Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin

Abstract

Samia cynthia ricini (Lepidoptera: Saturniidae) is an important commercial silk-producing insect; however, in contrast to the silkworm, mulberry leaves are toxic to this insect because the leaves contain the component 1-deoxynojirimycin (DNJ). A transcriptomic analysis of eri-silkworm haemolymph was conducted to examine the genes related to different metabolic pathways and to elucidate the molecular mechanism underlying eri-silkworm haemolymph responses to DNJ. Eight hundred sixty-five differentially expressed genes (DEGs) were identified, among which 577 DEGs were up-regulated and 288 DEGs were down-regulated in the 2% DNJ group compared to control (ddH2O) after 12h. Based on the results of the functional analysis, these DEGs were associated with ribosomes, glycolysis, N-glycan biosynthesis, and oxidative phosphorylation. In particular, according to the KEGG analysis, 138 DEGs were involved in energy metabolism, glycometabolism and lipid metabolism, and the changes in the expression of nine DEGs were confirmed by reverse transcription quantitative PCR (RT-qPCR). Thus, DNJ induced significant metabolic alterations in eri-silkworm haemolymph. These results will lay the foundation for research into the toxic effects of DNJ on eri-silkworm as a model and provide a reference for the exploitation of new drugs in humans.

1H NMR‐based metabonomic evaluation of the pesticides camptothecin and matrine against larvae of Spodoptera litura

“…Previously, the differential genes of key enzymes involved in the energy metabolism were gathered from transcriptional profiling. 27 Combined with the metabolomic results, six genes were confirmed by RT-qPCR. Total RNA (1 μg) was reversely transcribed following the protocol of PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time; Takara, China) and the qPCR amplification was carried out using TB Green® Fast qPCR Mix (Takara) in the CFX96 Touch™ System (Bio-Rad, USA).The relative transcriptional analysis of target genes was carried out according to the 2 −44Ct method.…”

“…Previously, the differential genes of key enzymes involved in the energy metabolism were gathered from transcriptional profiling 27 . Combined with the metabolomic results, six genes were confirmed by RT‐qPCR.…”

Integrated transcriptome sequencing and RNA interference reveals molecular changes in Diaphorina citri after exposure to validamycin

“…The hierarchical clustering analysis suggested that most of the genes were up-regulated form 12 to 24 h after the validamycin treatment and were then down-regulated by 36 h. In Stenocoris furcifera, a comparative transcriptome analysis under three different insecticide-treatment conditions revealed that genes related to GST, P450s, ABC transporters and Hsps were up-regulated (Zhou et al, 2018). Zhang et al (2018) revealed that 577 DEGs were up-regulated and 288 DEGs were downregulated in Samia cynthia ricini after being treated with 1-deoxynojirimycin, and these genes were involved mainly in energy metabolism, glycometabolism and lipid metabolism (Zhang et al, 2018). Thus, the host might up-regulate the expression levels of these genes to defend against infiltration by exogenous entities.…”

Identification of FerLCH, isolation of ferritin and functional analysis related to interaction with pathogens in Eri‐silkworm, Samia cynthia ricini

“…Transcriptome sequencing was performed on fifth‐instar larval hemolymph of S. c. ricini using the Illumina sequencing method (Beijing Novogene Bioinformatics Technology, Beijing, China) to obtain the S. c. ricini transcriptome database (Zhang et al, 2018). ScFerLCH was identified from the data set by TBLASTN.…”