Comparison between skin-sensitizing antibody titers and leukocyte sensitivity measurements as an index of the severity of ragweed hay fever

Lichtenstein, Lawrence M.; Norman, Philip S.; Connell, John T.

doi:10.1016/0021-8707(67)90005-6

Cited by 36 publications

(23 citation statements)

References 13 publications

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“…In accordance with their reduced titers of Ascaris-specific IgE, NHP treated with Ab01 or Ab02 did not undergo the increase in basophil histamine release that accompanied Ascaris challenge in control animals. In human subjects, basophil histamine release has proved to be a useful correlate of allergen sensitivity, showing associations with skin test reactivity, specific IgE, and symptom score (Lichtenstein et al, 1967;Shim et al, 2003). IgE neutralization using a humanized antibody (omalizumab; Xolair) has proved to be an effective therapeutic strategy for asthma (Fahy, 2006), leading to reductions in circulating IgE titer, FcεRI expression, and basophil sensitivity to allergen (MacGlashan et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Interleukin-13 Neutralization by Two Distinct Receptor Blocking Mechanisms Reduces Immunoglobulin E Responses and Lung Inflammation in Cynomolgus Monkeys

Kasaian

Tan²,

Jin³

et al. 2008

J Pharmacol Exp Ther

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Interleukin (IL)-13 is a key cytokine driving allergic and asthmatic responses and contributes to airway inflammation in cynomolgus monkeys after segmental challenge with Ascaris suum antigen. IL-13 bioactivity is mediated by a heterodimeric receptor (IL-13R␣1/IL-4R␣) and can be inhibited in vitro by targeting IL-13 interaction with either chain. However, in cytokine systems, in vitro neutralization activity may not always predict inhibitory function in vivo. To address the efficacy of two different IL-13 neutralization mechanisms in a primate model of atopic disease, two humanized monoclonal antibodies to IL-13 were generated, with highly homologous properties, differing in epitope recognition. Ab01 blocks IL-13 interaction with IL-4R␣, and Ab02 blocks IL-13 interaction with IL-13R␣1. In a cynomolgus monkey model of IgE responses to A. suum antigen, both Ab01 and Ab02 effectively reduced serum titers of Ascaris-specific IgE and diminished ex vivo Ascaris-triggered basophil histamine release, assayed 8 weeks after a single administration of antibody. The two antibodies also produced comparable reductions in pulmonary inflammation after lung segmental challenge with Ascaris antigen. Increased serum levels of IL-13, lacking demonstrable biological activity, were seen postchallenge in animals given either anti-IL-13 antibody but not in control animals given human IgG of irrelevant specificity. These findings demonstrate a potent effect of IL-13 neutralization on IgE-mediated atopic responses in a primate system and show that IL-13 can be efficiently neutralized by targeting either the IL-4R␣-binding epitope or the IL-13R␣1-binding epitope.

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Section: Discussionmentioning

confidence: 99%

Interleukin-13 Neutralization by Two Distinct Receptor Blocking Mechanisms Reduces Immunoglobulin E Responses and Lung Inflammation in Cynomolgus Monkeys

Kasaian

Tan²,

Jin³

et al. 2008

J Pharmacol Exp Ther

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“…Because mast cells are found in almost all tissues except blood, where they seemed to be replaced by basophils, Riley and West suggested that these cells may be involved in anaphylaxis. Somewhat later, the Hopkins's school in Baltimore led by Philip Norman and Lawrence Lichtenstein showed in a comparative study of PK-sensitizing antibody titers and leukocyte sensitivity measurements that the latter represented a valuable index of the severity of ragweed hay fever (28). After the discovery of IgE, the Ishizakas also found IgE binding sites present on basophils and monkey mast cells and that their aggregation by anti-IgE on basophils caused them to release histamine (29,30).…”

Section: The Cellular Playersmentioning

confidence: 99%

The history of mast cell and basophil research – some lessons learnt from the last century

Blank

Falcone

Nilsson

2013

Allergy

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“…On the other hand, the development of HRT in the 1960s [18] did not lead to its widespread use outside of research laboratories due to the large volume of blood required and the short testing window after blood sampling [19]. In recent years, a novel HRT method has been developed that is more convenient and requires a smaller volume of blood.…”

Section: Introductionmentioning

confidence: 99%

Utility of the Peripheral Blood Basophil Histamine Release Test in the Diagnosis of Hen’s Egg, Cow’s Milk, and Wheat Allergy in Children

Sato

Tachimoto²,

Shukuya³

et al. 2011

Int Arch Allergy Immunol

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Background: The diagnosis of food allergy (FA) is made by oral food challenge tests (OFCs) that occasionally produce serious symptoms in patients; therefore, whether to perform OFCs should be carefully considered. The utility of the histamine release test (HRT) in the diagnosis of childhood FA has not been fully examined. Methods: Sixty-four subjects with suspected hen’s egg allergy, cow’s milk allergy (CMA), and wheat allergy (WA) were enrolled. The diagnosis of FA was made based on the outcomes of OFCs or a convincing history of symptoms after food ingestion within 6 months before or after sample collection. HRT was performed using an HRT Shionogi kit. The threshold of histamine release (HRT threshold), which was defined as the minimum concentration of food antigen to induce a 10% net histamine release, was analyzed in association with FA diagnosis. Results: Receiver operating characteristic analysis showed that the HRT threshold was useful in the diagnosis of heated egg allergy (HEA), raw egg allergy (REA), CMA, and WA. We were able to determine the cutoff value for the HRT threshold in relation to outcomes of OFCs. The cutoff value was 6 ng/ml of egg white antigen in HEA and REA (p < 0.01), 40 ng/ml of milk antigen in CMA (p < 0.01), and 500 ng/ml of wheat antigen in WA (p < 0.05). The efficiency was 70.3% for HEA, 78.0% for REA, 77.6% for CMA, and 70.7% for WA. Conclusions: We conclude that the HRT threshold measurement for egg white, milk, and wheat antigen is related to outcomes of OFCs and is useful in determining when OFCs should be performed.

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Comparison between skin-sensitizing antibody titers and leukocyte sensitivity measurements as an index of the severity of ragweed hay fever

Cited by 36 publications

References 13 publications

Interleukin-13 Neutralization by Two Distinct Receptor Blocking Mechanisms Reduces Immunoglobulin E Responses and Lung Inflammation in Cynomolgus Monkeys

Interleukin-13 Neutralization by Two Distinct Receptor Blocking Mechanisms Reduces Immunoglobulin E Responses and Lung Inflammation in Cynomolgus Monkeys

The history of mast cell and basophil research – some lessons learnt from the last century

Utility of the Peripheral Blood Basophil Histamine Release Test in the Diagnosis of Hen’s Egg, Cow’s Milk, and Wheat Allergy in Children

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