Comparison between the Gene Expression Profile of Human Müller Cells and Two Spontaneous Müller Cell Lines

Lupien, C.; Bolduc, Carl; Landreville, Solange; Salesse, Christian

doi:10.1167/iovs.07-0122

Cited by 11 publications

(9 citation statements)

References 95 publications

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“…Recent evidence indicates that the glial and neural cells of the retina are also affected 24. This notion is consistent with our data, in that we observed several genes related to neurogenesis such as MTCH1 , SOD1 , S100A6 and BAIAP2 in the library, which is indicative of immature glial cells.…”

Section: Discussionsupporting

confidence: 93%

Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy

Ogura

Ishikawa

Yoshida

et al. 2009

British Journal of Ophthalmology

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Section: Discussionsupporting

confidence: 93%

Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy

Ogura

Ishikawa

Yoshida

et al. 2009

British Journal of Ophthalmology

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“…During the onset and progression of diseases, extensive changes take place in gene expression [17]. By comparing gene expression profiles under different conditions, individual genes or group of genes that play important roles in a particular disease process can be identified.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Gene Expression Profile of Epiretinal Membranes Obtained from Eyes with Proliferative Vitreoretinopathy to That of Secondary Epiretinal Membranes

et al. 2013

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BackgroundProliferative vitreoretinopathy (PVR) is a destructive complication of retinal detachment and vitreoretinal surgery which can lead to severe vision reduction by tractional retinal detachments. The purpose of this study was to determine the gene expression profile of epiretinal membranes (ERMs) associated with a PVR (PVR-ERM) and to compare it to the expression profile of less-aggressive secondary ERMs.Methodology/Principal FindingsA PCR-amplified complementary DNA (cDNA) library was constructed using the RNAs isolated from ERMs obtained during vitrectomy. The sequence from the 5′ end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs). We obtained 1116 nonredundant clusters representing individual genes expressed in PVR-ERMs, and 799 clusters representing the genes expressed in secondary ERMs. The transcriptome of the PVR-ERMs was subdivided by functional subsets of genes related to metabolism, cell adhesion, cytoskeleton, signaling, and other functions, by FatiGo analysis. The genes highly expressed in PVR-ERMs were compared to those expressed in the secondary ERMs, and these were subdivided by cell adhesion, proliferation, and other functions. Querying 10 cell adhesion-related genes against the STRING database yielded 70 possible physical relationships to other genes/proteins, which included an additional 60 genes that were not detected in the PVR-ERM library. Of these, soluble CD44 and soluble vascular cellular adhesion molecule-1 were significantly increased in the vitreous of patients with PVR.Conclusions/SignificanceOur results support an earlier hypothesis that a PVR-ERM, even from genomic points of view, is an aberrant form of wound healing response. Genes preferentially expressed in PVR-ERMs may play an important role in the progression of PVR and could be served as therapeutic targets.

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“… Key for references:1=Al-Ubaidi et al 2008; 2= Bonilha et al 2004; 3= Dean et al1999; 4= Diehn et al 2005; 5=Ferrington et al 2008; 6=Fimbel et al 2007; 7=Fokina and Frolova 2006; 8=Hargrave 2001; 9=Hauck et al 2003; 10=Helle et al 2003; 11=Huang et al 2004; 12=Imamura et al 2006; 13=Ivanov et al 2006; 14=Lewis et al 1989; 15=Lupien et al 2007; 16=Moscona et al 1970; 17=Saari 2000; 18=Tsai 2006; 19=West et al 2001; 20=Whelan and McGinnis 1988; 21= the present study. …”

Section: Figures and Tablesmentioning

confidence: 50%

“…A literature review revealed that 17 of the proteins that were differentially expressed under our experimental conditions are known to be expressed by and play a functional role in the eye (see Table 1 for expression patterns in various cell types). Those proteins are: HSPA8 (Al-Ubaidi et al 2008; Dean et al 1999), HSPD1 (Al-Ubaidi et al 2008; West et al 2001), ATP5B (Al-Ubaidi et al 2008; Lupien et al 2007; West et al 2001), PCNA (Fimbel et al 2007), RLBP1 (Saari 2000), GNB1 (Al-Ubaidi et al 2008; Lupien et al 2007; Whelan and McGinnis 1988), YWHAZ (Ivanov et al 2006) (West et al 2001), PSMA1 (Ferrington et al 2008), NDUFS3 (Huang et al 2004; Lupien et al 2007), WNT2 (Fokina and Frolova 2006), LYZ (Bonilha et al 2004; Tsai et al 2006), RBP1 (Saari 2000), FABP7 (Hauck et al 2003; Helle et al 2003), SOD1 (Diehn et al 2005; Huang et al 2004; Imamura et al 2006) (Lupien et al 2007), RHO (Hargrave 2001), CRKRS (http://www.dsi.univ-paris5.fr/genatlas/fiche.php?n=30221), GLUL (Moscona et al 1970), and GFAP (Lewis et al 1989). It is striking that Müller cells rather than photoreceptors express the vast majority of differentially expressed proteins, 14 of 17 and 6 of 17, respectively.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of the retina: Removal of RPE alters outer segment assembly and retinal protein expression

Wang¹,

Nookala²,

Narayanan³

et al. 2008

Glia

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The mechanisms that regulate the complex physiologic task of photoreceptor outer segment assembly remain an enigma. One limiting factor in revealing the mechanism(s) by which this process is modulated is that not all of the role players that participate in this process are known. The purpose of this study was to determine some of the retinal proteins that likely play a critical role in regulating photoreceptor outer segment assembly. To do so, we analyzed and compared the proteome map of tadpole Xenopus laevis retinal pigment epithelium (RPE)-supported retinas containing organized outer segments with that of RPE-deprived retinas containing disorganized outer segments. Solubilized proteins were labeled with CyDye fluors followed by multiplexed two-dimensional separation. The intensity of protein spots and comparison of proteome maps was performed using DeCyder software. Identification of differentially regulated proteins was determined using nanoLC-ESI-MS/MS analysis. We found a total of 27 protein spots, 21 of which were unique proteins, which were differentially expressed in retinas with disorganized outer segments. We predict that in the absence of the RPE, oxidative stress initiates an unfolded protein response. Subsequently, downregulation of several candidate Müller glial cell proteins may explain the inability of photoreceptors to properly fold their outer segment membranes. In this study we have used identification and bioinformatics assessment of proteins that are differentially expressed in retinas with disorganized outer segments as a first step in determining probable key molecules involved in regulating photoreceptor outer segment assembly.

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Comparison between the Gene Expression Profile of Human Müller Cells and Two Spontaneous Müller Cell Lines

Abstract: The SAGE libraries reported in this article provide the gene expression profile of NHMCs and HMLCs. It thus represents a valuable source of information regarding the function of Müller cells as well as their role in the development of diabetic retinopathy.

Cited by 11 publications

References 95 publications

Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy

Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy

Comparison of Gene Expression Profile of Epiretinal Membranes Obtained from Eyes with Proliferative Vitreoretinopathy to That of Secondary Epiretinal Membranes

Proteomic analysis of the retina: Removal of RPE alters outer segment assembly and retinal protein expression

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