Comparison and Standardization among Chlorophyll Meters in their Readings on Rice Leaves

Huang, Jikun; Peng, Shaobing

doi:10.1626/pps.7.97

Cited by 10 publications

(9 citation statements)

References 6 publications

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“…In our study, the SPAD-502 proved to be an effective tool for estimating total foliar concentration and content in plants of L. melissifolia across a range of plant ages, growing conditions, and genotypes, as well as, in the congener L. benzoin. However, different SPAD-502 meters may give different meter values (Huang & Peng 2004) and we suggest standardisation of a calibration curve prior to use on a plant population. Once this is determined, the SPAD-502 chlorophyll meter may be used to gauge the progress of conservation practices by assessing and monitoring physiological variables that reflect the relative health of threatened and endangered plant species.…”

Section: Discussionmentioning

confidence: 99%

Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research

Hawkins

Gardiner

Comer

2009

Journal for Nature Conservation

100

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Section: Discussionmentioning

confidence: 99%

Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research

Hawkins

Gardiner

Comer

2009

Journal for Nature Conservation

100

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“…On the other hand, a digital chlorophyll meter (Soil and Plant Analyzer Development [SPAD] meter) provides a non-destructive method for estimating leaf chlorophyll content by measuring light absorption of specific spectral bands in living leaves (Watanabe et al 1980;Chubachi et al 1986). The methods for measurement of SPAD values are simple and quick, and close correlations between SPAD values and leaf photosynthesis values have been observed in rice (Huang and Peng 2004;Kato et al 2004;Kumagai et al 2009). Therefore, SPAD measurement may be a more appropriate method than destructive measurement of leaf chlorophyll content for use in genetic analysis of leaf photosynthesis.…”

Section: Introductionmentioning

confidence: 86%

A Quantitative Trait Locus for Chlorophyll Content and its Association with Leaf Photosynthesis in Rice

Takai

Kondo

Yano

et al. 2010

Rice

118

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Leaf photosynthesis, an important determinant of yield potential in rice, can be estimated from measurements of chlorophyll content. We searched for quantitative trait loci (QTLs) for Soil and Plant Analyzer Development (SPAD) value, an index of leaf chlorophyll content, and assessed their association with leaf photosynthesis. QTL analysis derived from a cross between japonica cultivar Sasanishiki and high-yielding indica cultivar Habataki detected a QTL for SPAD value on chromosome 4. This QTL explained 31% of the total phenotypic variance, and the Habataki allele increased the SPAD value. Chromosomal segment substitution line (CSSL) with the corresponding segment from Habataki had a higher leaf photosynthetic rate and SPAD value than Sasanishiki, suggesting an association between SPAD value and leaf photosynthesis. The CSSL also had a lower specific leaf area (SLA) than Sasanishiki, reflecting its thicker leaves. Substitution mapping under Sasanishiki genetic background demonstrated that QTLs for SPAD value and SLA were co-localized in the 1,798-kb interval. The results suggest that the phenotypes for SPAD value and SLA are controlled by a single locus or two tightly linked loci, and may play an important role in increasing leaf photosynthesis by increasing chlorophyll content or leaf thickness, or both.

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“…A few studies reported differences in SPAD‐502 values acquired from different chlorophyll meters (Marquard and Tipton, 1987; Huang and Peng, 2004), although Schaper and Chacko (1991) did not find differences between their two chlorophyll meters. Marquard and Tipton (1987) suggested using Wratten filters for meter comparisons, similar to what was done in this study with the Roscolux plastic filters.…”

Section: Resultsmentioning

confidence: 99%

Chlorophyll Meter Calibrations for Chlorophyll Content Using Measured and Simulated Leaf Transmittances

Hunt

Daughtry

2014

Agronomy Journal

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Konica-Minolta SPAD-502 leaf chlorophyll meters provide a relative value of leaf chlorophyll content, and from the literature, there are considerable variations among the calibration equations between total chlorophyll contents (mg chlorophyll a + b cm -2 ) and SPAD-502 values. Our objective was to determine the leaf properties that contributed to the variations in calibration. We determined the internal calibration coe cient of ve SPAD-502 meters so that leaf transmittances in the red (650 nm) and near-infrared (940 nm) could be used to calculate SPAD-502 values. A leaf optics model, PROSPECT, was used to simulate transmittances and the chlorophyll-SPAD-502 relationship for di erent leaf optical properties. Spectral and leaf data from maize (Zea mays L.) showed that PROSPECT predicted leaf transmittances within 2%. Maize leaf data used in the PROSPECT model predicted the relationship between chlorophyll content and the SPAD-502 value, although a polynomial regression was a better t to the data. ere was a physical interaction between chlorophyll content and optical leaf structure a ecting leaf transmittances, which is not in the equation for calculating SPAD-502 values. Changing the PROSPECT leaf structure parameter resulted in di erent chlorophyll-SPAD-502 meter relationships, which were similar to the measured range of variation from calibration equations found in the literature. If the red and near-infrared transmittances are saved for each chlorophyll meter reading, then leaf radiative transfer models such as PROSPECT may be inverted to determine the actual leaf chlorophyll content. . Th e USDA is an equal opportunity provider and employer. Mention of trade names does not imply endorsement by the USDA.

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Comparison and Standardization among Chlorophyll Meters in their Readings on Rice Leaves

Cited by 10 publications

References 6 publications

Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research

Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research

A Quantitative Trait Locus for Chlorophyll Content and its Association with Leaf Photosynthesis in Rice

Chlorophyll Meter Calibrations for Chlorophyll Content Using Measured and Simulated Leaf Transmittances

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