2018
DOI: 10.1111/1744-7917.12561
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Comparative transcriptomic analysis of larval and adult Malpighian tubules from the cotton bollworm Helicoverpa armigera

Abstract: Malpighian tubules (MTs) are usually considered the key excretory and osmoregulatory organs of insects. However, increasing evidence has suggested that MTs perform many more functions than just osmoregulation. Until now, the molecular and physiological functions of MTs in the cotton bollworm (Helicoverpa armigera), a very important agricultural pest, are largely unknown. In this study, the transcriptomes of H. armigera MTs from larvae, male adults and female adults were sequenced using RNA-Seq technology, and … Show more

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Cited by 13 publications
(10 citation statements)
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“…Correlations between the transcriptomes in many tissues or organs of animals (e.g., antennae, salivary glands, midgut, midgut ‘appendices’, hepatopancreas, venom gland, gill) and its respective physiological functions have been reported [ 40 45 ]. Similar studies have been carried out for the MTs of a few insect species, but these studies have been limited in viewing the MTs as a whole organ [ 3 , 5 , 10 , 12 , 22 , 25 , 46 50 ]. To our knowledge, studies of the transcriptome of vector leafhoppers have mainly focused on the salivary glands and the alimentary tract [ 51 55 ].…”
Section: Discussionmentioning
confidence: 99%
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“…Correlations between the transcriptomes in many tissues or organs of animals (e.g., antennae, salivary glands, midgut, midgut ‘appendices’, hepatopancreas, venom gland, gill) and its respective physiological functions have been reported [ 40 45 ]. Similar studies have been carried out for the MTs of a few insect species, but these studies have been limited in viewing the MTs as a whole organ [ 3 , 5 , 10 , 12 , 22 , 25 , 46 50 ]. To our knowledge, studies of the transcriptome of vector leafhoppers have mainly focused on the salivary glands and the alimentary tract [ 51 55 ].…”
Section: Discussionmentioning
confidence: 99%
“…MTs express plenty of organic solute transporters, indicating the tubules have the capability to actively excrete the broadest range of organic solutes (e.g., sugar, amino acid and multivitamin) [ 4 ]. MTs express multiple detoxification-related genes, e.g., cytochrome P450 monooxygenases (P450s), glutathione S -transferases (GSTs), alcohol dehydrogenases (ADHs), uridine diphosphate-glycosyltransferases (UGTs) and ATP-binding cassette (ABC) transporters, suggesting that the tubules have physiological functions in the detoxification of endogenous secondary metabolites and xenobiotics (e.g., plant allelochemicals: ouabain, salicylate and vinblastine) or toxins (e.g., insecticides) [ 5 8 ]. MTs also express a variety of immunity-related genes involved in immunological responses, which sense pathogenic challenge (e.g., bacteria, fungi, viruses and protozoa) and mount effective killing responses by triggering the generation of effector activities (phagocytosis) and effector molecules (e.g., attacin, defensin and cecropin) [ 9 ].…”
Section: Introductionmentioning
confidence: 99%
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“…Until relatively recently, our understanding of diuresis in insects relied heavily on in vitro work, which has indeed enabled models to be developed on the neurohormonal control of diuresis. Access to the insect genomes that have been sequenced over the last 20 years or so, and advances in molecular techniques, have, however, revolutionized insect (neuro)endocrinology, and functional genomics and genetics, including gene micro-arrays, transcriptomes, mutations, double stranded RNA (dsRNA) and sophisticated peptidomics ('omics in general) are enabling complex regulatory processes to be exquisitely dissected and defined (2,4,(9)(10)(11)(12)(13). So too, the basic model of post-prandial diuresis in R. prolixus, with its diuretic and antidiuretic hormones, coupled to the second messengers cAMP, cGMP and IP 3 , and in turn acting via predicted ion channels, transporters and aquaporins is here validated and confirmed by transcriptomics; but transcriptomics has also revealed how much more still needs to be understood.…”
Section: Final Thoughtsmentioning
confidence: 99%
“…The post-genomic era has brought forth great advances in our understanding of the diuretic processes in insects, enabling the identification and quantification of thousands of target genes in a single experiment, as well as enabling comparative genomic studies. Microarray and transcriptome analyses of Malpighian tubules have also reinforced the major roles that Malpighian tubules play in detoxification and immunity, and in tolerance to stress and desiccation (2,4,(9)(10)(11)(12)(13). The R. prolixus genome has been sequenced and reported (14), bringing the post-genomic era to bear on this historic model insect.…”
Section: Introductionmentioning
confidence: 96%