Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles

Cha, Kyungeun; Hong, Hyewon; Choi, Yeon-Gil; Lee, Min Joo; Park, Jong Hoon; Chae, Heekwon; Ryu, Gyu Ha; Myung, Heejoon

doi:10.1007/s10529-008-9786-2

Cited by 156 publications

(86 citation statements)

References 19 publications

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“…Several animal studies have reported that dietary intake of silver nanoparticles caused lymphocyte infiltration, pigmentation of villi, discharge of mucus granules, and an abnormal mucus composition in the intestine. [21][22][23][24] Animal studies have reported that silver nanoparticles can accumulate in various organs after ingestion, including the liver, kidneys, spleen, stomach, and small intestine. 15,25,26 These results suggest that silver nanoparticles can be absorbed by the GIT into the systemic circulation, and then be distributed throughout various organs.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

2017

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Nanotechnology offers the food industry a number of new approaches for improving the quality, shelf life, safety, and healthiness of foods. Nevertheless, there is concern from consumers, regulatory agencies, and the food industry about potential adverse effects (toxicity) associated with the application of nanotechnology in foods. In particular, there is concern about the direct incorporation of engineered nanoparticles into foods, such as those used as delivery systems for colors, flavors, preservatives, nutrients, and nutraceuticals, or those used to modify the optical, rheological, or flow properties of foods or food packaging. This review article summarizes the application of both inorganic (silver, iron oxide, titanium dioxide, silicon dioxide, and zinc oxide) and organic (lipid, protein, and carbohydrate) nanoparticles in foods, highlights the most important nanoparticle characteristics that influence their behavior, discusses the importance of food matrix and gastrointestinal tract effects on nanoparticle properties, emphasizes potential toxicity mechanisms of different food-grade nanoparticles, and stresses important areas where research is still needed. The authors note that nanoparticles are already present in many natural and processed foods, and that new kinds of nanoparticles may be utilized as functional ingredients by the food industry in the future. Many of these nanoparticles are unlikely to have adverse affects on human health, but there is evidence that some of them could have harmful effects and that future studies are required.

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Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

2017

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“…Due to its antimicrobial properties, it is used in many domains including medicine (Dongwei et al, 2009). However, silver ion (Ag + ) is cytotoxic (Cha et al, 2008) and its utilization as a feed additive requires a chelation consisting in reducing positive charge (Ag + ) to non charge (Ag 0 ) molecule.…”

Section: Introductionmentioning

confidence: 99%

Chelating effect of silver nitrate by chitosan on its toxicity and growth performance in broiler chickens

et al. 2017

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Objective: This study was conducted to investigate the chelating effect of silver nitrate (AgNO3) by chitosan on growth performances, hematological and biochemical parameters, and the histopathological structure of the liver and the kidney in broiler chicken. Materials and methods: A total of 192 day-old Cobb 500 strain chicks were randomly assigned to 3 treatments of 64 chicks each. Control group was fed on basal diet without supplement (R0) and the two others groups were fed on rations supplemented with 10 mg of unchelated (RAg) or chelated (RCs-Ag) AgNO3 per Kg of feed, respectively. Parameters that have been studied consisted of feed intake, weight gain, blood and serum biochemical, and histopathological analyses of liver and kidney. Results: Results revealed that chelation of AgNO3 by chitosan did not have any effect on growth performances and hematological parameters in chicken. However, chelated and unchelated AgNO3 increased the serum content in triglyceride, and cholesterol and decreased the serum content in creatinin, albumin and alanine aminotransferase (ALAT). Chelating AgNO3 with chitosan prevented and corrected the toxicity induced on the histological structure of liver and kidney. Conclusion: Chitosan can be used as a chelating agent to alleviate the harmful effects of AgNO3 as silver ion for poultry.

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“…12,13 AgNPs were found in various body tissues, including the blood, brain, liver, spleen, kidneys, thymus, lungs, and heart. [14][15][16][17][18][19] Previous studies have demonstrated that AgNPs can exert cytotoxic and genotoxic effects both in vitro (human cell lines [20][21][22][23][24][25] ) and in vivo (mice 26,17 and fish 27,28 ). Oxidative stress can strongly affect testicular functions important for spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

Zhang¹,

Choi²,

Han³

et al. 2015

IJN

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Background Silver nanoparticles (AgNPs) possess unique physical, chemical, and biological properties. AgNPs have been increasingly used as anticancer, antiangiogenic, and antibacterial agents for the treatment of bacterial infections in open wounds as well as in ointments, bandages, and wound dressings. The present study aimed to investigate the effects of two different sizes of AgNPs (10 nm and 20 nm) in male somatic Leydig (TM3) and Sertoli (TM4) cells and spermatogonial stem cells (SSCs). Methods Here, we demonstrate a green and simple method for the synthesis of AgNPs using Bacillus cereus culture supernatants. The synthesized AgNPs were characterized using ultraviolet and visible absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). The toxicity of the synthesized AgNPs was evaluated by the effects on cell viability, metabolic activity, oxidative stress, apoptosis, and expression of genes encoding steroidogenic and tight junction proteins. Results AgNPs inhibited the viability and proliferation of TM3 and TM4 cells in a dose- and size-dependent manner by damaging cell membranes and inducing the generation of reactive oxygen species, which in turn affected SSC growth on TM3 and TM4 as feeder cells. Small AgNPs (10 nm) were more cytotoxic than medium-sized nanoparticles (20 nm). TEM revealed the presence of AgNPs in the cell cytoplasm and nucleus, and detected mitochondrial damage and enhanced formation of autosomes and autolysosomes in the AgNP-treated cells. Flow cytometry analysis using Annexin V/propidium iodide staining showed massive cell death by apoptosis or necrosis. Real-time polymerase chain reaction and western blot analyses indicated that in TM3 and TM4 cells, AgNPs activated the p53, p38, and pErk1/2 signaling pathways and significantly downregulated the expression of genes related to testosterone synthesis (TM3) and tight junctions (TM4). Furthermore, the exposure of TM3 and TM4 cells to AgNPs inhibited proliferation and self-renewal of SSCs. Conclusion Our results suggest that AgNPs exhibit size-dependent nanoreprotoxicity in male somatic cells and SSCs, strongly suggesting that applications of AgNPs in commercial products must be carefully evaluated. Further studies of AgNPs-induced nanoreprotoxicity in animal models are required.

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Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles

Cited by 156 publications

References 19 publications

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

Chelating effect of silver nitrate by chitosan on its toxicity and growth performance in broiler chickens

Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

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