Comparing the cytotoxic effect of light-emitting and organic light-emitting diodes based light therapy on human adipose-derived stem cells

Fibronectin-Enriched Interface Using a Spheroid-Converged Cell Sheet for Effective Wound Healing

“…Therefore, excessive ROS generation normally induces apoptotic activity of the cells. 40,41 Therefore, the light dose must be adjusted so that the amount of ROS generated falls below the toxic range. 40−42 In this study, we aimed to manufacture a microstructure (MS) patch consisting of cell sheets and spheroid structures to culture human mesenchymal stem cell complexes (hMSCcx), with high cell loading efficacy, angiogenic activity, and ROS tolerance.…”

“…Because light energy can be transferred to designated tissue or cells intact owing to its remote and penetrative properties, light has attracted significant attention as a strong tool for future biomedical technology. − Light has also been adopted as a cell preconditioning tool because it induces the angiogenic secretive properties of cells by inducing ROS signaling. ,, ROS signaling in light therapy is achieved by ROS generation, following stimulation of cytochrome c oxidase in the mitochondria, which results in HIF-1α upregulation. , Because light can induce phototoxicity through excessive ROS generation, where ROS production exceeds elimination resulting in loss of ROS homeostasis, it is important to strike balance between ROS signaling and phototoxicity in light therapy. Therefore, excessive ROS generation normally induces apoptotic activity of the cells. , Therefore, the light dose must be adjusted so that the amount of ROS generated falls below the toxic range. − …”