Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research

Hawkins, Tracy S.; Gardiner, Emile S.; Comer, Greg S.

doi:10.1016/j.jnc.2008.12.007

Cited by 100 publications

(75 citation statements)

References 11 publications

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“…1 for the selected polynomial functions. Others have similarly reported that such polynomial functions best describe the relationship between SPAD values and chlorophyll concentration (Hawkins et al 2009;Markwell et al 1995).…”

Section: Resultsmentioning

confidence: 93%

Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana

2010

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The SPAD-502 meter is a hand-held device that is widely used for the rapid, accurate and non-destructive measurement of leaf chlorophyll concentrations. It has been employed extensively in both research and agricultural applications, with a range of different plant species. However, its utility has not been fully exploited in relation to the most intensively studied model organism for plant science research, Arabidopsis thaliana. Measurements with the SPAD-502 meter produce relative SPAD meter values that are proportional to the amount of chlorophyll present in the leaf. In order to convert these values into absolute units of chlorophyll concentration, calibration curves must be derived and utilized. Here, we present calibration equations for Arabidopsis that can be used to convert SPAD values into total chlorophyll per unit leaf area (nmol/cm 2 ; R 2 = 0.9960) or per unit fresh weight of leaf tissue (nmol/mg; R 2 = 0.9809). These relationships were derived using a series of Arabidopsis chloroplast biogenesis mutants that exhibit chlorophyll deficiencies of varying severity, and were verified by the subsequent analysis of senescent or light-stressed leaves. Our results revealed that the converted SPAD values differ from photometric measurements of solventextracted chlorophyll by just ~6% on average.

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Section: Resultsmentioning

confidence: 93%

Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana

2010

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“…In recent years, chlorophyll meters have offered a fast and convenient alternative for foliar Chls measurement. The Minolta SPAD 502 is used for a wide variety of leaf chlorophyll and nitrogen estimation by measuring the amount of light transmittance and absorptance in an easy and non-destructive way (Takebe et al, 1990;Ma et al, 1995;Blackmer and Schepers, 1995;Cate and Perkins, 2003;Read et al, 2003;Rowland et al, 2004;Hawkins et al, 2009;Rascher et al, 2009;Boegh et al, 2012). Nevertheless, it will be extremely hard work to extend the result of traditional methods to field study because such a single leaf measurement will be useless (due to no connection) for up-scaling the Chls and/or the fraction of absorbed photosynthetically active radiation (Gond et al, 1999) for the levels at tree crown or stand canopy.…”

Section: Indexmentioning

confidence: 99%

A novel reflectance-based model for evaluating chlorophyll concentrations of fresh and water-stressed leaves

et al. 2015

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Abstract. Water deficits can cause chlorophyll degradation which decreases the total concentration of chlorophyll a and b (Chls). Few studies have investigated the effectiveness of spectral indices under water-stressed conditions. Chlorophyll meters have been extensively used for a wide variety of leaf chlorophyll and nitrogen estimations. Since a chlorophyll meter works by sensing leaves absorptance and transmittance, the reading of chlorophyll concentration will be affected by changes in transmittance as if there were a water deficit in the leaves. The overall objective of this paper was to develop a novel and reliable reflectance-based model for estimating Chls of fresh and water-stressed leaves using the reflectance at the absorption bands of chlorophyll a and b and the red edge spectrum.Three independent experiments were designed to collect data from three leaf sample sets for the construction and validation of Chls estimation models. First, a reflectance experiment was conducted to collect foliar Chls and reflectance of leaves with varying water stress using the ASD FieldSpec spectroradiometer. Second, a chlorophyll meter (SPAD-502) experiment was carried out to collect foliar Chls and meter readings. These two data sets were separately used for developing reflectance-based or absorptance-based Chls estimation models using linear and nonlinear regression analysis. Suitable models were suggested mainly based on the coefficient of determination (R 2 ). Finally, an experiment was conducted to collect the third data set for the validation of Chls models using the root mean squared error (RMSE) and the mean absolute error (MAE). In all of the experiments, the observations (real values) of the foliar Chls were extracted from acetone solution and determined by using a Hitachi U-2000 spectrophotometer.The spectral indices in the form of reflectance ratio/difference/slope derived from the Chl b absorption bands (ρ 645 and ρ 455 ) provided Chls estimates with RMSE around 0.40-0.55 mg g −1 for both fresh and water-stressed samples. We improved Chls prediction accuracy by incorporating the reflectance at red edge position (ρ REP ) in regression models. An effective chlorophyll indicator with the form of (ρ 645 -ρ 455 ) / ρ REP proved to be the most accurate and stable predictor for foliar Chls concentration. This model was derived with an R 2 of 0.90 (P < 0.01) from the training samples and evaluated with RMSE 0.35 and 0.38 mg g −1 for the validation samples of fresh and water-stressed leaves, respectively. The average prediction error was within 14 % of the mean absolute error.

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“…An imbalance between biosynthesis and catabolic turnover of green pigments in plant tissues indicates profound inhibition of photosynthesis process (Heng-Moss et al, 2003;Smith et al, 2005;Botha et al, 2006;Diaz-Montano et al, 2007;Goławska et al, 2010). Portable leaf-clip-type chlorophyll meters perform rapid, repetitive and nondestructive estimates of Chl concentrations in living plants (Hawkins et al, 2009;Jinwen et al, 2009;Coste et al, 2010;Gholizadeh et al, 2011;Shrestha et al, 2012;Ghosh et al, 2013). The use of these reliable and advanced instruments for measurement of green pigment content is not restricted to controlled laboratory bioassays but can be extended to experiments in the plants' natural environment (Uddling et al, 2007;Ruiz-Espinoza et al, 2010;Neto et al, 2011;Cerovic et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

Sytykiewicz¹,

Czerniewicz²,

Sprawka³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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We measured the total chlorophyll (Chl a+b) content in seedling leaves of fifteen maize cultivars infested by two studied aphid species (oligophagous Rhopalosiphum padi L., monophagous Sitobion avenae F.) 7 and 14 days after the beginning of infestation, using a SPAD-502 chlorophyll meter. Chlorophyll loss was more severe in R. padi-infested than in S. avenae-infested plants. Chlorophyll depletion was greater after long-term (14 days) than after short-term aphid infestation in the investigated host systems. Seedlings of Złota Karłowa and Tasty Sweet were more damaged by aphid feeding; Ambrozja and Płomyk plants were less damaged by aphid feeding.K Ke ey y w wo or rd ds s: : Total chlorophyll (Chl a+b) content, SPAD units, maize, Rhopalosiphum padi, Sitobion avenae.

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Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research

Cited by 100 publications

References 11 publications

Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana

Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana

A novel reflectance-based model for evaluating chlorophyll concentrations of fresh and water-stressed leaves

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

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