Anisotropic biodegradable lipid coated particles for spatially dynamic protein presentation

Abstract

The research reported here documents the ability of non-spherical polymeric particles to be coated with lipids to form anisotropic biomimetic particles. In addition, we demonstrate that these lipid-coated biodegradable polymeric particles can be conjugated to a wide variety of biological molecules in a "click-like" fashion. This is of interest due to the multiple types of cellular mimicry enabled by this biomaterial based technology. These features include mimicry of the highly anisotropic shape exhibited by cells, surface presentation of membrane bound protein mimetics, and lateral diffusivity of membrane bound substrates comparable to that of a plasma membrane. This platform is demonstrated to facilitate targeted cell binding while being resistant to non-specific cellular uptake. Such a platform could allow for investigations into how physical parameters of a particle and its surface affect the interface between biomaterials and cells, as well as provide biomimetic technology platforms for drug delivery and cellular engineering.

Shape Shifting of Cup Shaped Particles on Growing poly (2‐hydroxy ethyl methacrylate) Brushes by “Grafting From” Approach and Dissipative Particle Dynamics Simulation

“…Among all the particle properties, particle shape has been found to have profound influence on the physiological interactions such as opsonization, internalization, margination, circular half‐life etc . In the beginning, spherical particles were extensively used as drug carriers but other non‐spherical particles such as cups, discs, rods, ellipsoids, tubes, cubic particles, worm shaped and wires have recently emerged as new drug carriers for controlled and targeted drug delivery applications . Apart from shape and size, the influence of surface chemistry was also found to be significantly important in guiding cell‐material interactions, crucial for many biomedical applications including targeted cell/drug delivery…”

The Utilization of Poly(2‐ethyl‐2‐oxazoline)‐b‐Poly(ε‐caprolactone) Ellipsoidal Particles for Intracellular BIKDDA Delivery to Prostate Cancer

“…One of the most important advantages of ellipsoidal particles as a drug delivery nanocarrier is membrane structure that is similar to the cell membrane, which ensures to carry different chemical agents, provides easily applicable chemical modifications, higher loading capacity, and no toxicity. [ 3 ]…”

Fabrication of amine‐decorated nonspherical microparticles with calcium peroxide cargo for controlled release of oxygen

“…Carrier characteristics such as composition, geometry, size, surface chemistry, density, and so forth can modulate oxygen release profile. Recently, nonspherical carriers in forms of ellipsoidal (oblate and prolate) (Desai et al, ; Meyer et al, ), discoidal (Wang et al, ), cylindrical (Li, Suzuki, & Minami, ), rod shaped (Cao et al, ), tubes (Avani, Damoogh, Mottaghitalab, Karkhaneh, & Farokhi, ; Liu, Xu, Liao, Fang, & Guo, ; Pardo, Peng, & Leblanc, ), wires (Heidarshenas, Wei, Moghimi, Hussain, & Naghieh, ), and so forth have attracted increasing attention for delivery of biological cargoes. Carrier geometry strongly affects transportation of loaded and surrounding molecules into or out of carriers thus controlling release profile.…”