Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays

Peng, Dapeng; Hu, Sishun; Yan, Hua; Xiao, Yufei; Li, Zili; Wang, Xiliang; Bi, Dingren

doi:10.1016/j.vetimm.2007.01.022

Cited by 73 publications

(47 citation statements)

References 17 publications

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“…Highly pathogenic avian influenza viruses can be easily transmitted between poultry production facilities, leading to severe disease outbreaks or even pandemics. It is difficult to control the spread, so all chickens in facilities are usually slaughtered (Peng et al, 2007). All causes enormous economic losses in the poultry industry and seriously threatens human health (Dhumpa et al, 2011;Guan et al, 2002;Shortridge et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…All causes enormous economic losses in the poultry industry and seriously threatens human health (Dhumpa et al, 2011;Guan et al, 2002;Shortridge et al, 1998). To prevent and control AI in poultry, vaccination has been employed as a key strategy in many countries since the 1990s (Nilsson et al, 2010;Peng et al, 2007). According to European regulation (Council Directive 2005/94/EC) programmes of industrial birds vaccination against AI, the DIVA (Differentiating Infected from Vaccinated Animals) strategy should be applied in order to reduce the risk of a "silent spread" of the virus due to incomplete protection at a flock level.…”

Section: Introductionmentioning

confidence: 99%

“…The conventional methods most frequently used for detection of antibodies against influenza A virus are enzyme-linked immunosorbent assays (ELISA) (Chen et al, 2011;Ciacci-Zanella et al, 2010;Hoque et al, 2012;Lebarbenchon et al, 2012;Moreno et al, 2013), hemagglutination inhibition (HI) (Allwinn et al, 2010;Schultsz et al, 2009;Peng et al, 2007) and Western blot assay (WB) (Uyeki et al, 2012). Nevertheless, they are often laborious and time-consuming or need expensive instruments.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum

Jarocka

Sawicka

Góra-Sochacka

et al. 2014

Biosensors and Bioelectronics

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This paper describes the development of an immunosensor for detection of anti-hemagglutinin antibodies. Its preparation consists of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with EDC/NHS coupling reaction, (iii) covering with anti-His IgG monoclonal antibody, (iv) immobilization of the recombinant His-tagged hemagglutinin (His6-H5 HA), (v) filling free space with BSA. The interactions between two variants of recombinant HA (short and long) from highly pathogenic avian influenza virus H5N1 and the anti-H5 HA monoclonal antibody (Mab 6-9-1) have been explored with electrochemical impedance spectroscopy (EIS). The impedimetric immunosensor displayed a very good detection limit (LOD) of 2.1 pg/mL, the quantification limit (LOQ) of 6.3 pg/mL and a dynamic range from 4 pg/mL to 20 pg/mL. In addition, this analytical device was applied for detection of antibodies against His6-H5 HA in serum of vaccinated hen using serial 10-fold dilutions of serum. The immunosensor proposed was able to detect antibody in hen serum diluted up to 7 x 10 7 -fold.The sensitivity of immunosensor was about four orders of magnitude much better than ELISA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum

Jarocka

Sawicka

Góra-Sochacka

et al. 2014

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…The MBLF technology was also combined with multiplex-nested Polymerase Chain Reaction (PCR) to detect various antibodies [6], [7], antigens [8], [9], and allergens [10], [11]. Together these studies have prompted considerable interest in MBLF strips for its potential applications in detection of deoxyribonucleic acids of various pathogens.…”

Section: Introductionmentioning

confidence: 99%

Detection of Deoxyribonucleic Acids on A Membrane Strip

Wu¹,

Hsu²,

Cheng³

et al. 2013

IJBBB

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Abstract-A lateral-flow membrane strip was developed to detect the signal of deoxyribonucleic acids. The signal amplification was demonstrated by the detection of the model sample, biotinlyated Avian-Influenza (AI) genetic sequence, with a sandwich configuration of the target-RCA template complex captured between a RCA (Rolling Circle Amplification) template and streptavidin protein immobilized on a nitrocellulose membrane strip.

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“…Recently, it has been efficiently applied to the detection of bovine virus diarrhea and white spot syndrome viruses (15,27). We have successfully developed immunochromatographic strips for detecting antibodies against AIV (22) and detecting sulfadiazine residue in eggs and chickens (28,29). To extend these studies, we have recently developed an immunochromatographic strip which can specifically, sensitively, and rapidly detect H9AIV.…”

mentioning

confidence: 99%

Development of an Immunochromatographic Strip for Rapid Detection of H9 Subtype Avian Influenza Viruses

Peng

Wang

Zhang

et al. 2008

Clin Vaccine Immunol

Self Cite

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An immunochromatographic strip was developed for the detection of the H9 subtype of avian influenza viruses (H9AIVs) in poultry, using two monoclonal antibodies (MAb), 4C4 for H9AIV hemagglutinin (HA) and 4D4 for nucleoprotein. The 4C4 MAb was labeled with colloidal gold as the detection reagent, and the 4D4 MAb was blotted on the test line while a goat anti-mouse antibody was used on the control line of the nitrocellulose membrane. In comparison with the HA and HA inhibition (HI) tests, the strip was specific for the detection of H9AIV, with a sensitivity at 0.25 HA units within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity and specificity. Evaluation of the strip with experimental tracheal and cloacal swab samples collected from H9N2-infected chickens revealed that the strip detected the H9N2 viruses on day 3 postinoculation, earlier than the appearance of clinical symptoms. Application of the strip for the analysis of 157 tracheal or cloacal samples from potentially infected chickens on five poultry farms showed that four farms had chickens that were infected with H9AIV. Further characterization of 10 positive and 30 negative randomly selected samples showed that no single sample was false positive or negative, as determined by the standard virus isolation and HI assays. Therefore, the immunochromatographic strip for the detection of H9AIVs has high specificity, sensitivity, and stability. This finding, together with the advantages of rapid detection and easy operation and without the requirement for special skills and equipment, makes the strip suitable for onsite detection and the differentiation of H9AIVs from other viruses in poultry.

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Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays

Cited by 73 publications

References 17 publications

Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum

Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum

Detection of Deoxyribonucleic Acids on A Membrane Strip

Development of an Immunochromatographic Strip for Rapid Detection of H9 Subtype Avian Influenza Viruses

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