Comparison and utilization of different PCR-based approaches for molecular typing ofPseudomonas syringaepv.actinidiaestrains from Italy

Abstract

To cite this Article Mazzaglia, Angelo , Renzi, Marsilio and Balestra, Giorgio M.( 2011) 'Comparison and utilization of different PCR-based approaches for molecular typing of Pseudomonas syringae pv. actinidiae strains from Italy',

Using fundamental knowledge of induced resistance to develop control strategies for bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae

“…However, the latest outbreak of Psa which started in Italy in 2008 and rapidly spread throughout most of the kiwifruit growing regions of the world, represents a major threat to the global kiwifruit industry (Vanneste, 2012). The pathovar actinidiae is not a genetically homogeneous pathovar; strains can be grouped in four biovars based on their molecular, microbiological and pathogenic characteristics (Vanneste et al, 2013) which is consistent with MLST and whole genome sequence analysis (Ferrante and Scortichini, 2010; Mazzaglia et al, 2011; Chapman et al, 2012). The recent outbreak of bacterial canker on kiwifruit in Europe and New Zealand is caused by the same biovar of Psa (biovar 3) (Chapman et al, 2012; Vanneste et al, 2013).…”

PM7/120 (1)Pseudomonas syringaepv. actinidiae

“…Repetitive PCR (rep‐PCR) has been shown to be highly reliable for strain identification of pseudomonads, xanthomonads and other bacteria at the species and infraspecies level (Louws et al ., ; Rademaker & DeBruijn, ) by means of amplification of interspersed repetitive DNA sequences present in bacterial genomes. One or more primers (REP, BOX, ERIC) might be used during this genotyping technique (Ferrante & Scortichini, ; Mazzaglia et al ., ). Guidance on how to perform rep‐PCR tests for the identification of bacterial isolates is given in PM 7/100 Rep‐PCR tests for identification of bacteria .…”

Survival of <i>Pseudomonas syringae</i> pv <i>actinidiae</i> in nonkiwifruit green compost

“…Since 2010, Psa has been isolated from France (Vanneste et al 2011b), Portugal (Balestra et al 2010), Spain (Abelleira et al 2011), Turkey (Bastas & Karakaya 2011), Chile (EPPO 2011a), Switzerland (EPPO 2011b), Australia (EPPO 2011c) and New Zealand (Everett et al 2011;Vanneste et al 2011a). The strains isolated from those recent outbreaks are different from the strains isolated earlier (Ferrante & Scortichini 2010;Mazzaglia et al 2011;Chapman et al 2012;Vanneste et al 2013). Strains of Psa are now grouped in four categories called biovars (Vanneste et al 2013).…”