Comparison between Effects of Cyclic AMP, Dibutyryl Cyclic AMP and Luteinizing Hormone on Ovarian Glycolysis

Herlitz, Hans; Hamberger, Lars; Ahrén, Kurt

doi:10.1111/j.1748-1716.1974.tb05735.x

Cited by 8 publications

(2 citation statements)

References 37 publications

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“…It is possible that in another endocrine organ, the ovary, glycolysis also would be stimulated by its respective tropic hormone, FSH. Interestingly, gonadotropins and dibutyrylcAMP rapidly stimulated glycolysis in prepubertal rat ovaries incubated in vitro [49].…”

Section: Discussionmentioning

confidence: 99%

Dual Actions of Phorbol Ester on Cytochrome P450 Cholesterol Side-Chain Cleavage Messenger Ribonucleic Acid Accumulation in Porcine Granulosa Cells1

Lahav¹,

Garmey²,

Shupnik³

et al. 1995

Biology of Reproduction

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In earlier studies in cultures of porcine granulosa cells prepared from small antral follicles, steroidogenesis-related loci were inhibited by treatment for 48 h with 12-O-tetradecanoyl-phorbol-13-acetate (TPA), a potent activator of protein kinase C (PKC). In the present investigation, cells were incubated in serum-free medium for 48 h, with various agents present during the last 2-24 h. With TPA at 30 ng/ml, the FSH-stimulated cAMP accumulation was markedly enhanced at all time points. FSH increased the concentration of cytochrome P450 cholesterol side-chain cleavage (P450scc) mRNA throughout the 24-h incubation. At 4 and 8 h, TPA increased the accumulation of P450scc mRNA, having an additive effect with FSH. However, at 24 h, TPA markedly suppressed the FSH-induced increased in P450scc mRNA. Pretreatment of cells with FSH did not shorten the time required for TPA to become inhibitory. The stimulatory effect of 8-bromo-cAMP on P450scc mRNA also was augmented by TPA at 4 h, but significant inhibition was not observed at 24 h. The concentration of glyceraldehyde-3-phosphate dehydrogenase mRNA, intended to be used for correction of gel loading, was stably increased by both cAMP and TPA. These effects of TPA suggest multiple actions of PKC(s) on the regulation of P450scc expression and other endpoints in ovarian granulosa cells.

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Section: Discussionmentioning

confidence: 99%

Dual Actions of Phorbol Ester on Cytochrome P450 Cholesterol Side-Chain Cleavage Messenger Ribonucleic Acid Accumulation in Porcine Granulosa Cells1

Lahav¹,

Garmey²,

Shupnik³

et al. 1995

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…Pregnenolone, testosterone, oestradiol-17ß and also dbcAMP-unlike gonadotrophins-all stimulate growth in the same limited mouse follicle population as does lithium lactate (Kent, 1973 ;Ryle, 1974). Since cyclic AMP rapidly enhances lactate production by incubated rat ovaries (Herlitz, Hamberger & Ahrén, 1974), it is possible that its growth-stimulating effects on cultured mouse ovaries, and also those of the three steroids, are mediated by this metabolic product. …”

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confidence: 99%