Comparison of a competitive enzyme immunoassay kit and the infant mouse assay for detecting Escherichia coli heat-stable enterotoxin

Stavric, S.; Buchanan, Bob B.; Speirs, Joan I.

doi:10.1111/j.1472-765x.1992.tb00644.x

Cited by 5 publications

(3 citation statements)

References 9 publications

(12 reference statements)

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“…E. coli strains isolated from ice-cream samples in group B (unpacked-shop) and group C (unpacked -street vendors) in a percent of (50%), (55.5%) were enterotoxin producing and caused accumulation of fluids in the intestinal tract of the injected infant mice respectively. While Salmonella isolated in this study were unable to produce enterotoxin (Stavric et al, 1992 andWallis et al, 1986).…”

Section: Discussionmentioning

confidence: 59%

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Prevalence of Enteric Bacteria Producing Toxins in Ice-Cream and Kareish Cheese in Port-Said City Markets

2013

Assiut Veterinary Medical Journal

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The present study estimate the enteric bacteria in ice-cream and Kareish cheese. The study included 120 samples of ice-cream and Kareish cheese (60 of each), collected randomly from supermarkets, shops and street vendors in different districts of Port-Said city. All samples were examined for presence of Enterobacteriaceae spp. (on Violet Red Bile Glucose agar medium). Enterobacteriaceae strains were isolated in a percentage of 60% and 80% from group B (unpacked-shop) and group C (unpacked-street vendors) of ice-cream samples respectively, while the Enterobacteriaceae strains were isolated in a percentage of 40% and 93.3% from group B (unpacked-shop) and group C (unpacked-street vendors) of Kareish cheese samples respectively. The percentage of isolation of E. coli was 13.3% from group C (unpacked-street vendors) of ice-cream samples , while it was isolated in 6.6% from group B (unpacked-shop) and 60% from group C (unpacked-street vendors) of Kareish cheese samples. Salmonella spp. have been isolated in a high percentage (13.3%) from group C (unpacked-street vendors) of Kareish cheese samples and more than that of ice-cream (6.6%). The confirmation of the colonies was done biochemically and were identified up to species level. E.coli isolates from Kareish cheese in group C (unpacked-street vendors) were belonged to Poly(1) O:01, O55:K59 (B5), O124:K72 (B17) and in group B (unpacked-shop) were untyped, while E.coli isolates from ice-cream in group C (unpacked-street vendors) were untyped. Salmonella isolates from ice-cream and Kareish cheese were S. Typhimurium and S. paratyphi B. Studying the E.coli and Salmonella isolates for ability to produce toxin by using infant mouse assay was carried out. The isolated serotypes constitute public health hazards to consumers.

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Section: Discussionmentioning

confidence: 59%

“…The ability to produce enterotoxin was assayed by the infant mouse test according to the technique described by (Pai and Mors, 1978) and (Stavric et al, 1992).…”

Section: -Detection Of Toxin Produced By Isolates: Enterotoxin Assaymentioning

confidence: 99%

Prevalence of Enteric Bacteria Producing Toxins in Ice-Cream and Kareish Cheese in Port-Said City Markets

2013

Assiut Veterinary Medical Journal

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“…Scotland et al (1989) used the ST-EIA kit and infant mouse test to show that 100 E. coli strains with the ST A1 and ST A2 genes produced heatstable enterotoxin. The ELISA was easy to perform and gave results within 24 h. Stavric et al (1992) also compared the ST-EIA and infant mouse assay and found that 31 of 46 strains of E. coli were positive by both assays, while 15 strains were negative. They reported that the sensitivity of the ST-EIA kit was up to 64-fold lower than the infant mouse assay.…”

Section: Enteropathogenic Escherichia Colimentioning

confidence: 99%

A review of analytical methods for the detection of bacterial toxins

Pimbley

Patel

1998

Journal of Applied Microbiology

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Diarrhoea caused byEscherichia coli

Hart

Batt

Saunders

1993

Annals of Tropical Paediatrics

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Since the mid-1940s, Escherichia coli has been recognized as a cause of diarrhoea. Subsequently it has been shown that at least five different pathogenic mechanisms are used to cause disease. Enterotoxigenic E. coli (ETEC) and enteropathogenic E. coli (EPEC) produce a noninflammatory diarrhoea, whereas enteroinvasive E. coli (EIEC), enterohaemorrhagic E. coli (EHEC) and enteroaggregative E. coli (EAggEC) produce an inflammatory diarrhoea. ETEC are a major cause of diarrhoea in infants (up to three episodes per year) and travellers. They produce diarrhoea by attaching to the small intestinal mucosa and elaborate one or both of heat labile and heat stable toxins. EPEC attach firmly to the intestinal mucosa leading to dissolution of the brush border by inducing vesiculation of the microvilli. This process is known as attaching-effacement, and in the jejunum and ileum results in a loss of brush border disaccharidase enzymes and a large area of absorptive surface. EPEC are a major cause of summer diarrhoea in infants and neonatal diarrhoea. EIEC attach to colonic enterocytes, penetrate by an endocytotic mechanism and replicate therein. This results in necrosis and stripping of large areas of colonic mucosa and a dysentery similar to but usually less severe than Shigella dysentery. EHEC produce attaching-effacement to the terminal ileal and colonic mucosa and release the toxins, verocytotoxin (VT) 1 or 2. These kill colonic enterocytes and produce haemorrhagic colitis. In addition, VT can damage vascular endothelial cells, leading to haemolytic uraemic syndrome. The role of EHEC in diarrhoea in children in the tropics is not known. The most recently described group are the EAggEC. They damage and blunt colonic villi by haemorrhagic necrosis, although the precise pathogenic mechanisms are unclear. EAggEC are a major cause of chronic diarrhoea in children. Although certain O-serotypes are associated with the different enteropathic E. coli, serotyping is not sufficiently specific or sensitive for use as a diagnostic tool. Specific diagnosis is expensive and time consuming and depends upon demonstration of the pathogenicity trait, and the pathogenicity gene(s) or their gene product(s). At present, to undertake such testing is not recommended for routine diagnosis but is most useful when surveys of the aetiology of acute and chronic diarrhoea are undertaken.

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Comparison of a competitive enzyme immunoassay kit and the infant mouse assay for detecting Escherichia coli heat-stable enterotoxin

Cited by 5 publications

References 9 publications

Prevalence of Enteric Bacteria Producing Toxins in Ice-Cream and Kareish Cheese in Port-Said City Markets

Prevalence of Enteric Bacteria Producing Toxins in Ice-Cream and Kareish Cheese in Port-Said City Markets

A review of analytical methods for the detection of bacterial toxins

Diarrhoea caused byEscherichia coli

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