Comparison of a specific HPLC determination of toxic aconite alkaloids in processed Radix aconiti with a titration method of total alkaloids

Csupor, Dezső; Borcsa, Botond; Heydel, B.; Hohmann, Judit; Zupkó, István; Ma, Yue; Widowitz, U; Bauer, Rudolf

doi:10.3109/13880209.2011.595011

Cited by 16 publications

(22 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initially, the acetyl group is hydrolyzed and in the second step, the benzoyl group is hydrolyzed (l " Fig. 2) [17]. This process results in an increase in the concentrations of monoester-diterpenoid alkaloids (benzoylaconine, benzoylmesaconine, and benzoylhypaconine) in the processed Fuzi [18].…”

Section: Traditional Chinese Medicinementioning

confidence: 99%

“…In the USA and Canada, there are no specific regulations on Aconitum preparations, and reported toxic cases were mainly due to misidentification and misuse of the herb [122]. Different analytical techniques were developed to analyze and quantify the concentrations of aconitine alkaloids, including HPLC, LC-MS, LC-MS/MS, and electrophoresis, with impressive accuracy and speed [3,17,18].…”

mentioning

confidence: 99%

“…ite preparation using trivial analytical equipment available in almost all analytical laboratories [13][14][15][16][17][18]. Also, the biological effects of different Aconitum sp.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Clinical Aspects of Aconitum Preparations

Tai

El-Shazly

et al. 2015

Planta Med

Self Cite

View full text Add to dashboard Cite

Aconitum, also known as monkshood, wolfʼs bane, or devilʼs helmet, has been widely used in folk medicine in China, India, and certain parts of Europe [1-4]. The genus Aconitum (Ranunculaceae) comprises 300 species distributed all over the world [5]. The most common species are Aconitum carmichaelii Deb. and Aconitum kusnezoffii Rchb. in China, Aconitum japonicum Thunb. in Japan, Aconitum napellus L. in Europe, Aconitum ferox Wall. ex Ser. in India, and Aconitum noveboracense A. Gray ex Coville in the United States [5]. Several classes of secondary metabolites, especially alkaloids, have been isolated from different Aconitum sp. [6]. The type of the isolated major alkaloids may vary depending on the species such as aconitine, hypaconitine, and mesaconitine from A. carmichaelii, aconitine from A. napellus, hypaconitine from A. septentrionale Koelle, mesaconitine from A. kusnezoffii, bikhaconitine from A. ferox, talatisamine from A. kongboense Lauener, atisine from A. anthora L., and A. heterophyllum Wall. ex Royle, and lycaconitine from A. vulparia Rchb. [6]. Several isoquinoline alkaloids and phenethylamine derivatives have also been isolated, such as higenamine from A. japonicum, magnoflorine from A. vulparia and A. napellus, coryneine from A. carmichaelii, and N-methyl adrenaline from A. nasutum Fisch. ex Rchb. [6]. Lipo-alkaloids including lipoaconitines, lipomesaconitines, lipodeoxyaconitines, and lipohypaconitines were also isolated [7, 8]. The efficacy of Aconitum sp. in resolving critical clinical conditions has been proven by doctors practicing traditional Chinese medicine (TCM) and Ayurvedic medicine for centuries. However, the long history of Aconitum sp. misuse in homicide cases has shaken the faith in the potential safe application of this herb in therapy [9, 10]. The recent developments in analytical techniques which can identify and determine the concentrations of toxic compounds in herbal products with impressive accuracy and reliability have rekindled the interest in Aconitum preparations [1, 5, 6, 11, 12].

show abstract

Section: Traditional Chinese Medicinementioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Clinical Aspects of Aconitum Preparations

Tai

El-Shazly

et al. 2015

Planta Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Aconitine, mesaconitine, and hypaconitine were purchased from PhytoLab GmbH. 82 For HPLC, an improved version of a recently reported method has been applied. 82 Peaks of mesaconitine, aconitine, and hypaconitine were identified by comparison of the HPLC-DAD chromatograms of the extracts of aconite roots with those of the reference solutions.…”

Section: Determination Of Toxic Alkaloid Contents Of Processed Radix mentioning

confidence: 99%

“…82 For HPLC, an improved version of a recently reported method has been applied. 82 Peaks of mesaconitine, aconitine, and hypaconitine were identified by comparison of the HPLC-DAD chromatograms of the extracts of aconite roots with those of the reference solutions. Alkaloid content was calculated by comparison of the sum of the areas under curves (AUC) of mesaconitine, aconitine, and hypaconitine on the basis of the calibration curve established for aconitine.…”

Section: Determination Of Toxic Alkaloid Contents Of Processed Radix mentioning

confidence: 99%

Investigations of diterpene alkaloids isolated from Aconitum anthora L. and A. moldavicum L., and of aconitine-derived lipo-alkaloids

Lajos¹

View full text Add to dashboard Cite

Anti‐rheumatic potential vis‐à‐vis aconitine and hypaconitine content analysis in different Aconitum spp. from Sikkim Himalayas (India)

Kumar,

Misra,

Rawat

et al. 2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

Aconitum spp. are important medicinal plants mentioned in Ayurveda as Ativisa or Vatsanabha. The present study aims to evaluate anti‐rheumatic potential in seven Aconitum species and correlation with aconitine and hypaconitine content. Anti‐rheumatic potential was analyzed through in vitro xanthine oxidase inhibition, anti‐inflammatory and ROS scavenging assays; and quantification of aconitine and hypaconitine with RP‐HPLC method validated as per ICH guidelines. The findings reveal that A. palmatum possessed the most promising response (IC50 = 12.68±0.15 µg/ml) followed by A. ferox (IC50= 12.912±1.87 µg/ml) for xanthin oxidase inhibition. We observed a wide variation in aconitine and hypaconitine content ranging from 0.018%‐1.37% and 0.0051%‐0.077% respectively on dry weight basis. Aconitine and hypaconitine showed moderate positive correlation (r= 0.68 and 0.59 respectively) with anti‐rheumatic potential. The study identifies potential alternative species of Aconitum that can help in sustainable availability of quality raw material.

show abstract

Comparison of a specific HPLC determination of toxic aconite alkaloids in processed Radix aconiti with a titration method of total alkaloids

Abstract: (2011) Comparison of a specific HPLC determination of toxic aconite alkaloids in processed Radix aconiti with a titration method of total alkaloids,

Cited by 16 publications

References 13 publications

Clinical Aspects of Aconitum Preparations

Clinical Aspects of Aconitum Preparations

Investigations of diterpene alkaloids isolated from Aconitum anthora L. and A. moldavicum L., and of aconitine-derived lipo-alkaloids

Anti‐rheumatic potential vis‐à‐vis aconitine and hypaconitine content analysis in different Aconitum spp. from Sikkim Himalayas (India)

Contact Info

Product

Resources

About