Comparison of 2, 5, and 20 % O2 on the development of post-thaw human embryos

Yu, Yang; Xu, Yaowu; Ding, Chenhui; Khoudja, Rabea Youcef; Lin, Mingmei; Awonuga, Awoniyi O.; Dai, Jing; Puscheck, Elizabeth E.; Rappolee, Daniel A.; Zhou, Canquan

doi:10.1007/s10815-016-0693-5

Cited by 29 publications

(35 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yet, a recent randomized‐controlled trial reported improved blastocyst yields when embryos were sequentially exposed first to 5% oxygen from Days 1 to 3, and then to 2% oxygen from Days 3 to 5, compared to continuous exposure to 5% oxygen (Kaser, Bogale, Sarda, Farland, & Racowsky, ). These surprising results are difficult to resolve with previous data, unless the influence of cryopreservation of Day‐3 embryos (Yang et al, ) indirectly selected a population that is more resistant to oxidative stress. Therefore, more research needs to be conducted to understand the stage‐specific oxygen consumption of embryos and the physiologic oxygen concentration necessary for human embryo development—particularly to clarify how oxygen partial pressures during early in vitro development influences rates of implantation, pregnancy, and live birth.…”

Section: Embryo Development and The In Vivo Environmentmentioning

confidence: 65%

“…This reduced‐oxygen environment in the uterus may facilitate aerobic glycolysis and maintain pluripotency of embryonic cells—further supporting the hypothesis that ≤2% oxygen partial pressure may benefit blastocyst development. Intriguingly, post‐compaction, pre‐implantation embryo development in 2% oxygen also had adverse consequences for fetal development in the mouse (Feil et al, ) and for development from Day 3 to blastocysts in humans (Yang et al, ). One possible explanation for this unexpected result is that the original oxygen partial pressure measurements were inaccurate, as was determined for the concentration of other nutrients (see Table ).…”

Section: Embryo Development and The In Vivo Environmentmentioning

confidence: 99%

See 1 more Smart Citation

Metabolite availability as a window to view the early embryo microenvironment in vivo

2017

Molecular Reproduction Devel

View full text Add to dashboard Cite

A preimplantation embryo exists independent of blood supply, and relies on energy sources from its in vivo environment (e.g., oviduct and uterine fluid) to sustain its development. The embryos can survive in this aqueous environment because it contains amino acids, proteins, lactate, pyruvate, oxygen, glucose, antioxidants, ions, growth factors, hormones, and phospholipids-albeit the concentration of each component varies by species, stage of the estrous cycle, and anatomical location. The dynamic nature of this environment sustains early development from the one-cell zygote to blastocyst, and is reciprocally influenced by the embryo at each embryonic stage. Focusing on embryo metabolism allowed us to identify how the local environment was deliberately selected to meet the dynamic needs of the preimplantation embryo, and helped reveal approaches to improve the in vitro culture of human embryos for improved implantation rates and pregnancy outcome. | INTRODUCTIONThe basic energy needs-for example, glucose, pyruvate, and lactateof mammalian early embryos were determined for their in vitro culture nearly half a century ago (Biggers & Stern, 1973;Biggers, Whittingham, & Donahue, 1967), with the impact of endogenous fuels, including lipids and amino acids, identified soon thereafter (Chatot, Tasca, & Ziomek, 1990;Hillman & Flynn, 1980;Lane & Gardner, 1998;Quinn & Whittingham, 1982). Considerable progress has been made since these first studies, specifically defining a relatively complete system that accounts for the embryo's changing metabolism, which allowed for better reproducibility of in vitro culture as well as non-invasive assessment of embryo quality (Gardner & Leese, 1986;Gardner & Wale, 2013;Prasad, Tiwari, Koch, & Chaube, 2015;Houghton et al., 2002;Leese, Conaghan, Martin, & Hardy, 1993;Smith & da Rocha, 2012;Thompson, Brown, & Sutton-McDowall, 2016;Vajta, Rienzi, Cobo, & Yovich, 2010). Unfortunately, much of the data on embryo metabolism has come from in vitro studies (AbsalonMedina, Butler, & Gilbert, 2014;Gardner and Harvey, 2015;Leese, 2012Leese, , 2015Menezo, Lichtblau, & Elder, 2013

show abstract

Section: Embryo Development and The In Vivo Environmentmentioning

confidence: 65%

Section: Embryo Development and The In Vivo Environmentmentioning

confidence: 99%

Metabolite availability as a window to view the early embryo microenvironment in vivo

