Comparison of accuracy, precision, and sensitivity in elemental assays of fish otoliths using the electron microprobe, proton-induced X-ray emission, and laser ablation inductively coupled plasma mass spectrometry

Campana, S.E.; Thorrold, Simon R.; Jones, C.M.; Günther, D.; Tubrett, M.; Longerich, H.; Jackson, S.; Halden, Norman M.; Kalish, J.M.; Piccoli, P.; Pontual, Hélène de; Troadec, H.; Panfili, J.; Secor, David H.; Severin, K.P.; Sie, S.H.; Thresher, Ronald E.; Teesdale, W.J.; Campbell, John L.

doi:10.1139/cjfas-54-9-2068

Cited by 65 publications

(59 citation statements)

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“…CaCO 3 accounts for 96.2% of otolith weight and normalization of element contents in otoliths to Ca can effectively reduce inter-sample variance [21] . So, the contents of ferromagnetic elements (Fe, Co, Ni) and other metal elements in the lagenal, saccular and utricular otolith samples of adult homing pigeons were normalized to the content of Ca in the corresponding samples.…”

Section: Discussionmentioning

confidence: 99%

信鸽瓶状囊耳石中磁性元素的电感耦合等离子体质谱分析

et al. 2009

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Objective The macula lagena in birds is located at the apical end of the cochlea and contains many tiny otoliths.The macula lagena is innervated and has neural projections to the brainstem, but its physiological function is still unclear. It remains disputable that it is because otoliths in the lagena are rich in elements Fe and Zn that birds can obtain geomagnetic information for homing. To clarify this issue, we carried out a study to determine whether or not otoliths in the lagena of homing pigeons are richer in magnetic elements than those in the saccule and the utricle. Methods The contents of ferromagnetic elements (Fe, Co, Ni) and other metal elements in lagenal otoliths of adult homing pigeons were precisely analyzed with inductively coupled plasma mass spectrometry (ICP-MS) of high sensitivity, and then they were compared with those in saccular and utricular otoliths (all the contents were normalized to Ca). Results In adult homing pigeons, the contents of ferromagnetic elements (Fe, Co, Ni) in lagenal otoliths were less than 0.7% (normalized to Ca element) and were the same order in magnitude as those in saccular and utricular otoliths. The content of Fe in lagenal otoliths was not significantly different from that in utricular otoliths and was even lower than that in saccular otoliths. The content of Co in lagenal otoliths was lower than that in saccular otoliths and higher than that in utricular otoliths. The content of Ni in lagenal otoliths was not significantly different from that in saccular otoliths and was higher than that in utricular otoliths. The contents of other metal elements Na, Mg, K, Al, Mn and Pb in lagenal otoliths were not significantly different from those in utricular and saccular otoliths. The contents of metal elements Zn, Ba and Cu in lagenal otoliths were lower than those in saccular otoliths. ConclusionThe contents of magnetic elements in lagenal otoliths of homing pigeons are not much higher than those in utricular and saccular otoliths, which does not support the hypothesis that birds depend on high contents of Fe and Zn in lagenal otoliths for sensation of geomagnetic information. Similarities in morphology, element ingredient and element content between lagenal otoliths and utricular otoliths suggest that the two types of otolithic organs may play similar roles in sensing gravitational and acceleration signals.

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Section: Discussionmentioning

confidence: 99%

信鸽瓶状囊耳石中磁性元素的电感耦合等离子体质谱分析

et al. 2009

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“…A lab standard, consisting of Micropogonias undulatus otoliths ground to micron-sized particles (Campana et al 1997), was also assayed periodically throughout the otolith analysis to assess measurement repeatability. All elemental data were subsequently converted to molar concentrations and expressed as ratios to Ca.…”

Section: Methodsmentioning

confidence: 99%

Accurate classification of juvenile weakfish Cynoscion regalis to estuarine nursery areas based on chemical signatures in otoliths

Thorrold¹,

Jones²,

Swart³

et al. 1998

Mar. Ecol. Prog. Ser.

