Comparative transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis in Eucommia ulmoides Oliv.

Ye, Jing; Han, Wenjing; Deng, Peng; Jiang, Yangyang; Liu, Minhao; Li, Long; Li, Zhouqi

doi:10.1007/s00468-019-01865-y

Cited by 20 publications

(14 citation statements)

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“…Eucommia ulmoides ( E. ulmoides ) Oliv. (Family: Eucommiaceae) is a native tree in China ( Wuyun et al, 2018 ), which has been used for centuries in clinical treatment according to ancient Chinese medicine books such as the Shen-nong’s Classic of Materia Medica and Compendium ( Ding and Wang, 2015 ; Ye et al, 2019 ). The bark, roots, and leaves of E. ulmoides have been reported as a good source of highly potent medicinal compounds ( Xing et al, 2019 ), e.g.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Sayed

Siems²,

Schmitz‐Linneweber

et al. 2021

Front. Pharmacol.

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To uncover potential anti-aging capacities of Traditional Chinese Medicine (TCM), the nematode Caenorhabditis elegans was used to investigate the effects of Eucommia ulmoides and Cuscuta chinensis extracts, selected by screening seven TCM extracts, on different healthspan parameters. Nematodes exposed to E. ulmoides and C. chinensis extracts, starting at the young adult stage, exhibited prolonged lifespan and increased survival after heat stress as well as upon exposure to the pathogenic bacterium Photorhabdus luminescens, whereby the survival benefits were monitored after stress initiation at different adult stages. However, only C. chinensis had the ability to enhance physical fitness: the swimming behavior and the pharyngeal pumping rate of C. elegans were improved at day 7 and especially at day 12 of adulthood. Finally, monitoring the red fluorescence of aged worms revealed that only C. chinensis extracts caused suppression of intestinal autofluorescence, a known marker of aging. The results underline the different modes of action of the tested plants extracts. E. ulmoides improved specifically the physiological fitness by increasing the survival probability of C. elegans after stress, while C. chinensis seems to be an overall healthspan enhancer, reflected in the suppressed autofluorescence, with beneficial effects on physical as well as physiological fitness. The C. chinensis effects may be hormetic: this is supported by increased gene expression of hsp-16.1 and by trend, also of hsp-12.6.

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Section: Introductionmentioning

confidence: 99%

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Sayed

Siems²,

Schmitz‐Linneweber

et al. 2021

Front. Pharmacol.

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“…These studies showed that the expression of HCT consisted of a multigene regulatory process in perennial forest trees. In addition, the biosynthetic pathway of CGA also involves MYB , ERF , WRKY , and other transcription factor gene families [ 14 ]. Some of their members, including MYB83 , MYB114 , ERF35 , ERF109 and WRKY19 , were also identified in our study ( Table S4 and Data S1 ).…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have found that several catalytic enzymes control CGA biosynthesis. For example, HQT (hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase), a crucial enzyme involved in downstream regulation of the CGA biosynthetic pathway, has been confirmed as the rate-limiting step in many plant species [ 14 ]. In the mulberry tree, HCT4 (hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyl transferase 4) increases the CGA content by promoting the biosynthesis of p -coumaroylshikimic acid and p -coumaroylquinic acid after a frost [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Architecture Underlying the Metabolites of Chlorogenic Acid Biosynthesis in Populus tomentosa

Yao

et al. 2021

IJMS

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Chlorogenic acid (CGA) plays a crucial role in defense response, immune regulation, and the response to abiotic stress in plants. However, the genetic regulatory network of CGA biosynthesis pathways in perennial plants remains unclear. Here, we investigated the genetic architecture for CGA biosynthesis using a metabolite-based genome-wide association study (mGWAS) and expression quantitative trait nucleotide (eQTN) mapping in a population of 300 accessions of Populus tomentosa. In total, we investigated 204 SNPs which were significantly associated with 11 metabolic traits, corresponding to 206 genes, and were mainly involved in metabolism and cell growth processes of P. tomentosa. We identified 874 eQTNs representing 1066 genes, in which the expression and interaction of causal genes affected phenotypic variation. Of these, 102 genes showed significant signatures of selection in three geographical populations, which provided insights into the adaptation of CGA biosynthesis to the local environment. Finally, we constructed a genetic network of six causal genes that coordinately regulate CGA biosynthesis, revealing the multiple regulatory patterns affecting CGA accumulation in P. tomentosa. Our study provides a multiomics strategy for understanding the genetic basis underlying the natural variation in the CGA biosynthetic metabolites of Populus, which will enhance the genetic development of abiotic-resistance varieties in forest trees.

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“…Zhang et al (2018) utilized GWAS combined with eQTL analyses to find a significant correlation between PtHCT2 and CGA in poplar trees. Meanwhile, it was reported some transcription factors (MYB, ZIP, WRKY and ERF) are involved in the regulation of CGA biosynthesis (Ye et al 2019). In Lonicera japonica, LjbZIP8 was found as a transcription inhibitor that can specifically bind to the G-box element of LjPAL2 5 '-UTR, and reduce the content of CGA when it is overexpressed in tobacco (Zha et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analyses of Chlorogenic Acid Biosynthesis Pathways in Diploid and Triploid Camellia sinensis

Shen¹

2021

IJAB

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Chlorogenic acid (CGA), as a kind of depside in plants, has a variety of beneficial effects on human health, which also plays an important role in helping plants resist a variety of stresses. Therefore, the biosynthetic pathway of CGA has been studied in many plants, however, the synthesis of CGA has not been well elucidated in Camellia sinensis. In our research, different CGA levels were detected between triploid tea variety „Qianfu 4‟ and diplont tea variety „Qianmei 419‟ using HPLC and the CGA content in triploid Camellia sinensis was greater than that in diploid Camellia sinensis. Transcriptome sequencing for diploid and triploid Camellia sinensis was employed to explore genes associated with CGA biosynthesis. Finally, 154,097 unigenes were obtained in total, of which 891 may be related to the biosynthesis of CGA. Furthermore, differentially expressed genes (DEGs) were screened between diploid and triploid Camellia sinensis, 32 DEGs were discovered to be related to CGA biosynthesis, including sixteen phenylalanine ammonia lyase (PAL) genes, three 4-coumarate coenzyme A ligase (4CL) genes, nine cinnamate 4-Hydroxylase (C4H) genes, four Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HQT/HCT), and two hundred and twenty-one TFs including eighty-eight ERFs, forty-one bZIPs, forty-two MYBs and fifty WRKYs, which may also play an important role in the biosynthesis of CGA. Our results will lay the foundation for further exploration of the biosynthesis of CGA and revealing the related regulatory network in Camellia sinensis. © 2021 Friends Science Publishers

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Comparative transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis in Eucommia ulmoides Oliv.

Cited by 20 publications

References 50 publications

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Genetic Architecture Underlying the Metabolites of Chlorogenic Acid Biosynthesis in Populus tomentosa

Comparative Transcriptomic Analyses of Chlorogenic Acid Biosynthesis Pathways in Diploid and Triploid Camellia sinensis

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