Comparison between HPLC and HPTLC-densitometry for the determination of harpagoside from Harpagophytum procumbens CO2-extracts

Devil's Claw—A review of the ethnobotany, phytochemistry and biological activity of Harpagophytum procumbens

“…The ratio is below 10 in H. procumbens and above 31 in H. zeyheri (Schmidt, 2005). Gunther and Schmidt (2005) developed high performance liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) methods for the quantification of harpagoside. The secondary tubers of H. procumbens were used to prepare 15 different carbon dioxide (CO 2 ) extracts.…”

Application of microemulsion thin layer chromatography for the fingerprinting of licorice (Glycyrrhiza spp.)

“…On the other hand, unless these parameters are carefully controlled and the analytical protocols well standardized, results in TLC analysis are often difficult to reproduce [4,5]. Although the reproducibility of TLC techniques has been improved significantly in recent years through the application of high-performance techniques (HPTLC) [6][7][8], there remains the need to develop better and standardized TLC method in real world applications [4,5,9,10]. In the past, inadequate reproducibility and relatively low resolution have been the two major factors hinder the widespread use of TLC in the analysis or quality control of herbal materials on a routine basis.…”

Quantitative High Performance Thin Layer Chromatography for the Analysis of Herbal Medicines: Problems and Advantages

“…Due to its simplicity, speed, and relatively low cost, as number of samples can be analysed simultaneously on the same plate using a small amount of solvent as the mobile phase Thin Layer Chromatography (TLC) has been mostly used for the qualitative analysis of herbal medicines and to help characterise and track components visually or as an initial separation technique [8,9]. As it has been unable to provide accurate quantitative data TLC has become less favoured as quantification method of analysis [6,10].…”