Comparative transcriptome profiling of genes and pathways related to resistance against powdery mildew in two contrasting melon genotypes

Zhu, Qianglong; Gao, Peng; Wan, Yan; Cui, Haonan; Fan, Chao; Liu, Shi; Luan, Feishi

doi:10.1016/j.scienta.2017.09.033

Cited by 40 publications

(31 citation statements)

References 63 publications

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“…These results may suggest that the resistant genotype initiatively activated the early disease–response genes to Lxx infection by 36hpi. A resistant melon genotype similarly exhibited a faster response to Podosphaera xanthii infection than a susceptible genotype at the early stage of infection (Zhu et al, ).…”

Section: Resultsmentioning

confidence: 99%

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Comparative analysis reveals changes in transcriptomes of sugarcane upon infection by Leifsoniaxyli subsp. xyli

Wei

Pan

et al. 2019

Journal of Phytopathology

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Ratoon stunting disease (RSD) caused by bacterium Leifsoniaxyli subsp. xyli (Lxx) is a devastating disease of sugarcane over a large part of the world. Genetic improvement for RSD‐resistant varieties is considered the most effective method to control the disease. However, genetic improvement of sugarcane is hindered by the limited information about the molecular mechanisms underlying Lxx pathogenicity and defence responses in sugarcane. In this study, genome‐wide gene expression profiling was used to compare RSD‐resistant (CP72‐2086) and RSD‐susceptible (GT11) genotypes at different infection time points in order to identify the candidate regulators for RSD resistance. A total of 14,494 differentially expressed genes (DEGs) were identified, indicating that dramatic changes had occurred in gene expression upon Lxx infection, especially in the susceptible genotype. Enrichment analysis showed that a large number of genes related to plant hormone signal transduction, phenylalanine metabolism, phenylpropanoid biosynthesis and starch and sucrose metabolism was responsible for sugarcane response to Lxx infection. Plant hormone signalling pathway genes were significantly differentially expressed at the early infection stage between the two genotypes. The resistant genotype chose the jasmonic acid‐ and ethylene‐dependent host‐defence pathways to resist Lxx infection, whereas the susceptible genotype preferred the salicylic acid‐dependent host‐defence pathways. These findings help unravel the molecular mechanisms of sugarcane plant–Lxx interactions and may pave the way for sugarcane breeding for disease resistance.

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Section: Resultsmentioning

confidence: 99%

“…to Podosphaera xanthii infection than a susceptible genotype at the early stage of infection (Zhu et al, 2018).…”

Section: Identification Of Differentially Expressed Genesmentioning

confidence: 96%

Comparative analysis reveals changes in transcriptomes of sugarcane upon infection by Leifsoniaxyli subsp. xyli

Wei

Pan

et al. 2019

Journal of Phytopathology

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“…DM resistance has been identified using the mutants at DM genes in lettuce [39]. Interaction between genes and pathways related to resistance against powdery mildew (PM) in melon has been profiled through comparative transcriptome analysis [40]. In maize, most studies on DM resistance have been performed using QTL analysis.…”

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confidence: 99%

Identification and Validation of Candidate Genes Conferring Resistance to Downy Mildew in Maize (Zea mays L.)

Kim

Song

et al. 2020

Genes

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Downy mildew (DM) is a major disease of maize that causes significant yield loss in subtropical and tropical regions around the world. A variety of DM strains have been reported, and the resistance to them is polygenically controlled. In this study, we analyzed the quantitative trait loci (QTLs) involved in resistance to Peronosclerospora sorghi (sorghum DM), P. maydis (Java DM), and Sclerophthora macrospora (crazy top DM) using a recombinant inbred line (RIL) from a cross between B73 (susceptible) and Ki11 (resistant), and the candidate genes for P. sorghi, P. maydis, and S. macrospora resistance were discovered. The linkage map was constructed with 234 simple sequence repeat (SSR) and restriction fragment length polymorphism (RFLP) markers, which was identified seven QTLs (chromosomes 2, 3, 6, and 9) for three DM strains. The major QTL, located on chromosome 2, consists of 12.95% of phenotypic variation explained (PVE) and a logarithm of odds (LOD) score of 14.12. Sixty-two candidate genes for P. sorghi, P. maydis, and S. macrospora resistance were obtained between the flanked markers in the QTL regions. The relative expression level of candidate genes was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) using resistant (CML228, Ki3, and Ki11) and susceptible (B73 and CML270) genotypes. For the 62 candidate genes, 15 genes were upregulated in resistant genotypes. Among these, three (GRMZM2G028643, GRMZM2G128315, and GRMZM2G330907) and AC210003.2_FG004 were annotated as leucine-rich repeat (LRR) and peroxidase (POX) genes, respectively. These candidate genes in the QTL regions provide valuable information for further studies related to P. sorghi, P. maydis, and S. macrospora resistance.Genes 2020, 11, 191 2 of 20 is spread by oospores that survive in the soil [7] and can be spread through infected seeds or from plant-to-plant by airborne conidia. Because of the systemic nature of DM, susceptible lines usually die when infected in the seedling emergence stage, and when plants are infected during later growth stages, they cannot develop the maize ear despite having survived. At least six pathogens that cause DM infection of maize in Asia have been reported, including sorghum DM (P. sorghi (Weston & Uppal)), Philippine DM (P. philippinensis (Weston) Shaw), Java DM (P. maydis (Raciborski)), sugarcane DM (P. sacchari (Miyabe) Shirai and Hara), brown stripe DM (Sclerophthora rayssiae var. zeae), crazy top DM (S. macrospora), and Rajasthan DM (P. heteropogoni) [2,6,[8][9][10][11][12][13][14][15]. DM is widespread in tropical regions, although its origin is conjectural, and because of the diversity of the DM pathogens and their systemic nature, the development of resistant varieties is needed. Moreover, a renewed emphasis on cost-effectiveness and environmental safety that has brought about the application of DM management by the development of resistant varieties. According to studies on the interaction of maize and the pathogens, resistance to DM is polygenically controlled [7,13,[16][17][18]...

