Comparison of a commercial modified direct agglutination test and a commercial enzyme-linked immunosorbent assay for screening for antibodies against Toxoplasma gondii in naturally exposed domestic cats

Comparison of Different Commercial Serology Kits for the Determination of Seropositive Toxoplasmosis in Cattle in Indonesia

“…In the present study, WB and MAT (Table 3 and Table 4) had an intermediate agreement with the Toxotest kit (Gwet's AC 1 = 0.458 and 0.557), similar to the report by Bărburaș et al (2019). The closeness of Toxotest kit as an ELISA method with MAT and WB demonstrates a consistent pattern with other reports such as Dubey et al (2005), Sroka et al (2008), Zhu et al (2012), Gu et al (2015), and Galat et al (2019). Therefore, the Toxotest kit can be considered to be applied together with or complementary to the WB and MAT.…”

Study of Cat Serum for Toxoplasmosis in Poltava

“…The test results are described in table (1,2,3). Antibodies to toxoplasmosis were detected in 14 females and the result was negative in 17 females.…”

Seroprevalence of Toxoplasma gondii in wild boars (Sus scrofa) hunted in Ukraine

“…The same locally available ELISA has been previously applied for samples from domestic cats ( Galat et al, 2019a ) and for samples from horses ( Rissanen et al, 2019 ). For domestic cats, the agreement with a commercial modified direct agglutination test (DAT) was 95.0% and Kappa for dichotomous screening outcome (seronegative or seropositive) was 0.8971, indicating almost perfect agreement ( Galat et al, 2019a ); whereas for horses, Kappa for dichotomous screening outcome was 0.3838, indicating fair agreement ( Rissanen et al, 2019 ). The results of this study indicate that for wild boar samples, the two ELISAs had moderate agreement with the IFAT.…”