Comparative transcriptomic analysis identified differentially expressed genes and pathways involved in the interaction between Tremella fuciformis and Annulohypoxylon stygium

Li, Dongmei; Pujiana, Dwi; Wang, Yuanyuan; Zhang, Zhao-Song; Zheng, Liesheng; Chen, Liguo; Ma, Aimin

doi:10.1007/s10482-019-01294-4

Cited by 6 publications

(6 citation statements)

References 34 publications

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“…Another species showing the same pattern of presence and role of M. polyspora is Annulohypoxylon stygium (Xylariales, Ascomycota), a white-rot fungus commonly found on dead wood (Liu et al, 2019). This fungus, like M. polyspora, was overrepresented in T-NR and T-R soils and was an indicator species of the site group combination NR(T-NT).…”

Section: Differential Responses Of Soil Fungi To Management Systemsmentioning

confidence: 99%

“…This fungus, like M. polyspora, was overrepresented in T-NR and T-R soils and was an indicator species of the site group combination NR(T-NT). A. stygium shows exceptionally high performance in lignin and carbohydrate degradation (Liu et al, 2019). Interestingly, this fungus was identified to be the main symbiotic species of Tremella fuciformis, medicinal and culinary fungi of China which is a fungus that does not form an edible basidiome without the presence of a specific host fungus both in nature and for industrial production (Liu et al, 2019).…”

Section: Differential Responses Of Soil Fungi To Management Systemsmentioning

confidence: 99%

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How Tillage and Crop Rotation Change the Distribution Pattern of Fungi

Orrù¹,

Canfora²,

Trinchera³

et al. 2021

Front. Microbiol.

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Massive sequencing of fungal communities showed that climatic factors, followed by edaphic and spatial variables, are feasible predictors of fungal richness and community composition. This study, based on a long-term field experiment with tillage and no-tillage management since 1995 and with a crop rotation introduced in 2009, confirmed that tillage practices shape soil properties and impact soil fungal communities. Results highlighted higher biodiversity of saprotrophic fungi in soil sites with low disturbance and an inverse correlation between the biodiversity of ectomycorrhizal and saprotrophic fungi. We speculated how their mutual exclusion could be due to a substrate-mediated niche partitioning or by space segregation. Moreover, where the soil was ploughed, the species were evenly distributed. There was higher spatial variability in the absence of ploughing, with fungal taxa distributed according to a small-scale pattern, corresponding to micro-niches that probably remained undisturbed and heterogeneously distributed. Many differentially represented OTUs in all the conditions investigated were unidentified species or OTUs matching at high taxa level (i.e., phylum, class, order). Among the fungi with key roles in all the investigated conditions, there were several yeast species known to have pronounced endemism in soil and are also largely unidentified. In addition to yeasts, other fungal species emerged as either indicator of a kind of management or as strongly associated with a specific condition. Plant residues played a substantial role in defining the assortment of species.

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Section: Differential Responses Of Soil Fungi To Management Systemsmentioning

confidence: 99%

Section: Differential Responses Of Soil Fungi To Management Systemsmentioning

confidence: 99%

How Tillage and Crop Rotation Change the Distribution Pattern of Fungi

Orrù¹,

Canfora²,

Trinchera³

et al. 2021

Front. Microbiol.

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show abstract

“…Mitogen-activated protein kinases (MAPKs) are key mediators of signaling in fungi, participating in the response to diverse stresses and developmental processes ( Liu D. et al, 2019 ). We screened MAPK signaling pathway - yeast genes and observed upregulated expression of a catalase gene ( evm.TU.contig_3_pilon.1115 ) and downregulated expression of Pheromone B alpha 3 receptor ( evm.TU.contig_25_pilon.509 ) and GTP-binding protein rhoA ( evm.TU.contig_32_pilon.1268 ) genes, and so on.…”

Section: Discussionmentioning

confidence: 99%

Genome and Comparative Transcriptome Dissection Provide Insights Into Molecular Mechanisms of Sclerotium Formation in Culinary-Medicinal Mushroom Pleurotus tuber-regium

Sun

Zhang

et al. 2022

Front. Microbiol.

