Comparison and interpretation of characteristics of Rhizosphere microbiomes of three blueberry varieties

Abstract

Background Studies on the rhizosphere microbiome of various plants proved that rhizosphere microbiota carries out various vital functions and can regulate the growth and improve the yield of plants. However, the rhizosphere microbiome of commercial blueberry was only reported by a few studies and remains elusive. Comparison and interpretation of the characteristics of the rhizosphere microbiome of blueberry are critical important to maintain its health. Results In this study, a total of 20 rhizosphere soil samples, including 15 rhizosphere soil samples from three different blueberry varieties and five bulk soil samples, were sequenced with a high-throughput sequencing strategy. Based on these sequencing datasets, we profiled the taxonomical, functional, and phenotypic compositions of rhizosphere microbial communities for three different blueberry varieties and compared our results with a previous study focused on the rhizosphere microbiome of blueberry varieties. Our results demonstrated significant differences in alpha diversity and beta diversity of rhizosphere microbial communities of different blueberry varieties and bulk soil. The distribution patterns of taxonomical, functional, and phenotypic compositions of rhizosphere microbiome differ across the blueberry varieties. The rhizosphere microbial communities of three different blueberry varieties could be distinctly separated, and 28 discriminative biomarkers were selected to distinguish these three blueberry varieties. Core rhizosphere microbiota for blueberry was identified, and it contained 201 OTUs, which were mainly affiliated with Proteobacteria, Actinobacteria, and Acidobacteria. Moreover, the interactions between OTUs of blueberry rhizosphere microbial communities were explored by a co-occurrence network of OTUs from an ecological perspective. Conclusions This pilot study explored the characteristics of blueberry’s rhizosphere microbial community, such as the beneficial microorganisms and core microbiome, and provided an integrative perspective on blueberry’s rhizosphere microbiome, which beneficial to blueberry health and production.

Root Niches of Blueberry Imprint Increasing Bacterial-Fungal Interkingdom Interactions along the Soil-Rhizosphere-Root Continuum

“…The varied compartment niches affected the proportions of the root-associated microbial community composition of blueberry. Consistent with previous studies, Actinobacteria and Proteobacteria were the most abundant phyla in the bacterial community ( 22 ), and our results showed that Actinobacteria was the dominant phylum in the endosphere. Members of Actinobacteria possess a potential suppressive effect on plant pathogens ( 53 ), and the increased proportion in the endosphere may have been related to the selection of functions that correspond to plant health requirements.…”

“…In addition, ericoid mycorrhizal (ERM) fungi can form symbiotic relationships with blueberry roots that can play a critical role in the survival of plants growing in habitats with low soil pH and slow organic matter turnover by secreting a broad range of enzymes, including cellulases, proteases, polyphenol oxidases, and phosphatases, which decompose complex organic compounds and enhance the fitness and nutrient acquisition of the host plant ( 18 – 20 ). Several studies have focused on the impact of host cultivars ( 21 – 23 ), differential habitats ( 16 , 24 , 25 ), and agricultural practices ( 26 ), but further exploration of the interactions of blueberry root-associated microorganisms along the soil-rhizosphere-root continuum is needed.…”

Oxytetracycline Increases the Residual Risk of Imidacloprid in Radish (Raphanus sativus) and Disturbs the Plant-Rhizosphere Microbiome Holobiont Homeostasis

“…For the collection of rhizosphere soil samples, refer to a prior article. 23 Specifically, the rhizosphere soil was quickly collected by gently brushing the remaining soil sticking on the roots (the rhizosphere soil was 3−5 mm above the roots) with brush pencils. Rhizosphere soil samples were collected in five parallels.…”

Growth Responses Of Maize And Soybean To Application Of Rhizosphere Bacterial Consortium From Dry Land North Lombok

“…Kelompok Bacillus, Pseudomonas, Aspergillus, Fusarium, dan Colletotrichum dapat ditemukan pada rhizosfer tanaman stoberi (Putra et al, 2020). Tingkat keanekaragaman yang berbeda juga ditemukan sampai pada tingkat varietas tumbuhan seperti yang ditemukan pada blueberry (Zhang et al, 2021).…”