Comparing normal primary endocervical adenoepithelial cells to uninfected and influenza B virus infected human cervical adenocarcinoma HeLa cells

Abstract

Human adenocarcinoma HeLa cells surviving infection with low (10(-9) units), medium (10(-6) units), and high (10(-2) units) influenza B titers were compared to their uninfected precursors and to normal endocervical adenoepithelial and metaplastic cells using Papanikolaou-staining method and immunocytochemistry. Normal primary endocervical and infected HeLa cells surviving infection shared similar morphologic, phenotypic, and divisional patterns that differed drastically from those of uninfected HeLa cells. The number of infected hosts surviving 6-7 days of viral exposure did not change during 3-week follow-up period, and their cyclin E levels suggested that they had been arrested to the G1 phase of the cell cycle by viral stress. Our findings suggest that in addition to apoptosis, nononcogenic viral stress activated the expression of endocervical metaplastic-like motifs in surviving hosts. A mechanism of cell response to nononcogenic viral stress was proposed to explain these findings. We conclude that nononcogenic respiratory viruses specifically target and eliminate abnormal cells ectopically overexpressing appropriate receptors and may complement current treatments of cervical cancer.

Immunophenotypic Evaluation of DNA Mismatch Repair Markers in 2 Cases of Synchronous Histomorphologically Distinct Gastric Adenocarcinomas With Gastrointestinal Stromal Tumors of the Proximal Small Bowel

“…8 Specific antibody reaction signals were evaluated relative to negative and positive controls. Negative controls of all tissue sections examined were treated with 1% bovine serum albumin instead of primary antibody.…”

Role of Hyperglycemia in Isogeneic Islet Transplantation: An Experimental Animal Study

“…Removed kidneys were fi xed in formol saline. Paraffi n sections were stained with hematoxylin-eosin and aldehyde fuchsin ( Nomikos et al, 1986 ;Sioutopoulou et al, 2006 ). Immunoperoxidase was used for insulin and glycagon staining Vakalis et al, 1999 ).…”

The Fat Content of Small Primary Breast Cancer Interferes with Radiofrequency-Induced Thermal Ablation

“…Tumor diameter was measured preoperatively by ultrasound scanning and postoperatively in the pathology laboratory. Core biopsy specimens were stained by H/E for histological diagnosis and evaluated immunocytochemically for HER-2/neu, estrogen and progesterone receptor content [6,7] . All participants signed an informed consent form and the ethics committee of Thessalia University Hospital approved this protocol.…”