192

160

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We investigated the ability of trace element and isotopic signatures in otoliths to record the nursery areas of juvenile (young-of-the-year) weakfish Cynoscion regalis from the east coast of the USA. Juvenile C. regalis were captured with otter trawls at multiple sites in Doboy Sound (Georgia), Panllico Sound (North Carolina), Chesapeake Bay (Virginia), Delaware Bay (Delaware) and Peconic Bay (New York), from July to September 1996. One sagittal otolith from each specimen was assayed for Mg/Ca, Mn/Ca, Sr/Ca and Ba/Ca ratios uslng inductively coupled plasma mass spectrometry (ICP-MS), while 6I3C and 6180 values from the other sagittal otohth in the pair were determined using lsotope ratio mass spectrometry (IR-MS). A multivanate analysis of variance determined that there were significant differences in trace element signatures among locations. Bootstrapped 95% confidence ellipses on canonical variates indicated that all 5 locations were significantly isolated in discriminant space. On the basis of these differences, linear &scrirninant function analysis (LDFA) and artificial neural network (ANN) models were used to classify inbvidual fish to their natal estuary with an overall error rate of 37 % for LDFA and 29.6% for ANN. Addtion of 6I3C and 6180 values to the LDFA and ANN models derived from the trace element data resulted in overall error around 10%. We d, therefore, be able to use chemical signatures from the juvenile portion of adult C, reyalis otoliths to accurately classify these fish to their natal estuary.

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“…There are a wide variety of sophisticated instruments available for probed assays of the otolith (Coutant 1990, Jackson et al 1993, Gauldie et al 1994, Jambers et al 1995, but the most frequently used include the energy-dispersive (ED-EM) and wavelength-dispersive (WD-EM) electron microprobes (Gunn et al 1992), proton-induced X-ray emission (PIXE) (Sie & Thresher 1992), and laser ablation ICPMS (LA-ICPMS) (Campana et al 1994). In a detailed experimental comparison among the above instruments, Campana et al (1997) noted that no one instrument type was sensitive to each element, nor was any one instrument preferred for use in all assays. In general, however, the minor elements such as Na and K could only be measured accurately with an electron microprobe, while the trace elements required PIXE or LA-ICPMS.…”

Section: Sampling and Analysesmentioning

confidence: 99%

Chemistry and composition of fish otoliths:pathways, mechanisms and applications

Campana¹

1999

Mar. Ecol. Prog. Ser.

1,810

1,932

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ABSTRACT:The fish otolith (earstone) has long been known as a timekeeper, but interest in its use as a metabolically inert environmental recorder has accelerated in recent years. In part due to technological advances, applications such as stock identification, determination of rnigralon pathways, reconstruction of temperature and salinity history, age validation, detection of anadromy, use as a natural tag and chemical mass marking have been developed, some of which are difficult or impossible to implement using alternative techniques. Microsamphg and the latest advances in beam-based probes allow many elemental assays to be coupled with daily or annual growth increments, thus providing a detailed chronologcal record of the environment. However, few workers have critically assessed the assumptions upon which the environmental reconstructions are based, or considered the possibility that elemental incorporation into the otolith may proceed differently than that into other calcified structures. This paper reviews current applications and their assumptions and suggests future directions. Particular attention is given to the premises that the elemental and isotopic composition of the otolith reflects that of the environment, and the effect of physiological filters on the resulting composition. The roles of temperature, elemental uptake into the fish and the process of otolith crystahzation are also assessed. Drawing upon recent advances i n geochemistry and paleoclirnate research as points of contrast, it appears that not all applications of otolith chemistry are firmly based, although others are destined to become powerful and perhaps routine tools for the mainstream fish biologst.

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Comparison of accuracy, precision, and sensitivity in elemental assays of fish otoliths using the electron microprobe, proton-induced X-ray emission, and laser ablation inductively coupled plasma mass spectrometry

Cited by 65 publications

References 0 publications

信鸽瓶状囊耳石中磁性元素的电感耦合等离子体质谱分析

信鸽瓶状囊耳石中磁性元素的电感耦合等离子体质谱分析

Accurate classification of juvenile weakfish Cynoscion regalis to estuarine nursery areas based on chemical signatures in otoliths

Chemistry and composition of fish otoliths:pathways, mechanisms and applications

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