Comparative transcriptomic analysis identifies reprogramming and differentiation genes differentially expressed in UiPSCs and ESCs

Shi, Liang; Cui, Yazhou; Zhou, Xiaoyan; Luan, Jing; Wang, Linli; Han, Jinxiang

doi:10.5582/bst.2017.01082

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…In addition, several studies have found that UiPSCs preferentially differentiate into neurons and that even epithelial-like cells from human urine can directly differentiate into neural progenitor cells (20,21). Although studies have suggested that irreversible nerve damage due to a high concentration of phenylalanine in the blood may be related to brain-derived neurotrophic factor (22,23), no studies have thoroughly examined the mechanism whereby hyperphenylalaninemia results in mental retardation.…”

Section: Discussionmentioning

confidence: 99%

Generation of urine-derived induced pluripotent stem cells from a patient with phenylketonuria

Cui

Shi

et al. 2018

IRDR

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The aim of the study was to establish an induced pluripotent stem cell line from urine-derived cells (UiPSCs) from a patient with phenylketonuria (PKU) in order to provide a useful research tool with which to examine the pathology of this rare genetic metabolic disease. Urine-derived epithelial cells (UCs) from a 15-year-old male patient with PKU were isolated and reprogrammed with integration-free episomal vectors carrying an OCT4, SOX2, KLF4, and miR-302-367 cluster. PKU-UiPSCs were verified as correct using alkaline phosphatase staining. Pluripotency markers were detected with real-time PCR and flow cytometry. Promoter methylation in two pluripotent genes, and, was analyzed using bisulphite sequencing. An embryoid body (EB) formation assay was also performed. An induced pluripotent stem cell line (iPSC) was generated from epithelial cells in urine from a patient with PKU. This cell line had increased expression of stem cell biomarkers, it efficiently formed EBs, it stained positive for alkaline phosphatase (ALP), and it had a marked decrease in promoter methylation in the and genes. The PKU-UiPSCs created here had typical characteristics and are suitable for further differentiation.

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Section: Discussionmentioning

confidence: 99%

Generation of urine-derived induced pluripotent stem cells from a patient with phenylketonuria

Cui

Shi

et al. 2018

IRDR

Self Cite

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Application prospects of urine-derived stem cells in neurological and musculoskeletal diseases

Yang,

Zheng,

Bai

et al. 2024

World J Orthop

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Urine-derived stem cells (USCs) are derived from urine and harbor the potential of proliferation and multidirectional differentiation. Moreover, USCs could be reprogrammed into pluripotent stem cells [namely urine-derived induced pluripotent stem cells (UiPSCs)] through transcription factors, such as octamer binding transcription factor 4, sex determining region Y-box 2, kruppel-like factor 4, myelocytomatosis oncogene, and Nanog homeobox and protein lin-28, in which the first four are known as Yamanaka factors. Mounting evidence supports that USCs and UiPSCs possess high potential of neurogenic, myogenic, and osteogenic differentiation, indicating that they may play a crucial role in the treatment of neurological and musculoskeletal diseases. Therefore, we summarized the origin and physiological characteristics of USCs and UiPSCs and their therapeutic application in neurological and musculoskeletal disorders in this review, which not only contributes to deepen our understanding of hallmarks of USCs and UiPSCs but also provides the theoretical basis for the treatment of neurological and musculoskeletal disorders with USCs and UiPSCs.

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Comparative transcriptomic analysis identifies reprogramming and differentiation genes differentially expressed in UiPSCs and ESCs

Cited by 2 publications

References 25 publications

Generation of urine-derived induced pluripotent stem cells from a patient with phenylketonuria

Generation of urine-derived induced pluripotent stem cells from a patient with phenylketonuria

Application prospects of urine-derived stem cells in neurological and musculoskeletal diseases

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