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“…Similar results were reported in litchi [46], in which TFs were upregulated in response to ethephon during fruit abscission. These TFs are activated and subsequently regulate both the production of downstream stress defenses and the formation of protection [46][47]. Within two contrasting melon genotypes with respect to sensitivity to powdery mildew, TFs responded more strongly to pathogen attack in the resistant genotypes after Podosphaera xanthii inoculation [47].…”

Section: More Tfs Responded To Ethephon Stimulation In Sc After Ethepmentioning

confidence: 99%

“…These TFs are activated and subsequently regulate both the production of downstream stress defenses and the formation of protection [46][47]. Within two contrasting melon genotypes with respect to sensitivity to powdery mildew, TFs responded more strongly to pathogen attack in the resistant genotypes after Podosphaera xanthii inoculation [47]. In the present work, more TFs responded to ethephon stimulation in SC (in which relatively greater expression changes occurred) than in CP, which suggested that SC responded more strongly to defend against the ethephon stimulation stress.…”

Section: More Tfs Responded To Ethephon Stimulation In Sc After Ethepmentioning

confidence: 99%

Comparative Analyses of the Transcriptome and Proteome of Comte de Paris and Smooth Cayenne to Improve the Understanding of Ethephon-Induced Floral Transition in Pineapple

Liu¹,

Liu²,

Shao³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ethylene is usually used to induce floral transition in pineapple. However, its successful induction in plants categorized as Cayenne is difficult or completely ineffective, and information concerned is limited. The present study was undertaken to investigate the molecular mechanisms underlying this obstacle. Methods: Transcriptome and proteome comparative analyses were performed to explore the important regulation and pathway variations after ethephon induction in the induction-easy ‘Comte de Paris’ (CP) and induction-hard ‘Smooth Cayenne’ (SC) cultivars via RNA-seq (RNA-sequencing) and iTRAQ (isobaric tags for relative and absolute quantification). Results: CP and SC exhibited basic differences at the transcriptomic and proteomic levels before ethephon treatment, including the expression of genes and proteins related to ethylene signal transduction. After ethephon induction, the expression of genes and proteins involved in plant ethylene signal transduction and carbohydrate metabolism responded more strongly in CP than in SC. The expression of the floral meristem identity (FMI) genes AG, TFL and FT exhibited greater changes in CP, and more transcription factors responded in SC. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many differentially expressed genes (DEGs) in CP were annotated to terms and pathways involved in photoperiodism and shared components involved in carbohydrate metabolism and plant hormone signal transduction. Conclusion: These findings contribute to the understanding of the molecular mechanism underlying the variation between CP and SC in response to ethephon-mediated floral induction.

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Comparative transcriptome profiling of genes and pathways related to resistance against powdery mildew in two contrasting melon genotypes

Cited by 40 publications

References 63 publications

Comparative analysis reveals changes in transcriptomes of sugarcane upon infection by Leifsoniaxyli subsp. xyli

Comparative analysis reveals changes in transcriptomes of sugarcane upon infection by Leifsoniaxyli subsp. xyli

Identification and Validation of Candidate Genes Conferring Resistance to Downy Mildew in Maize (Zea mays L.)

Comparative Analyses of the Transcriptome and Proteome of Comte de Paris and Smooth Cayenne to Improve the Understanding of Ethephon-Induced Floral Transition in Pineapple

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