Comparison between Wound Healing in Induced Diabetic and Nondiabetic Rats after Low-Level Laser Therapy

Abstract

Objective: The aim of this work was to compare the effect of low-level laser therapy (LLLT) on the wound healing process in nondiabetic and diabetic rats. Background Data: Among the clinical symptoms caused by diabetes mellitus, a delay in wound healing is a potential risk for patients. It is suggested that LLLT can improve wound healing. Methods: The tissue used for this study was extracted from animals suffering from diabetes, which was induced by Streptozotocin ® , and from nondiabetic rats. Animals were assembled into two groups of 25 rats each (treated and control) and further subdivided into two groups: diabetic (n = 15) and nondiabetic (n = 10). A full-thickness skin wound was made on the dorsum area, with a round 8-mm holepunch. The treated group was irradiated by a HeNe laser at 632.8 nm, with the following parameters: 15 mW, exposition time of 17 sec, 0.025 cm 2 irradiated area, and energy density of 10 J/cm 2 . Square full-thickness skin samples (18 mm each side, including both injured and noninjured tissues) were obtained at 4, 7, and 15 days after surgery and analyzed by qualitative and quantitative histological methods. Results: Quantitative histopathological analysis confirmed the results of the qualitative analysis through histological microscope slides. When comparing tissue components (inflammatory cells, vessels and fibroblast/area), we found that treated animals had a less intense inflammatory process than controls. Conclusion: Results obtained by both qualitative and quantitative analyses suggested that irradiation of rats with HeNe (632.8 nm), at the tested dose, promoted efficient wound healing in both nondiabetic and diabetic rats as, compared to the control group. 474

Gingival Inflammation as a Sign of Diabetic Systemic Chronic Complications

“…These findings suggest that changes in the periodontal microvasculature are related to the level of metabolic control. The results of this investigation were similar to literature data 25 . The ''poor metabolic control'' shown through the high values of HbA1c clearly increases the risk of activation of gingival inflammation and higher values of Gi index are present as the value of HbA1c was rising (p<0,001).…”

Low-Level Laser Therapy with 810 nm Wavelength Improves Skin Wound Healing in Rats with Streptozotocin-Induced Diabetes

“…24 In this context, irradiation with HeNe laser at 10 J/ cm 2 in both nondiabetic and diabetic rats, significantly promoted wound healing when compared with their controls. 25 However, in diabetic patients, to stimulate angiogenesis, target tissues for LLLT are located deeper under the skin. From this perspective, the use of an infrared laser is justified, because of its greater depth of penetration.…”

Analysis of the Systemic Effect of Red and Infrared Laser Therapy on Wound Repair

“…Delays in wound healing usually result from complications such as bad nutrition, diabetes, malignancy, ischemia due to vascular disease, and advanced age. 15 Collagen output in wounds of patients with diabetes may be modulated using laser therapy. The process by which laser biostimulation facilitates collagen output in wound healing of patients with diabetes may involve several mechanisms.…”