“…Then, ten μl of CA at increasing concentrations (50, 100, 250, 500 and 750 μM; final concentrations), were incubated with 200 μl FRAP solution (3.1 mg sodium acetate plus 16 ml acetic acid, 40 mM HCl, 10 mM 2,4,6-Tri(2-pyridyl)-s-triazine (TPTZ) and 20 mM FeCl3). Optical density was recorded and processed, and the reduction capacity values were calculated according to a method previously described (Colonnello et al, 2018).…”