Comparing the effect of tempe flour and tofu flour consumption on estrogen serum in ovariectomized rats

Kridawati, Atik; Rahardjo, Tri Budi W.; Hardinsyah, .; Damanik, Rizal; Hogervorst, Eef

doi:10.1016/j.heliyon.2019.e01787

Cited by 9 publications

(9 citation statements)

References 18 publications

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“…The analysis results showed that the control group and the OH group had lower estradiol than the IPS and IPTS groups ( Table 2 ). These results are similar to previous studies that there was decrease in serum estrogen in OVx rat aged 12 months compared to non-OVx rat [ 40 ]. Another study showed that serum estradiol decreased in the postmenopause phase [ 2 ].…”

Section: Resultssupporting

confidence: 92%

Isoflavone and Antioxidant of Instant Cream Soup Made from Pumpkin and Tempeh and Their Active Compound in Ovariohysterectomy Rat-Induced Alzheimer’s Disease

Setiawan

Aulia

Sulaeman

et al. 2022

International Journal of Food Science

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Menopause period associated with brain function disorders can caused by decreasing estradiol levels and increasing oxidative stress in the body. Antioxidant agents are required to balance oxidative stress in the body. Instant cream soup made from pumpkin and tempeh is a supplementary food containing isoflavone and antioxidant agents. This study is aimed at analyzing the content of antioxidant level and isoflavones (genistein and daidzein) in instant cream soup and their effect on ovariohysterectomy (OVx) rats. Instant pumpkin cream soup with and without tempeh were subjected to isoflavone content and antioxidant analysis. Serum estradiol was analyzed using enzyme-linked immunosorbent assay. Liver concentration of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) were measured by spectrophotometric methods. The study showed that the isoflavone content (genistein 370.86 g/100 g, daidzein 185.61 g/100 g) was only present in the pumpkin instant cream soup with tempeh (IPTS). IPTS has higher antioxidant levels (134.25 mg AEAC/100 g) than instant pumpkin cream soup without tempeh (IPS). In vivo study, experimental rats showed that OVx increased malondialdehyde (MDA) levels up to 5.85-6.07 nmol mL-1 as compared to control (4.47 nmol mL-1). Moreover, instant pumpkin cream soup with tempeh treatments significantly increased serum estradiol levels (2.37–3.63 μg) and superoxide dismutase (SOD) levels of 497.49-558.89 U mL-1. This study concluded that instant pumpkin cream soup and tempeh contained isoflavone and antioxidant, and it increased estradiol serum and SOD level.

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Section: Resultssupporting

confidence: 92%

Isoflavone and Antioxidant of Instant Cream Soup Made from Pumpkin and Tempeh and Their Active Compound in Ovariohysterectomy Rat-Induced Alzheimer’s Disease

Setiawan

Aulia

Sulaeman

et al. 2022

International Journal of Food Science

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“…It is known that after the cessation of estrogen production by the ovaries, E2 biosynthesis takes place in peripheral tissues, especially adipose tissue, through aromatase conversion of androgens in postmenopausal women (12). Several studies have shown that PEs elevate the levels of E2 in female mice, and BZBS can also increase serum E2 levels (13,14). This is likely because PEs can competitively bind to ERα and ERβ, and the amount of ER in plasma is limited.…”

Section: Discussionmentioning

confidence: 99%

“…This is likely because PEs can competitively bind to ERα and ERβ, and the amount of ER in plasma is limited. Endogenous estrogen cannot bind to the receptor and will be unbound in the plasma, leading to an increase in E2 plasma levels (13,15). As a traditional Chinese medicine, BZBS is composed of a complex molecular mixture and has multi-target effects.…”

Section: Discussionmentioning

confidence: 99%

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Chinese medicine Bazi Bushen capsule improves lipid metabolism in ovariectomized female ApoE−/− mice

