Comparison between strains of infectious bovine rhinotracheitis virus (Bovid herpesvirus 1), from respiratory and genital origins, using polyacrylamide gel electrophoresis of structural proteins

Pastoret, Paul-Pierre; Burtonboy, Guy; Aguilar-Setién, Álvaro; Godart, Marie-Françoise; Lamy, M; Schoenaers, Frédéric

doi:10.1016/0378-1135(80)90004-8

Cited by 29 publications

(16 citation statements)

References 18 publications

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“…The protein we have designated VP8 (Misra et al, 1981) is made in the later stages of infection and is the most abundant of the virion proteins of BHV-1 (Misra et al, 1981;Pastoret et al, 1980;Marshall et al, 1986). VP8 has also been shown to reside in the viral tegument (Marshall et al, 1986) and G. Weinmaster (unpublished results) demonstrated that BHV-l-infected cells contain a phosphoprotein with an electrophoretic mobility similar to that of VP8.…”

Section: Vp8 Is a Y2 Phosphoproteinmentioning

confidence: 99%

“…Virions of bovine herpesvirus type 1 (BHV-1) comprise about 25 to 33 polypeptides (Pastoret et al, 1980;Misra et al, 1981). Cells infected with the virus contain an additional 15 polypeptides that are not represented in purified virions (Misra et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

“…Virions of BHV-1 contain an abundance of a protein that has been designated VP8 (Misra et al, 1981), VP7 (Pastoret et al, 1980) or 107K (Marshall et al, 1986). The protein, which is also the most abundant viral protein in infected cells, has been shown to be located in the tegument of virions (Marshall et al, 1986) and preliminary results (G. Weinmaster, unpublished results) suggest that it is a 7 phosphoprotein.…”

Section: Introductionmentioning

confidence: 99%

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The Most Abundant Protein in Bovine Herpes 1 Virions is a Homologue of Herpes Simplex Virus Type 1 UL47

Carpenter

Misra

1991

Journal of General Virology

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The bovine herpesvirus type 1 (BHV-1) protein VP8 is present, in large amounts, in the tegument of virions. As a preliminary step towards determining the function of VP8 and the biological relevance for its abundant presence, we describe the mapping of the location of its gene and determination of its nucleotide sequence. The gene for VP8 was located between 0.088 and 0.108 map units on the BHV-1 genome and contained a 2226 bp reading frame encoding a 742 amino acid protein.The protein, produced in vitro by transcribing and translating the reading frame, was precipitated by monoclonal antibodies and polyclonal serum directed against VP8. The primary structure of VP8 showed considerable homology with the product of the UL47 reading frame of herpes simplex virus type 1.

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Section: Vp8 Is a Y2 Phosphoproteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Most Abundant Protein in Bovine Herpes 1 Virions is a Homologue of Herpes Simplex Virus Type 1 UL47

Carpenter

Misra

1991

Journal of General Virology

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“…The Ishikawa-B strain and the Ishikawa-S strain were isolated from a cow and a Japanese serow with papular stomatitis, respectively, and kindly supplied by the Nanbu Livestock Hygiene Service Center in Ishikawa Prefecture. Vaccinia virus (Lc16mO strain), bovine herpesvirus type 1 (Los Angeles strain) and bovine viral diarrhea virus (Nose strain) were preserved in the National Institute of Animal Health [4,5,15]. All viruses were propagated in fetal bovine muscle (FBM) cells.…”

mentioning

confidence: 99%

An Epidemic of Parapoxvirus Infection among Cattle: Isolation and Antibody Survey.