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Pleurotus tuber-regium is an edible and medicinal sclerotium-producing mushroom. The sclerotia of this mushroom also serve as food and folk medicine. Based on the description of its monokaryon genome, sequenced with Illumina and PacBio sequencing technologies, comparative transcriptomic analysis using RNA sequencing (RNA-seq) was employed to study its mechanism of sclerotium formation. The de novo assembled genome is 35.82 Mb in size with a N50 scaffold size of 4.29 Mb and encodes 12,173 putative proteins. Expression analysis demonstrated that 1,146 and 1,249 genes were upregulated and downregulated with the formation of sclerotia, respectively. The differentially expressed genes were associated with substrate decomposition, the oxidation-reduction process, cell wall synthesis, and other biological processes in P. tuber-regium. These genomic and transcriptomic resources provide useful information for the mechanism underlying sclerotium formation in P. tuber-regium.

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“…It exhibits a high ability to degrade lignin and carbohydrates (Hsieh et al 2005 ; Wingfield et al 2018 ). As an associated fungus, A. stygium provides nutrition for the growth and development environments of Tremella fuciformis and is commonly found in natural and artificial cultivation environments (Deng et al 2016 ; Liu et al 2019 ). More than 500 kt of fresh T. fuciformis were produced in 2020 (Sun 2023 ), generating more than 800 kt of spent mushroom compost, most of which contains only A. stygium mycelium, just less than 5% being a mixture of the two fungi.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive investigations of 2-phenylethanol production by the filamentous fungus Annulohypoxylon stygium

Tong,

Yang,

Zhang

et al. 2024

Appl Microbiol Biotechnol

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2-Phenylethanol (2-PE) is an aromatic compound with a rose-like fragrance that is widely used in food and other industries. Yeasts have been implicated in the biosynthesis of 2-PE; however, few studies have reported the involvement of filamentous fungi. In this study, 2-PE was detected in Annulohypoxylon stygium mycelia grown in both potato dextrose broth (PDB) and sawdust medium. Among the 27 A. stygium strains investigated in this study, the strain “Jinjiling” (strain S20) showed the highest production of 2-PE. Under optimal culture conditions, the concentration of 2-PE was 2.33 g/L. Each of the key genes in Saccharomyces cerevisiae shikimate and Ehrlich pathways was found to have homologous genes in A. stygium. Upon the addition of L-phenylalanine to the medium, there was an upregulation of all key genes in the Ehrlich pathway of A. stygium, which was consistent with that of S. cerevisiae. A. stygium as an associated fungus provides nutrition for the growth of Tremella fuciformis and most spent composts of T. fuciformis contain pure A. stygium mycelium. Our study on the high-efficiency biosynthesis of 2-PE in A. stygium offers a sustainable solution by utilizing the spent compost of T. fuciformis and provides an alternative option for the production of natural 2-PE. Key points • Annulohypoxylon stygium can produce high concentration of 2-phenylethanol. • The pathways of 2-PE biosynthesis in Annulohypoxylon stygium were analyzed. • Spent compost of Tremella fuciformis is a potential source for 2-phenylethanol.

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Comparative transcriptomic analysis identified differentially expressed genes and pathways involved in the interaction between Tremella fuciformis and Annulohypoxylon stygium

Cited by 6 publications

References 34 publications

How Tillage and Crop Rotation Change the Distribution Pattern of Fungi

How Tillage and Crop Rotation Change the Distribution Pattern of Fungi

Genome and Comparative Transcriptome Dissection Provide Insights Into Molecular Mechanisms of Sclerotium Formation in Culinary-Medicinal Mushroom Pleurotus tuber-regium

Comprehensive investigations of 2-phenylethanol production by the filamentous fungus Annulohypoxylon stygium

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