Huang

Hu²,

Chang³

et al. 2020

Ann Palliat Med

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Background: Dyslipidemia is one of the mechanisms of atherosclerosis (AS). Depletion of estrogen plays a key role in the pathogenesis of postmenopausal AS in women, and the blood lipid levels of women are closely related to endogenous estrogen levels. Phytoestrogens (PEs) exert estrogenic effects, including protection against AS, without the adverse effects of estrogen administration. Bazi Bushen capsule (BZBS) is a traditional Chinese medicine herbal compound prescription that has been shown to contain 11 unique PEs.In the present study, we assessed the effects of BZBS against lipid metabolism disorders.Methods: All ApoE -/mice underwent ovary ligation and bilateral ovariectomy (Ovx) to induce surgical menopause (Ovx/ApoE -/mice), whereas the C57BL/6J mice underwent sham surgery (needle threading).Ovx/ApoE -/mice were given a high-fat diet without estrogen and C57BL/6J mice were given a normal diet for 12 weeks. Ovx/ApoE -/mice treated with G1, a highly selective G-protein-coupled estrogen receptor1 (GPER1) agonist with proven activity against AS, were used as positive controls. Estrogen levels were measured and uterine atrophy index was calculated to determine the success of the model. Serum levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were measured in each group. The orthogonal projections to latent structures discriminant analysis (OPLS-DA) model was used to separate the groups, MetaboAnalyst was then used to analyze the metabolic pathway, and the most representative metabolites were finally identified.Results: Removal of bilateral ovaries resulted in successful surgical menopause models, where BZBS increased estrogen levels but did not increase the risk of uterine proliferation. BZBS attenuated dyslipidemia, including decreased TG, TC, and LDL-C levels, but increased HDL-C levels. The OPLS-DA model successfully distinguished the groups with good predictive ability and revealed their tendency to separate from each other. MetaboAnalyst suggested that both the G1 group and high-dose BZBS (HD-BZ) could improve the effect of lipid metabolism: the glycerophospholipid metabolism pathway was mainly improved by the G1 group, while the inositol phosphate metabolism pathway was mainly improved by the HD-BZ group. For the four compounds with the highest content, the concentrations of docosahexaenoic acid (DHA), 3-hydroxybutyric acid, and 5(Z), 8(Z), 11(Z)-eicosatrienoic acid were dramatically lower in the model group compared to the control group. Lysophosphatidylethanolamine (18:0) was higher in the model group than in the control group. BZBS corrected these effects.Conclusions: BZBS treatment reduced serum lipid levels and improved fatty acid metabolism in high-fat diet-fed, surgically induced menopausal ApoE -/mice.

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“…In Indonesia, tempe has been one of the most consumed protein sources for hundreds of years and known to be more preferable than meat and poultry [ 5 ]. It was reported that the fermentation process during tempe production improved the bioavailability of the protein, fiber, carbohydrates, isoflavones, vitamins [ 6 , 7 , 8 ]. These characteristics have made tempe a healthy protein source for plant-based diets, vegans, and vegetarians [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics-Based Study of the Effect of Raw Materials to the End Product of Tempe—An Indonesian Fermented Soybean

et al. 2020

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Tempe is a fermented soybean food from Indonesia, made by inoculating Rhizopus spp. onto cooked and dehulled soybean. Tempe has been a part of Indonesian culture since the 16th century and is now produced globally as a highly nutritious plant-based food. Despite a longstanding history on the production of tempe, very few studies have been reported to understand the effect of raw material to the end product metabolite composition. In this research, we applied GC/MS-based metabolite profiling to investigate the effect of various possible factors that might affect the final product (environmental factors, raw materials, and starter cultures). Representative samples were tempe produced by Indonesian industries, Japanese industries as well as laboratory made tempe. The results showed that both environmental factors and raw materials (soybean, water, and starter culture) contributed to the tempe metabolite profile. Here we found the possibility that starter cultures might play a greater role to determine the metabolite profiles compared to other tested factors. This research might provide useful insights for the larger scale industries to maintain the quality of tempe for the benefit of the consumers.

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Comparing the effect of tempe flour and tofu flour consumption on estrogen serum in ovariectomized rats

Cited by 9 publications

References 18 publications

Isoflavone and Antioxidant of Instant Cream Soup Made from Pumpkin and Tempeh and Their Active Compound in Ovariohysterectomy Rat-Induced Alzheimer’s Disease

Isoflavone and Antioxidant of Instant Cream Soup Made from Pumpkin and Tempeh and Their Active Compound in Ovariohysterectomy Rat-Induced Alzheimer’s Disease

Chinese medicine Bazi Bushen capsule improves lipid metabolism in ovariectomized female ApoE−/− mice

Metabolomics-Based Study of the Effect of Raw Materials to the End Product of Tempe—An Indonesian Fermented Soybean

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