Comparison between Image and Flow DNA Cytometry in Non-Hodgkin's Lymphomas

Felman, Pascale; French, M.; Souchier, Catherine; Magaud, Jean Pierre; Gentilhomme, O; Bryon, Paul‐André

doi:10.1016/s0344-0338(89)80223-7

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Plasma cells were immunolabelled and were selected from the other bone marrow myeloid cells. In previous image analysis work, cell populations were more usually selected according to size, shape, or visual criteria (2). Image and flow cytometry DNA results are known to be similar except in these cases, when some cells are excluded from the image analysis.…”

Section: Discussionmentioning

confidence: 99%

Methods for cell proliferation analysis by fluorescent image cytometry

et al. 1995

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Methods were developed for multimodal microscopic image analysis in order to identify and analyze one cell type under various microscopic conditions. Our purpose was to quantify both total DNA content using propidium iodide (PI) stain and S-phase fraction using the bromodeoxyuridine (BrdUrd) incorporation technique in cell population subsets. The model chosen was plasma cells in bone marrow triply labelled with fluorescein isothiocyanate (FITC) for intracytoplasmic immunoglobulins, with amino-methylcoumarin-acetate

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Section: Discussionmentioning

confidence: 99%

Methods for cell proliferation analysis by fluorescent image cytometry

et al. 1995

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“…Moreover, DNA content may be heterogeneous within discrete areas of a tumor (7,8,2 1,38), and in premalignant lesions DNA aneuploidy may be present in specific topographic regions of the tissue (own unpublished observations), which stresses the necessity to quantify DNA ploidy in the context of tissue architecture. DNA image cytometry (ICM), which permits morphological selection of cells, has been shown to be particularly useful for detection of rare event cells and polyploid populations (14). The use of this method in cytological preparations (smears, imprints) is widely accepted.…”

mentioning

confidence: 99%

Confocal 3-dimensional DNA image cytometry in thick tissue sections

et al. 1996

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We present a three‐dimensional confocal DNA image cytometry (3‐D CICM) method for analysis of DNA content in 30–40‐μm‐thick sections of routinely processed paraffin‐embedded specimens. A comparison of DNA ploidy profiles obtained by 3‐D CICM and conventional DNA image cytometry (ICM) on tissue sections showed significantly higher numbers of cells with high DNA content in DNA histograms by 3‐D CICM. As estimated by 3‐D CICM, the size of nuclei frequently exceeded the thickness of tissue sections used in conventional ICM, which suggested that many nuclei measured by this technique may be incomplete. This artifact was excluded in 3‐D CICM by automatic rejection of cut nuclear profiles. This and the favorable ratio of tissue thickness to nuclear size 3‐D CICM permitted the DNA quantitation even in large cells with highly increased DNA ploidy values such as megakaryocytes and Reed‐Stenberg cells of Hodgkin's disease. Additionally, 3D‐CICM allowed evaluation of morphometric parameters and 3‐dimensional reconstruction of studied cells. © 1996 Wiley‐Liss, Inc.

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“…The advantage of DNA image analysis is that the measured nuclei can be visualized at the time of measurement. Some authors suggest that with a limited number of nuclei reliable data can be obtained (132)(133)(134)(135)(136). When flow cytometry is used on deparaffinized tissue, internal controls are absent, and diploid peaks may show large CV's.…”

Section: Image Cytometrymentioning

confidence: 99%

“…After DNA staining by the Feulgen method and morphologic selection of specific nuclei the sum of the optical density of each nucleus is used to calculate the amount of DNA present. By this method a reliable estimation of DNA content has been demonstrated for several tumors including one study on non-Hodgkin lymphoma (132). So far, no data on Hodgkin's disease have been reported.…”

Section: Genera/aspecfsmentioning

confidence: 99%

Hodgkin's disease : clinical and biological determinants of prognosis

Erdkamp¹

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show abstract

Comparison between Image and Flow DNA Cytometry in Non-Hodgkin's Lymphomas

Cited by 7 publications

References 14 publications

Methods for cell proliferation analysis by fluorescent image cytometry

Methods for cell proliferation analysis by fluorescent image cytometry

Confocal 3-dimensional DNA image cytometry in thick tissue sections

Hodgkin's disease : clinical and biological determinants of prognosis

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