Comparison of a Direct ELISA and an HPLC Method for Glyphosate Determinations in Water

Abstract

A competitive direct enzyme-linked immunosorbent assay (ELISA) and high-pressure liquid chromatographic (HPLC) methods were compared in terms of accuracy and precision for the detection and quantification of glyphosate-spiked Nanopure, tap, and river waters. The ELISA had a detection limit of 0.6 ng mL(-)(1) and a linear working range of 1-25 ng mL(-)(1), whereas the HPLC method had a detection limit of 50 ng mL(-)(1) and a linear working range of 100-10000 ng mL(-)(l). No statistically significant differences (95% confidence interval) were found between the ELISA and HPLC analysis of the three water matrixes. The coefficients of variation obtained with the ELISA in tap water were between 10 and 19%, whereas the coefficients of variation for the HPLC analysis were between 7 and 15%. The use of ELISA for the analysis of glyphosate in water is a cost-effective and reliable method capable of meeting water quality guidelines established for Europe and North America.

Determination of Glyphosate in Water Samples by Multi-pumping Flow System Coupled to a Liquid Waveguide Capillary Cell

“…The limit of detection and quantification were 0.17 and 0.53 μg mL -1 , respectively. The LOD is consistent with the maximum contaminant level for drinking water in the United States of 0.7 μg mL -1 , 40 and also the concentration of 0.28 μg mL -1 recommended by Health and Welfare Canada in the Canadian Water Quality Guidelines. 40 …”

Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment

“…This lack of data may be linked to the high polarity of the molecule, close in structure to many naturally occurring amino acids, that makes its detection difficult in water samples (Skark et al, 1998;Rubio et al, 2003). This difficulty is probably worsened by the low concentrations occurring in coastal waters.…”

Glyphosate pathways to modern diseases VI: Prions, amyloidoses and autoimmune neurological diseases

“…It has been shown that both HPLC and ELISA are comparable in terms of accuracy and precision for detection and quantification of glyphosate in water-based analysis and including Nanopure, tap and river waters. Water-based solvents for glyphosate demonstrate a detection limit of 0.6 ng/mL and a linear functional range of 1-25 ng/mL [200]. However, HPLC was not able to achieve detection below 5 ppb; 1 hence, in cases including water-based vaccines, analysis using numerous sample runs was made including using two independent labs to test the same samples.…”