2017

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…In the results of that study, no differences were found in the blastulation rate among these groups. In studies of human embryos, there were no significant differences in the development rate to the blastocyst stage or in the number of high-quality blastocysts when embryos were cryopreserved on day 3 and cultured to the blastocyst stage at 2%, 5%, or 20% oxygen [29]. This study applied dynamic oxygen conditions, decreasing from 5% to 2%; these concentrations were determined through careful review of many previous studies and a brief preliminary experiment (data not shown).…”

Section: Discussionmentioning

confidence: 98%

Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system

Lee¹,

Seo²,

Lee

et al. 2019

Clin Exp Reprod Med

View full text Add to dashboard Cite

Objective:We aimed to evaluate the effects of different oxygen conditions (20% [high O2], 5% [low O2] and 5% decreased to 2% [dynamic O2]) on mouse pre-and peri-implantation development using a novel double-channel gas supply (DCGS) incubator (CNC Biotech Inc.) to alter the oxygen concentration during in vitro culture. Methods: The high-O2 and low-O2 groups were cultured from the one-cell to the blastocyst stage under 20% and 5% oxygen concentrations, respectively. In the dynamic-O2 group, mouse embryos were cultured from the one-cell to the morula stage under 5% O2 for 3 days, followed by culture under 2% O2 to the blastocyst stage. To evaluate peri-implantation development, the blastocysts from the three groups were individually transferred to a fibronectin-coated dish and cultured to the outgrowth stage in droplets. Results: The blastocyst formation rate was significantly higher in the low-O2 and dynamic-O2 groups than in the high-O2 group. The total cell number was significantly higher in the dynamic-O2 group than in the low-O2 and high-O2 groups. Additionally, the apoptotic index was significantly lower in the low-O2 and dynamic-O2 groups than in the high-O2 group. The trophoblast outgrowth rate and spread area were significantly higher in the low-O2 and dynamic-O2 groups than in the high-O2 group. Conclusion: Our results showed that a dynamic oxygen concentration (decreasing from 5% to 2%) had beneficial effects on mouse pre-and peri-implantation development. Optimized, dynamic changing of oxygen concentrations using the novel DCGS incubator could improve the developmental competence of in vitro cultured embryos in a human in vitro fertilization and embryo transfer program. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

show abstract

“…Yang et al studied 120 donated embryos that had been cultured at 20% oxygen concentration from fertilization until day 3 of development [35]. All embryos in this study had been cryopreserved on day 3.…”

Section: Ultra-low Oxygen Tension In Extended Culture: What Is the Cumentioning

confidence: 99%

Oxygen tension in embryo culture: does a shift to 2% O2 in extended culture represent the most physiologic system?

Morin

2017

J Assist Reprod Genet

View full text Add to dashboard Cite

There has been much debate regarding the optimal oxygen tension in clinical embryo culture. The majority of the literature to date has compared 5% oxygen to atmospheric levels (20-21%). While the majority of modern IVF labs have accepted the superiority of 5% oxygen tension, a new debate has emerged regarding whether a further reduction after day 3 of development represents the most physiologic system. This new avenue of research is based on the premise that oxygen tension is in fact lower in the uterus than in the oviduct and that the embryo crosses the uterotubal junction sometime on day 3. While data are currently limited, recent experience with ultra-low oxygen (2%) after day 3 of development suggests that the optimal oxygen tension in embryo culture may depend on the stage of development. This review article will consider the current state of the literature and discuss ongoing efforts at studying ultra-low oxygen tension in extended culture.

show abstract

Comparison of 2, 5, and 20 % O2 on the development of post-thaw human embryos

Abstract: The result suggests that development of cryopreserved human embryos from day 3 to blastocyst stage benefits from culture at 5 % O2.

Cited by 29 publications

References 56 publications

Metabolite availability as a window to view the early embryo microenvironment in vivo

Metabolite availability as a window to view the early embryo microenvironment in vivo

Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system

Oxygen tension in embryo culture: does a shift to 2% O2 in extended culture represent the most physiologic system?

Contact Info

Product

Resources

About