Comparison between two strategies for umbilical cord blood collection

Abstract

Summary:The use of cord blood (CB) for transplantation has increased greatly in recent years. The collection strategy is the first step in collecting good-quality CB units. There are two main techniques for collecting CB from the umbilical vein: in the delivery room while the placenta is still in the uterus by midwives and obstetricians or in an adjacent room after placental delivery by CB bank trained personnel. In this study, the benefits and disadvantages between the two different CB collection strategies were evaluated, in order to improve CB bank methodology. Valencia CB bank maintains the two different collection strategies. CB was obtained from 569 vaginal and 70 caesarean deliveries and obstetrical and clinical charts were reviewed. Before processing CB units, volume was calculated and samples were drawn for cell counts. After processing and before cryopreservation samples were drawn for cell counts, CD34+cell analysis, viability, clonogenic assays and microbiology were drawn directly from the bags. We compared the efficiency of the two collection techniques. Obstetric data and umbilical CB were obtained from 569 vaginal (264 collected in utero and 305 collected ex utero) and 70 caesarean deliveries. The proportion of excluded CB units before processing was 33% for vaginal ex utero, 25% for vaginal in utero and 46% for caesarean deliveries. Differences were statistically significant. For vaginal deliveries a larger volume and a higher number of nucleated cells, percentage of CD34+ cells and colony-forming units (CFUs) were harvested in the in utero collection group. There was no statistical difference between CB collected after placental expulsion from vaginal and caesarean deliveries. Comparison between all vaginal and caesarean deliveries did not show any difference. We conclude that the mode of collection influences the haematopoietic content of CB donations. Collection before placental delivery is the best approach to CB collection and allows optimisation of CB bank methodology. Caesarean deliveries seem to contain similar progenitor content to vaginal deliveries.

Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units

“…At present, there is no international consensus on the procedure of UCB collection in maternity wards [21]. Results from a recent study demonstrated that the median concentrations of nucleated cells and total colony-forming units were significantly lower in UCB collected after placental delivery when compared to their counterparts collected before placental delivery [22]. Our results are in agreement with those presented by Surbek et al [23] and show also higher numbers of total nucleated cells and colony-forming units for in utero cord blood collections.…”

Differential HIV-1 replication in neonatal and adult blood mononuclear cells is influenced at the level of HIV-1 gene expression

“…In general cord blood, samples are between 60 and 120 ml, yielding 300 to 600 million mononuclear cells. Cord blood yields more mononuclear cells than adult blood (31,32), so to obtain a comparable number of PBMCs, we collected 150-200 ml of adult blood. This study was approved by the University of Arizona Human Subjects Committee, and written informed consent was obtained from all donors.…”

Association of cord blood platelet count and volume with hemoglobin in healthy term infants

“…[1][2][3][4] A number of CB banks collect and store placental blood from healthy infants after uncomplicated pregnancy and delivery. 5,6 Data from CB banks have offered an acceptable alternative for studying blood cell physiology, such as CD34 þ cells, [7][8][9] because it can be demanding to ethically justify blood sampling from healthy newborn infants.…”