Comparison of acute toxicities of deoxynivalenol (vomitoxin) and 15-acetyldeoxynivalenol in the B6C3F1 mouse

Forsell, James H.; Jensen, Richard K.; Tai, J.-H.; Witt, Mary F.; Lin, Wen; Pestka, James J.

doi:10.1016/0278-6915(87)90149-9

Cited by 90 publications

(40 citation statements)

References 15 publications

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“…However, it has to be pointed out that the intended intraperitoneal infusion of DON for 7 days looks more like one single dose, as 12 h after pump implantation plasma DON concentrations were zero or only marginally higher (Fig. 1) (Forsell et al 1987), or by the lowest observed effect level for emetic properties in pigs of 0.1 mg DON/kg bw after oral and 0.05 mg DON/kg bw after intraperitoneal administration (Forsyth et al 1977). The question why the DON release by the osmotic minipump was not as expected cannot be solved from the present results.…”

Section: Discussionmentioning

confidence: 97%

On the effects of the Fusarium toxin deoxynivalenol (DON) administered per os or intraperitoneal infusion to sows during days 63 to 70 of gestation

et al. 2010

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Six pregnant sows of 180.6 ± 5.6 kg were fed either a Fusarium-contaminated (4.42 mg DON and 48.3 µg ZON per kg, DON per os, n = 3) or a control diet (0.15 mg DON and 5 µg ZON/kg) in the period of days 63 and 70 of gestation. On day 63 of gestation, sows fed the control diet were implanted with an intraperitoneal osmotic minipump (delivery rate of 10 µL/h, for 7 days) containing 50 mg pure (98%) DON in 2 ml 50% DMSO (DON ip, n = 3). Frequent plasma samples were taken to estimate the kinetics after oral and ip DON exposure. The intended continuous delivery of DON by the intraperitoneal minipump could not be shown, as there was a plasma peak (Cmax) of 4.2-6.4 ng DON/mL either immediately (sow IP-2+3) or 2.5 h (sow IP-1) after implantation of the pump followed by a one-exponential decline with a mean half-time (t1/2) of 1.75-4.0 h and only negligible DON plasma concentrations after 12 h. Therefore, the DON ip exposure has to be regarded as one single dose 1 week before termination of experiment. The DON per os sows showed a mean basis level (after achieving a steady state) of DON plasma concentration of about 6-8 ng/mL, as also indicated by the plasma DON concentration at the termination of the experiment. On day 70, caesarean section was carried out, the fetuses were killed immediately after birth, and samples of plasma, urine, and bile were taken to analyze the concentration of DON and its metabolite de-epoxy-DON. At necropsy there were no macroscopic lesions observed in any organ of either sows or piglets. Histopathological evaluation of sows liver and spleen revealed no alterations. The proliferation rate of peripheral blood mononuclear cells (PBMC) with or without stimulation was not affected by the kind of DON treatment. The exposure of pregnant sows at mid-gestation (days 63-70, period of organogenesis) to a Fusarium toxin-contaminated diet (4.42 mg DON and 0.048 mg ZON per kg) or pure DON via intraperitoneal osmotic minipump did not cause adverse effects on health, fertility, maintenance of pregnancy, and performance of sows and their fetuses. However, DON was detected in fetus plasma, indicating that this toxin can pass the placental barrier and may cause changes in the proportion of white blood cells (lower monocyte and neutrophil and higher lymphocyte proportion in DON per os fetuses).

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Section: Discussionmentioning

confidence: 97%

On the effects of the Fusarium toxin deoxynivalenol (DON) administered per os or intraperitoneal infusion to sows during days 63 to 70 of gestation

et al. 2010

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“…Data on the relative toxicity of the monoacetylated forms of DON in mammals are limited and somewhat contradictory in that 3ADON was more than twice as toxic to mice via intraperitoneal injection as 15ADON while oral toxicity values for these trichothecenes were identical (Forsell et al, 1987;Morooka, 1973, 1974). 3ADON, but not 15ADON, also was found to be resistant to detoxification by rumen microorganisms (King et al, 1984).…”

Section: Discussionmentioning

confidence: 98%

An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America

Ward¹,

Clear²,

Rooney³

et al. 2008

Fungal Genetics and Biology

437

424

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“…The toxicity of DON is attributed to the inhibition of both protein biosynthesis as well as DNAand RNA-synthesis at the ribosomal level (Rotter et al 1996;SCF 1999). The oral LD 50 values of DON are 78 mg/kg bodyweight (bw) in B6C3F1 mice (Forsell et al 1987) and 46 mg/kg in DDY mice (Yoshizawa and Morooka 1974). Pigs are especially sensitive to DON in the diet.…”

Section: Introductionmentioning

confidence: 99%

Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry

et al. 2010

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Deoxynivalenol (DON) is one of most widespread mycotoxins in cereal commodities, and animal feed is prevalently contaminated at high concentrations. This poses a problem in animal nutrition as especially pigs are very sensitive to DON. An effective process for the reduction of the DON concentration is the treatment of contaminated feed with sodium bisulfite (SBS) whereby DON is transformed into DON-sulfonate (DONS). Although the success of this treatment has been confirmed in several feeding studies, it is unexplained if the decrease of DON is accompanied with a coincident increase of DONS. For this reason, we developed a method for the analysis of DONS using hydrophilic interaction chromatography coupled to tandem mass spectrometry. In order to investigate the correlation between DON and DONS concentrations during SBS-treatment, DON-contaminated wheat was treated with SBS and stored for up to 36 days. At defined timepoints of this treatment, samples were analyzed for DON and DONS using stable isotope labeled standards. The preparation, purification, and structure elucidation of DONS, and the HILIC-HPLC-MS/MS method for the analysis of DONS as well as the results of two storage experiments are presented in this paper.

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Comparison of acute toxicities of deoxynivalenol (vomitoxin) and 15-acetyldeoxynivalenol in the B6C3F1 mouse

Cited by 90 publications

References 15 publications

On the effects of the Fusarium toxin deoxynivalenol (DON) administered per os or intraperitoneal infusion to sows during days 63 to 70 of gestation

On the effects of the Fusarium toxin deoxynivalenol (DON) administered per os or intraperitoneal infusion to sows during days 63 to 70 of gestation

An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America

Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry

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