“…Recent research has mainly focused on pretreatment of straw to enhance biogas production, including pretreatment with extrusion , microbial consortia (Wen et al, 2015), enzymatic hydrolysis (Gao et al, 2012), steam explosion (Theuretzbacher et al, 2015), hydrothermal conditions using liquid hot water or steam (Gonzalez et al, 2014;He et al, 2014), ammonia (Yang et al, 2014), sodium hydroxide (Di Girolamo et al, 2014;Li et al, 2014), hydrochloric acid (Han et al, 2012), or sulfuric acid (Nasirian et al, 2011). Usually, microbial pretreatment consumes a carbon source, which expectedly is used as a substrate for methane production.…”