Comparison of Actin- and Glass-Supported Phospholipid Bilayer Diffusion Coefficients

Sterling, Sarah M.; Dawes, Ryan P.; Allgeyer, Edward S.; Ashworth, Sharon; Neivandt, David J.

doi:10.1016/j.bpj.2015.02.033

Cited by 14 publications

(12 citation statements)

References 66 publications

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“…In a simplified view of the fusion process, a highly curved membrane can provide some of the driving force that reduces the energetic barrier for the fusion process, and it has therefore long been believed that membrane curvature is an important modulator of fusion (Bassereau et al, ; Churchward et al, ; Churchward et al, ; Grafmuller et al, ). The vesicle membrane is highly curved, in contrast to the generally more rigid and planar pre‐synaptic plasma membrane with its underlying actin lattice (Jarsch et al, ; Sterling et al, ). The pre‐synaptic plasma membrane is also characterized by ‘islands’ of high and low fluidity (i.e.…”

Section: Fusogenic Lipids and Membrane Topologymentioning

confidence: 99%

Phospholipases in neuronal function: A role in learning and memory?

Joensuu

Wallis

Saber

et al. 2020

Journal of Neurochemistry

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| LIPIDS AND THE B R AINUnderstanding how the over 80 billion neurons in the human brain communicate with each other to generate a self-aware mind remains one of the great challenges of modern neurobiology. Despite decades of ever more sophisticated study and progress, the molecular mechanisms underpinning the exquisitely regulated neuronal communication at the heart of cognition, learning and memory remain incompletely understood. The discovery that neurotransmission is quantal (Del Castillo and Katz, 1954) led to the development of the vesicle hypothesis in which neurotransmitters are released following fusion of synaptic vesicles with the pre-synaptic membrane in a process known as neuroexocytosis. Decades of subsequent research validated this fundamental finding, and demonstrated the involvement of sophisticated protein machinery (e.g. SNAREs, N-ethylmaleimide-sensitive factor attachment receptors) in all aspects of vesicle trafficking. Most recently, the analysis of brain lipids and lipid metabolites has started to shift this somewhat 'proteocentric' view of neurotransmission to a more holistic view involving tightly regulated protein-protein, protein-lipid (Wenk and De Camilli, 2004) and lipid-lipid interactions, all of which are essential for neuronal communication.Indeed, the healthy human brain is composed of approximately 60% lipid by dry weight, higher than in any other tissue. Seminal chromatography experiments (O'Brien and Sampson, 1965) AbstractDespite the human brain being made of nearly 60% fat, the vast majority of studies on the mechanisms of neuronal communication which underpin cognition, memory and learning, primarily focus on proteins and/or (epi)genetic mechanisms. Phospholipids are the main component of all cellular membranes and function as substrates for numerous phospholipid-modifying enzymes, including phospholipases, which release free fatty acids (FFAs) and other lipid metabolites that can alter the intrinsic properties of the membranes, recruit and activate critical proteins, and act as lipid signalling molecules. Here, we will review brain specific phospholipases, their roles in membrane remodelling, neuronal function, learning and memory, as well as their disease implications. In particular, we will highlight key roles of unsaturated FFAs, particularly arachidonic acid, in neurotransmitter release, neuroinflammation and memory.In light of recent findings, we will also discuss the emerging role of phospholipase A 1 and the creation of saturated FFAs in the brain. K E Y W O R D S exocytosis, free fatty acids, learning, memory, phospholipases, phospholipids, synaptic plasticity | 301 JOENSUU Et al.

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Section: Fusogenic Lipids and Membrane Topologymentioning

confidence: 99%

Phospholipases in neuronal function: A role in learning and memory?

Joensuu

Wallis

Saber

et al. 2020

Journal of Neurochemistry

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“…al. also fabricated actin-supported bilayers, in which the actin cushion attempted to better mimic the cortex; the results of this work emphasize that fluidity modulation is polymer specific [107]. Alternatively, cushioning the SLB with a thin cellulose cushion has been used to generate homogeneous bilayers with mobile integrins [104].…”

Section: Supported Lipid Bilayers Technologiesmentioning

confidence: 99%

Supported lipid bilayer platforms to probe cell mechanobiology

Glazier¹,

Salaita²

2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Mammalian and bacterial cells sense and exert mechanical forces through the process of mechanotransduction, which interconverts biochemical and physical signals. This is especially important in contact-dependent signaling, where ligand-receptor binding occurs at cell-cell or cell-ECM junctions. By virtue of occurring within these specialized junctions, receptors engaged in contact-dependent signaling undergo oligomerization and coupling with the cytoskeleton as part of their signaling mechanisms. While our ability to measure and map biochemical signaling within cell junctions has advanced over the past decades, physical cues remain difficult to map in space and time. Recently, supported lipid bilayer (SLB) technologies have emerged as a flexible platform to measure and manipulate membrane receptor mechanotransduction, allowing one to mimic cell-cell junctions. Changing the lipid composition and underlying substrate tunes bilayer fluidity, and lipid and ligand micro- and nano-patterning spatially control positioning and clustering of receptors. Patterning metal gridlines within SLBs introduces corrals that confine lipid mobility and introduce mechanical resistance. Here we review fundamental SLB mechanics and how SLBs can be engineered as tunable cell substrates for mechanotransduction studies. Finally, we highlight the impact of this work in understanding the biophysical mechanisms of cell adhesion.

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“…demonstrated that lipids deposited on actin coated glass slides had diffusion coefficients 50% lower than lipids on uncoated glass. [31] Furthermore, Scomparin et. al.…”

Section: Discussionmentioning

confidence: 99%

Anisotropic biodegradable lipid coated particles for spatially dynamic protein presentation

Meyer

Mathew²,

Ben-Akiva³

et al. 2018

Acta Biomaterialia

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The research reported here documents the ability of non-spherical polymeric particles to be coated with lipids to form anisotropic biomimetic particles. In addition, we demonstrate that these lipid-coated biodegradable polymeric particles can be conjugated to a wide variety of biological molecules in a "click-like" fashion. This is of interest due to the multiple types of cellular mimicry enabled by this biomaterial based technology. These features include mimicry of the highly anisotropic shape exhibited by cells, surface presentation of membrane bound protein mimetics, and lateral diffusivity of membrane bound substrates comparable to that of a plasma membrane. This platform is demonstrated to facilitate targeted cell binding while being resistant to non-specific cellular uptake. Such a platform could allow for investigations into how physical parameters of a particle and its surface affect the interface between biomaterials and cells, as well as provide biomimetic technology platforms for drug delivery and cellular engineering.

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Comparison of Actin- and Glass-Supported Phospholipid Bilayer Diffusion Coefficients

Cited by 14 publications

References 66 publications

Phospholipases in neuronal function: A role in learning and memory?

Phospholipases in neuronal function: A role in learning and memory?

Supported lipid bilayer platforms to probe cell mechanobiology

Anisotropic biodegradable lipid coated particles for spatially dynamic protein presentation

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