Comparison of a new microscopic system for the measurement of residual leucocytes in apheresis platelets with flow cytometry and manual counting

Abstract

In comparison with manual counting and flow cytometry, the ADAM-rWBC device performed equally. The method is suitable for routine screening of leucocyte contamination of apheresis platelets.

Evaluation of ADAM‐rWBC for counting residual leucocytes in leucocyte‐reduced whole blood and apheresis platelet concentrates

“…Similar results were obtained in our experiments with PCs, which were collected by three different apheresis systems. Our results generally agree with the previous report by Strobel et al (2014). They reported that ADAM-rWBC had good linearity in the range of 0·032-128 and 0·1-8 cells μL −1 in the experiments with PCs, which were collected by Trima Accel.…”

“…Therefore, for the dilution studies, we prepared samples with a range of leucocyte concentrations including 3 cells μL −1 and 5 cells μL −1 . We set an upper limitation of the leucocyte concentration at 30 cells μL −1 , which is lower than around the 100 cells μL −1 used in previous studies (Kline et al, 2012;Strobel et al, 2014). Samples for dilution studies were prepared as follows: unfiltered WBs (n = 3) and apheresis PCs (n = 3) were divided into two parts.…”

Methods for counting residual leukocytes in leukocyte‐depleted plasma—a comparison between a routine hematology instrument, the Nageotte chamber, flow cytometry, and a fluorescent microscopy analyzer

“…The ADAM‐rWBC has previously been found suitable for measuring residual WBCs in platelet concentrates and in whole blood . In the study by Strobel and coworkers, the results from ADAM‐rWBC and flow cytometry analyses were comparable, whereas the Nageotte chamber produced results with a negative bias and had lower accuracy.…”

“…Polymerase chain reaction methods have also been suggested but were regarded expensive and even more laborious than the Nageotte chamber method . Recently, a dedicated fluorescent microscopy analyzer was introduced with promising results, but the instrument had previously been tested only on platelet concentrates and in leukocyte‐depleted whole blood …”

Neutrophil extracellular traps in stored canine red blood cell units

“…This assay has been previously validated in canine RBC units 18 . Leukocyte counts from NLR units were reported from the automated hematology analyzer whereas leukocyte counts from the LR units were reported from the flow cytometric method, as these counts were below the limit of detection of the automated hematology analyzer 19 . Successful LR was defined per human blood banking standards as less than 5 × 10 6 white cells per unit 20…”