Comparison between arterial and venous sampling of circulating hormones, substrates and peptides in severe obesity

Abstract

Purpose: Severely obese patients are being encountered more frequently in clinical practice. Factors implicated in the relationship between obesity and cardiovascular disease may be measured from a blood sample obtained through arterial access in a cardiology setting, such as during cardiac catheterization or heart surgery. The comparability of a given sample site (arterial vs. venous) with regards to blood parameters is yet to be established. Methods: Fifteen severely obese patients undergoing bariatric surgery were recruited. Fasting blood samples were collected simultaneously from the radial artery (A) and the superior vena cava (V), both representing general circulating levels, after anesthesia but before the surgical procedure. Blood samples were analysed for glucose, insulin, non-esterified fatty acids (NEFA), leptin, adiponectin, total ghrelin, high sensitive C-reactive protein (hs-CRP) and amino-terminal pro-brain natriuretic peptide (NT-proBNP) concentrations. Results: Arterial and venous concentrations of all factors analysed showed no statistical difference (all p values > 0.1); leptin A: 39 ± 16 vs. V: 42 ± 18 ng/mL; total ghrelin A : 0.86 ± 0.27 vs. V : 0.76 ± 0.35 ng/mL; adiponectin A: 7.7 ± 3.3 vs. V: 7.7 ± 3.6 μg/mL; insulin A: 17.9 ± 9.7 vs. V: 18.6 ± 10.5 μU/mL; glucose A: 8.3 ± 2.1 vs. V: 7.9 ± 2.2 mM; NEFA A: 0.98 ± 0.93 vs. V: 0.89 ± 0.38 mM ; hs-CRP A: 10.17 ± 7.68 vs. V: 10.27 ± 7.30 μg/mL and NT-proBNP A: 54.3 ± 47.9 vs. V: 54.7 ± 49.3 pg/mL. Conclusion: These results suggest that radial artery and superior vena cava blood collection sites are comparable and may be used clinically with respect to fasting glucose, NEFA, leptin, adiponectin, total ghrelin, hs-CRP and NT-proBNP concentrations in a group of severely obese patients.

Interindividual Responses of Appetite to Acute Exercise

“…Recent studies suggest that compared to arterialized blood, venous blood provides lower concentrations of glucagonlike peptide-1 (38) as well as lower glucose concentrations and higher insulin sensitivity (39). Although limited evidence in patient populations suggests that fasting ghrelin concentrations are comparable between venous and arterialized blood (40,41), direct comparisons of acylated ghrelin and total PYY between arterialized and venous blood after exercise has not been investigated. Nevertheless, the findings of the present study are relevant to the wider exercise and appetite regulation literature where blood sampling from an antecubital vein is commonplace for quantifying appetiteregulatory hormone concentrations.…”

The ethnicity-specific association of biomarkers with the angiographic severity of coronary artery disease

“…Biomarker levels in our study were measured in arterial blood; these levels may differ from venous levels. However, Martin et al showed that arterial and venous levels of hsCRP and NTproBNP did not differ [ 35 ]. Also, levels of NTproBNP, hsCRP, CysC and MPO were measured using an in-house ELISA method instead of clinically standardised assays.…”

Reference values for N-terminal Pro-brain natriuretic peptide in premature infants during their first weeks of life

“…Arterial, central venous, peripheral venous, or capillary blood samples were analyzed on the Cobas E801 analyzer using the Elecsys proBNP II method (Roche Diagnostics). Different types of blood sampling presumably lead to comparable NT-proBNP values [ 35 , 36 ]. The measuring range for the used method is 5–35,000 ng/l.…”