Comparison of additive effects on the PVA/starch cryogels: Synthesis, characterization, cytotoxicity, and genotoxicity studies

Ceylan, Seda; Göktürk, Dilek; Demir, Didem; Özdemir, Meryem Damla; Bölgen, Nimet

doi:10.1080/00914037.2017.1383254

Cited by 19 publications

(19 citation statements)

References 31 publications

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“…After the incubation process, cells were harvested and DNA isolations were obtained. In the last step; isolated DNAs were loaded in the agarose gel electrophoresis for the investigation of DNA fragmentation (15).…”

Section: Genotoxicity Assaymentioning

confidence: 99%

“…PVA is a biodegradable polymer used in tissue engineering due to its biodegradability, crystallinity, and mechanical properties. PVA can be blended with starch, gelatin, polycaprolactone (PCL), and CHI to increase its biodegradability and biocompatibility (13)(14)(15)(16). CHI is a non-toxic, biodegradable, and biocompatible polymer and has been extensively studied in the design of many different types of tissue engineering applications (17).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations

Ceylan

Alatepeli

2021

Journal of the Turkish Chemical Society Section A: Chemistry

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Cryogelation has become an advantageous method to obtain macro-porous materials with welldefined, interconnected pores for tissue engineering applications. Herein, polyvinyl alcohol and chitosan polymers (PVA-CHI) were used to produce cryogel scaffolds via cryogelation. Glutaraldehyde was used as a crosslinking agent and the effect of crosslinking amount on the properties of scaffolds investigated. Glutaraldehyde amount was divided into 5, 10, and 15% total amount of polymer concentration. The optimized pore morphology was obtained as a scaffold containing 5% glutaraldehyde amount. In addition to the FTIR, SEM, swelling, and degradation analyses, mechanical tests were performed to present the characterization properties of the cryogels. Direct and indirect cytotoxicity test and genotoxicity experiments were performed with Mouse Embryonic Fibroblasts (MEF). In addition, cell morphologies on scaffolds were analyzed with SEM. The results showed that PVA-CHI based cryogels had no genotoxic and cytotoxic effects on MEF cells and have a potential for tissue engineering applications.

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Section: Genotoxicity Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations

Ceylan

Alatepeli

2021

Journal of the Turkish Chemical Society Section A: Chemistry

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“…PVA/Starch cryogels were prepared by cryogelation technique as described in our previous study [13]. Briefly, PVA/Starch solution was fixed at 8% (w/v) polymer concentration at 90:10 (w:w) polymer ratio.…”

Section: Preparation Of Pva/starch Cryogel Scaffoldmentioning

confidence: 99%

“…SDS was added to the prepared polymer solution and homogeneous mixture was incubated at cryostat and freezer, then lyophilized before use. Synthesis, characterization, cytotoxicity and genotoxicity evaluation of PVA/Starch cryogels were investigated in previous study [13].…”

Section: Preparation Of Pva/starch Cryogel Scaffoldmentioning

confidence: 99%

“…3-(4,5-dimethylthiazoyl-2yl)-2,5-diphenyltetrazolium bromide assay and SEM were used to investigate the biocompatibility of the scaffolds and cell morphology. In order to evaluate DNA fragmentation, genotoxicity test was also performed [13]. They were found to be biocompatible and have ability to enhance the attachment and growth of Mouse Embryonic Fibroblast cells.…”

Section: Synthesis and Characterization Of Agnpsmentioning

confidence: 99%

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Green synthesized silver nanoparticles loaded PVA/Starch cryogel scaffolds with antibacterial properties

Demir

Ceylan

Gül

et al. 2019

Teh. glas. (Online)

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In this study, Polyvinyl alcohol/Starch (PVA/Starch) cryogel scaffolds were combined with antibacterial silver nanoparticles (AgNPs), and the antimicrobial properties of composite scaffolds were determined for potential in tissue engineering applications. The porous PVA/Starch scaffolds were prepared by cryogelation technique. The nanoparticles were prepared by green synthesis from Aloe barbadensis leaf extract and characterized. The antibacterial, antifungal and antiyeast properties of AgNPs and AgNPs loaded PVA/Starch cryogel scaffolds were investigated. The highest antimicrobial activity of composite scaffold was found against Pseudomonas aeruginosa. Based on our studies, the results indicate that biodegradable, biocompatible and antimicrobial AgNPs loaded PVA/Starch scaffolds have potential to be used at an infection site in tissue engineering applications.

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Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

Öfkeli

Demir

Bölgen

2020

J of Applied Polymer Sci

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The objective of this study is to develop biomimetic chitosan: gelatin (CH:Gel) cryogels for bone tissue engineering, combining the biological recognition of natural polymers with the distinguished interconnected porosity of cryogels, and biomimicking properties of bone like hydroxyapatite. The control of the biomineralization process onto biomaterials should be evaluated before clinical application. Therefore, the effect of chitosan and gelatin ratios on the final properties of the cryogels were investigated. FTIR, XRD, and SEM analysis indicated that the SBF coating exhibited similar characteristics to hydroxyapatite. The cryogels showed good biocompatibility with L929 mouse fibroblasts. Clinical outcomes and gross pathological examination showed that neither necrosis nor foreign body reaction was noted at the end of implantation. The biomimetically mineralized scaffold was found to be non‐irritant and non‐toxic for bone tissue. The biological performance and favorable properties demonstrated that the SBF coated CH:Gel cryogel can be a promising biomimetic scaffold for bone tissue engineering applications.

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Comparison of additive effects on the PVA/starch cryogels: Synthesis, characterization, cytotoxicity, and genotoxicity studies

Cited by 19 publications

References 31 publications

Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations

Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations

Green synthesized silver nanoparticles loaded PVA/Starch cryogel scaffolds with antibacterial properties

Biomimetic mineralization of chitosan/gelatin cryogels and in vivo biocompatibility assessments for bone tissue engineering

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