Compared selectivities of the phosphatidylinositol-synthase from maize coleoptiles either in microsomal membranes or after solubilization

Justin, Anne-Marie; Hmyene, Abdelaziz; Kader, Jean‐Claude; Mazliak, P.

doi:10.1016/0005-2760(94)00234-p

Cited by 21 publications

(17 citation statements)

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“…Indeed, it has been indicated that PI synthase from the organs of different plants shows a high selectivity for the synthesis of saturated-unsaturated PI species (27). Moreover, similar results were observed in maize coleoptile microsomes (28) and in E. coli expressing an exogenous PI synthase from Arabidopsis (29). The importance and function of this enrichment in saturated FA of PI has not been yet determined.…”

Section: Resultssupporting

confidence: 67%

Phospholipid molecular profiles in the seed kernel from different sunflower (Helianthus annuus) mutants

Salas

Martı́nez-Force

Garcés

2006

Lipids

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Phospholipids are essential components of plant cell membranes whose acyl composition appears to be influenced by oil composition in the sunflower. In the current study, we have determined the diacylglycerol profile of the main phospholipids using phospholipase C degradation and separation of the diacylglycerols by HPLC and GLC. The main polar lipid molecular species were defined in different classes of sunflower kernel: PC, PE, and PI. The proportions of each were determined at different stages of development in order to define the point at which the mutations carried by each sunflower line affected the phospholipid composition of the seeds. The results indicated that modifications to intraplastidial de novo FA synthesis affected the seed phospholipid profile during the whole period of the seed formation, including accumulation and maturation, whereas the influence of mutations in the endoplasmic reticulum desaturases were more readily detected at later stages of development. These results are discussed in terms of the pathways involved in glycerolipid synthesis and phospholipid conversion in sunflower seeds.

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Section: Resultssupporting

confidence: 67%

Phospholipid molecular profiles in the seed kernel from different sunflower (Helianthus annuus) mutants

Salas

Martı́nez-Force

Garcés

2006

Lipids

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“…After separation by TLC according to Lepage [15], the PtdIns spot was scrapped and PtdIns eluted at 8 °C overnight from the silica in 5 mL methanol containing one drop of glacial acetic acid. After re‐extraction of the sample according to Bligh & Dyer [16], PtdIns molecular species were analysed by radio‐HPLC as described previously [17].…”

Section: Methodsmentioning

confidence: 99%

Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis

Justin

Kader

Collin

2002

European Journal of Biochemistry

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In order to study some of its enzymatic properties, phosphatidylinositol synthase 1 (AtPIS1) from the plant Arabidopsis thaliana was expressed in Escherichia coli, a host naturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Our data clearly show that the CDP-diacylglycerol-independent exchange reaction can occur using endogenous PtdIns molecular species or PtdIns molecular species from soybean added exogenously. Exchange has been observed in the absence of cytidine monophosphate (CMP), but is greatly enhanced in the presence of 4 lM CMP. Our data also show that AtPIS1 catalyses the removal of the polar head in the presence of much higher concentrations of CMP, in a manner that suggests a reverse of synthesis. All of the PtdIns metabolizing activities require free manganese ions. EDTA, in the presence of low Mn 2+ concentrations, also has an enhancing effect.

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“…1B). For instance, PI-synthases from different model systems including Arabidopsis have been demonstrated to localise to the ER and, possibly, Golgi (Fischl & Carman 1983;Justin et al 1995;Leber et al 1995;Collin et al 1999;L€ ofke et al 2008); Arabidopsis PI4-kinase b1 (encoded by gene locus At5g64070; Xue et al 1999) has been shown to localise to the trans-Golgi-network (Preuss et al 2006;Ischebeck et al 2010b), and Arabidopsis PI4P 5-kinases have been shown to localise in the plasma membrane and, possibly, secretory vesicles (Ischebeck et al , 2011Kusano et al 2008;Sousa et al 2008;Stenzel et al 2008Stenzel et al , 2012Camacho et al 2009). Thus, enzymes mediating the production of PIs display only partial spatial overlap, if any.…”

Section: Alternative Functions Of Pis In Different Organelles and Thementioning

confidence: 99%

Arranged marriage in lipid signalling? The limited choices ofPtdIns(4,5)P₂in finding the right partner

Heilmann

2013

Plant Biol J

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Inositol-containing phospholipids (phosphoinositides, PIs) control numerous cellular processes in eukaryotic cells. For plants, a key involvement of PIs has been demonstrated in the regulation of membrane trafficking, cytoskeletal dynamics and in processes mediating the adaptation to changing environmental conditions. Phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)) mediates its cellular functions via binding to various alternative target proteins. Such downstream targets of PtdIns(4,5)P(2) are characterised by the possession of specific lipid-binding domains, and binding of the PtdIns(4,5)P(2) ligand exerts effects on their activity or localisation. The large number of potential alternative binding partners - and associated cellular processes - raises the question how alternative or even contrapuntal effects of PtdIns(4,5)P(2) are orchestrated to enable cellular function. This article aims to provide an overview of recent insights and new views on how distinct functional pools of PtdIns(4,5)P(2) are generated and maintained. The emerging picture suggests that PtdIns(4,5)P(2) species containing different fatty acids influence the lateral mobility of the lipids in the membrane, possibly enabling specific interactions of PtdIns(4,5)P(2) pools with certain downstream targets. PtdIns(4,5)P(2) pools with certain functions might also be defined by protein-protein interactions of PI4P 5-kinases, which pass PtdIns(4,5)P(2) only to certain downstream partners. Individually or in combination, PtdIns(4,5)P(2) species and specific protein-protein interactions of PI4P 5-kinases might contribute to the channelling of PtdIns(4,5)P(2) signals towards specific functional effects. The dynamic nature of PI-dependent signalling complexes with specific functions is an added challenge for future studies of plant PI signalling.

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Compared selectivities of the phosphatidylinositol-synthase from maize coleoptiles either in microsomal membranes or after solubilization

Cited by 21 publications

References 17 publications

Phospholipid molecular profiles in the seed kernel from different sunflower (Helianthus annuus) mutants

Phospholipid molecular profiles in the seed kernel from different sunflower (Helianthus annuus) mutants

Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis

Arranged marriage in lipid signalling? The limited choices ofPtdIns(4,5)P₂in finding the right partner

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Compared selectivities of the phosphatidylinositol-synthase from maize coleoptiles either in microsomal membranes or after solubilization

Cited by 21 publications

References 17 publications

Phospholipid molecular profiles in the seed kernel from different sunflower (Helianthus annuus) mutants

Phospholipid molecular profiles in the seed kernel from different sunflower (Helianthus annuus) mutants

Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis

Arranged marriage in lipid signalling? The limited choices ofPtdIns(4,5)P2in finding the right partner

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Arranged marriage in lipid signalling? The limited choices ofPtdIns(4,5)P₂in finding the right partner