Comparative Transcriptome-Based Mining of Senescence-Related MADS, NAC, and WRKY Transcription Factors in the Rapid-Senescence Line DLS-91 of Brassica rapa

Yi, So Young; Rameneni, Jana Jeevan; Lee, Myungjin; Song, Seul Gi; Choi, Yuri; Lu, Lu; Lee, Hyeokgeun; Lim, Yong Pyo

doi:10.3390/ijms22116017

Cited by 4 publications

(1 citation statement)

References 113 publications

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“…MADS-box is a conserved gene family found in plants as well as animals. Many studies have presented evidence for linkages between MADS-box members and senescence in different plant species; for example, Arabidopsis thaliana ( Fang and Fernandez, 2002 ), Solanum lycopersicum ( Xie et al., 2014 ), Medicago truncatula ( De Michele et al., 2009 ), and Brassica rapa ( Yi et al., 2021 ). The overexpression of MADS-box genes usually results in a delayed senescence process in different organs, which demonstrates the role of negative regulators of senescence.…”

Section: Introductionmentioning

confidence: 99%

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

2023

Front. Plant Sci.

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IntroductionLignification of fruit flesh is a common physiological disorder that occurs during post-harvest storage, resulting in the deterioration of fruit quality. Lignin deposition in loquat fruit flesh occurs due to chilling injury or senescence, at temperatures around 0°C or 20°C, respectively. Despite extensive research on the molecular mechanisms underlying chilling-induced lignification, the key genes responsible for the lignification process during senescence in loquat fruit remain unknown. MADS-box genes, an evolutionarily conserved transcription factor family, have been suggested to play a role in regulating senescence. However, it is still unclear whether MADS-box genes can regulate the lignin deposition that arises from fruit senescence.MethodsBoth senescence- and chilling-induced flesh lignification were simulated by applying temperature treatments on loquat fruits. The flesh lignin content during the storage was measured. Transcriptomic, quantitative reverse transcription PCR and correlation analysis were employed to identify key MADS-box genes that may be involved in flesh lignification. The Dual-luciferase assay was utilized to identify the potential interactions between MADS-box members and genes in phenylpropanoid pathway. Results and DiscussionThe lignin content of the flesh samples treated at 20°C or 0°C increased during storage, but at different rates. Results from transcriptome analysis, quantitative reverse transcription PCR, and correlation analysis led us to identify a senescence-specific MADS-box gene, EjAGL15, which correlated positively with the variation in lignin content of loquat fruit. Luciferase assay results confirmed that EjAGL15 activated multiple lignin biosynthesis-related genes. Our findings suggest that EjAGL15 functions as a positive regulator of senescence-induced flesh lignification in loquat fruit.

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Section: Introductionmentioning

confidence: 99%

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

2023

Front. Plant Sci.

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Functional validation of two B-class MADS-box genes HmPI and HmAP3 from Hemerocallis middendorffii

Shi,

Hou,

Dong

et al. 2024

In Vitro Cell.Dev.Biol.-Plant

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Comprehensive transcriptome profiling and transcription factor identification in early/late leaf senescence grafts in potato

He,

Li,

Hui

et al. 2024

Physiologia Plantarum

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Potato (Solanum tuberosum L.) is recognized globally as the most significant non‐cereal staple crop. Leaf senescence, which significantly impacts tuber yield, serves as a critical indicator of potato maturity. Despite its importance, the molecular mechanisms regulating this process remain largely unknown. In a previous study, we grafted the early‐maturing variety ‘Zhongshu 5’ (Z5) onto the late‐maturing variety ‘Zhongshu 18’ (Z18), and demonstrated that the rootstock's leaves displayed physiological characteristics suggestive of early senescence. Here, we analyzed the transcriptome data of the Z5 and Z18 grafts to conduct weighted gene co‐expression network and gene expression clustering analysis. Differentially expressed genes in cluster 9, as well as the floralwhite module, exhibited markedly elevated expression levels during the onset of leaf senescence. These genes were found to be enriched in several senescence related processes, such as chloroplast organization, electron transport chain, and chlorophyll metabolic process. Furthermore, we constructed transcription factor correlation networks and hub gene co‐expression networks. By monitoring the expression patterns of these genes throughout the whole growth period, we identified two candidate genes, StWRKY70 and StNAP, which may play pivotal roles in leaf senescence. This study contributes valuable genetic resources for further investigations into the regulatory mechanism governing potato leaf senescence, with implications for genetic improvements, particularly in terms of maturity and yield.

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Comparative Transcriptome-Based Mining of Senescence-Related MADS, NAC, and WRKY Transcription Factors in the Rapid-Senescence Line DLS-91 of Brassica rapa

Cited by 4 publications

References 113 publications

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

Functional validation of two B-class MADS-box genes HmPI and HmAP3 from Hemerocallis middendorffii

Comprehensive transcriptome profiling and transcription factor identification in early/late leaf senescence grafts in potato

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