and Av-ir2 By Rnaimentioning

confidence: 97%

Feminising Wolbachia disrupt Armadillidium vulgare insulin‐like signalling pathway

Herran

Houdelet

Raimond

et al. 2021

Cellular Microbiology

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The endosymbiont Wolbachia feminises male isopods by making them refractory to the insulin-like masculinising hormone, which shunts the autocrine development of the androgenic glands. It was, therefore, proposed that Wolbachia silences the IR receptors, either by preventing their expression or by inactivating them. We describe here the two IR paralogs of Armadillidium vulgare. They displayed a conventional structure and belonged to a family widespread among isopods. Av-IR1 displayed an ubiquist expression, whereas the expression of Av-IR2 was restricted to the gonads. Both were constitutively expressed in males and females and throughout development. However, upon silencing, altered gland physiology and gene expression therein suggested antagonistic roles for Av-IR1 (androinhibiting) and Av-IR2 (androstimulating). They may function in tandem with regulating neurohormones, as a conditional platform that conveys insulin signalling. Wolbachia infection did not alter their expression patterns: leaving the IRs unscathed, the bacteria would suppress the secretion of the neurohormones, thus inducing body-wide IR deactivation and feminisation. Adult males injected with Wolbachia acquired an intersexed physiology. Their phenotypes and gene expressions mirrored the silencing of Av-IR1 only, suggesting that imperfect feminisation stems from a flawed invasion of the androstimulating centre, whereas in fully feminised males invasion would be complete in early juveniles. Take Away• Two antagonistic Insulin Receptors were characterised in Armadillidium vulgare.• The IRs were involved in androstimulating and androinhibiting functions.• Wolbachia-induced feminisation did not prevent the expression of the IRs.• Imperfectly feminised intersexes phenocopied the silencing of Av-IR1 only.• Wolbachia would deactivate the IRs by suppressing neurosecretory co-factors.

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“…In addition, with the development of sequencing technology and molecular biology, more sex-related genes have been successfully mined, such as Vasa, Foxl2, Vitellogenin (Vg), Ecdysone Receptor (EcR), Sry, Ftz-f1, etc. [88]- [95], however, there is still a lack of research on the functional mechanisms of genes in sex determination, differentiation and gonadal development.…”

Section: Other Sex Determination and Differentiation-related Genesmentioning

confidence: 99%

A Review of Sexual Determination and Differentiation in Crustacean

Li¹

2022

JBM

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The prerequisite for monosex culture is to elucidate the molecular mechanism of sex determination and differentiation in crustaceans, as well as to explore the key genes that play a role in this process. Monosex culture technology based on crustacean economic species is of great importance in terms of genetic breeding and economic benefits of aquaculture. As a result, study into the mechanisms of sex determination and differentiation in crustaceans not only contributes to the current absence of basic theories of crustacean sexual mechanism, but also encourages technical innovation in aquaculture to increase overall economic efficiency. This study synthesizes and evaluates available research on sex determination and differentiation in crustaceans, and then provides recommendations for future research objectives and priorities in the field.

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Comparative Transcriptome Analysis Reveals Related Regulatory Mechanisms of Androgenic Gland inEriocheir sinensis

Cited by 7 publications

References 63 publications

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Feminising Wolbachia disrupt Armadillidium vulgare insulin‐like signalling pathway

A Review of Sexual Determination and Differentiation in Crustacean

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