Kuroda

Yoshida

Shibahara

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. A disease characterized by papules, nodules, vesicles and, rarely, pustules and ulcers on teats was seen among cattle on a farm in Chiba Prefecture, Japan. A virus was isolated by inoculation of fetal bovine lung cell cultures from a vesicle on a teat of an infected cow. The virus was subsequently passaged in fetal bovine lung and muscle cells in which it produced complete cytopathic changes. The virus was identified by physicochemical examinations and electromicroscopic observation as a parapoxvirus. A seroepidemiological survey was performed on antibody to the isolated virus by the agar gel immunodiffusion test. The isolated virus formed a precipitation line which cross reacted with other parapoxviruses isolated previously in Japan. The positive rate was more than 50% among cattle in the Kanto district. The positive rate increased with age. It was suggested that parapoxvirus infection might have already been prevalent among cattle in Japan.-KEY WORDS: cattle, dermatitis, mammilitis, papula, parapoxvirus.J. Vet. Med. Sci. 61 (7): [749][750][751][752][753] 1999 48 hr and fresh medium was added. The cells were cultivated again for at least 12 days. The inoculated cell cultures were freeze-thawed three times and inoculated onto fresh FBL cells if a cytopathic effect (CPE) was not observed. The blind passage was repeated twice.Control viruses: Four strains of parapoxvirus isolated previously in Japan and vaccinia virus were used as controls for serological tests. The Aomori and Iwate strains were isolated from a cow with bovine papular stomatitis and a sheep with contagious pustular dermatitis, respectively, and preserved in the National Institute of Animal Health [7]. The Ishikawa-B strain and the Ishikawa-S strain were isolated from a cow and a Japanese serow with papular stomatitis, respectively, and kindly supplied by the Nanbu Livestock Hygiene Service Center in Ishikawa Prefecture. Vaccinia virus (Lc16mO strain), bovine herpesvirus type 1 (Los Angeles strain) and bovine viral diarrhea virus (Nose strain) were preserved in the National Institute of Animal Health [4,5,15]. All viruses were propagated in fetal bovine muscle (FBM) cells.Cell cultures: FBL cell cultures and FBM cell cultures were prepared by an original tissue-culture method. FBL cells were used within 5 passages for virus isolation and FBM cells were within 20 passages for physicochemical examinations of the isolated virus and viral antigen preparation. MDBK cells were employed for antigen preparation of the isolated virus for the agarose gel immunodiffusion (AGID) test. These cells were cultivated at 37°C in Eagle's MEM containing 5% fetal calf serum and 0.3% tryptose phosphate broth.Electron microscopic observation: Culture fluids collected from FBM cells infected with the isolated virus and induced CPE were centrifuged for 30 min at 10,000 g. The supernatant was then centrifuged for 2 hr at 110,000 g.

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“…Other animal DNA viruses used in this study are: a Los Angeles strain of bovine herpesvirus 1 (BoHV-1) [11], a WC-11 strain of alcelaphine herpesvirus 1 (AlHV-1) [12], ovine herpesvirus 2 (OvHV-2) [13], an HH-1 strain of equine herpesvirus 1 (EHV-1) [8], a YS-81 strain of pseudorabies virus (PRV) [2], and a Fukuroi strain of bovine adenovirus 7 (BAdV-7) [7]. Additionally, DNA samples extracted from a Chiba strain of parapoxvirus (PPV) [9] and a B11-41 strain of bovine herpesvirus 4 (BoHV-4) [15] were provided by Dr. H. Sentsui, National Institute of Animal Health, Japan.…”

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confidence: 99%

Development of a Shuttle Polymerase Chain Reaction for the Detection of Bovine Herpesvirus 2.

Imai

Ishihara

Jayawardane

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. Three different polymerase chain reaction (PCR) protocols were evaluated for their ability to detect bovine herpesvirus 2 (BoHV-2): single-step PCR with 3 reaction stages (denaturation, annealing and extension), 2 reaction stages (denaturation and annealing/ extension; shuttle PCR), and semi-nested PCR with 3 reaction stages. All the PCR protocols showed the same sensitivity (detection limit of 0.4 TCID 50 ). A non-specific band sometimes appeared in mock cell DNA at annealing temperatures below 64°C. The shuttle PCR was found to be superior to the other protocols under consideration because of the speed of its application. Furthermore, no non-specific band was detected in DNAs of eight other DNA viruses. Thus, the shuttle PCR seems to be an excellent diagnostic tool for BoHV-2 infections. KEY WORDS: bovine herpesvirus 2 (BoHV-2), PCR.J. Vet. Med. Sci. 64(10): 953-956, 2002

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Comparison between strains of infectious bovine rhinotracheitis virus (Bovid herpesvirus 1), from respiratory and genital origins, using polyacrylamide gel electrophoresis of structural proteins

Cited by 29 publications

References 18 publications

The Most Abundant Protein in Bovine Herpes 1 Virions is a Homologue of Herpes Simplex Virus Type 1 UL47

The Most Abundant Protein in Bovine Herpes 1 Virions is a Homologue of Herpes Simplex Virus Type 1 UL47

An Epidemic of Parapoxvirus Infection among Cattle: Isolation and Antibody Survey.

Development of a Shuttle Polymerase Chain Reaction for the Detection of Bovine Herpesvirus 2.

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