Comparison of adherence, cytotoxicity, and Gal/GalNAc lectin gene structure in Entamoeba histolytica and Entamoeba dispar

Dodson, James M.; Clark, C Graham; Lockhart, Lauren A.; Leo, Brian M.; Schroeder, James W.; Mann, Barbara J.

doi:10.1016/s1383-5769(97)00032-9

Cited by 35 publications

(23 citation statements)

References 31 publications

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“…Evidence for the participation of this molecule in the adhesion event of the parasite has been demonstrated by reduced amebic adherence to human erythrocytes, neutrophils, colonic mucins, and epithelia and to certain bacteria when the lectin is inhibited by galactose (17,29,33,124). Complete inhibition of E. histolytica adherence to target cells or colonic mucins is not observed, even when the lectin is blocked with high (100 to 500 mM) concentrations of galactose or GalNAc monomers (42,77,115). It has been suggested that the spacing of multiple GalNAc residues on the surface of target cells is important for optimal lectin binding (1).…”

Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 99%

“…Comparison between E. histolytica and E. dispar reveals very similar Gal-GalNAc adhesins that efficiently bind to target cells and colonic mucins (29,42,119). Derived amino acid sequences encoded by homologous genes show that E. dispar heavy-and light-subunit clones are 86 and 79% identical in their primary structures, respectively, to their E. histolytica counterparts (42,119).…”

Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 99%

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Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Espinosa‐Cantellano¹,

Martı́nez-Palomo²

2000

Clinical Microbiology Reviews

191

115

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Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 99%

Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 99%

Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Espinosa‐Cantellano¹,

Martı́nez-Palomo²

2000

Clinical Microbiology Reviews

191

115

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“…Acute dysentery, intestinal amoebiasis: The disease begins when trophozoites of E. histolytica are adhered to the epithelial cells of the colon with a special Gal/GalNAc lectin, a heterodimer comprised of three subunits with a total molecular weight 260 kDa [21][22]. Stuck to the trophozoites mucosa secrete specific proteolytic enzymes such as hyaluronidase, cysteine proteinase, cathepsin B, which produce a local inflammatory reaction, congestion, degradation attacked by amoeba cells and facilitate trophozoites further invasion of the intestinal submucosa tissue [13,18,21,23].…”

Section: Pathophysiology and Clinical Signs Of Infestationmentioning

confidence: 99%

“…Stuck to the trophozoites mucosa secrete specific proteolytic enzymes such as hyaluronidase, cysteine proteinase, cathepsin B, which produce a local inflammatory reaction, congestion, degradation attacked by amoeba cells and facilitate trophozoites further invasion of the intestinal submucosa tissue [13,18,21,23]. Trophozoites of amoeba cause induction of the enzyme cyclooxygenase-2 (COX-2) in the lining of the bowel, leading to an increase in the secretion of prostaglandin E 2 (PGE 2 ), which contributes to the stimulation of the inflammatory process [24].…”

Section: Pathophysiology and Clinical Signs Of Infestationmentioning

confidence: 99%

Entamoeba histolytica - Pathogenic Protozoan of the Large Intestine in Humans

Nowak¹

2015

J Clin Microbiol Biochem Technol

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Entamoeba histolytica is a cosmopolitan, parasitic protozoan of human large intestine, which is a causative agent of amoebiasis. Amoebiasis manifests with persistent diarrhea containing mucus or blood, accompanied by abdominal pain, flatulence, nausea and fever. In some cases amoebas may travel through the bloodstream from the intestine to the liver or to other organs, causing multiple abscesses. Amoebiasis is a dangerous, parasitic disease and after malaria the second cause of deaths related to parasitic infections worldwide. The highest rate of infections is observed among people living in or traveling through the tropics. Laboratory diagnosis of amoebiasis is quite difficult, comprising of microscopy and methods of molecular biology. Pathogenic species Entamoeba histolytica has to be differentiated from other nonpathogenic amoebas of the intestine, so called commensals, that very often live in the human large intestine and remain harmless. Other intestinal commensals are Entamoeba dispar and Entamoeba moshkovskii, morphologically the same as pathogenic species Entamoeba histolytica sensu stricto. The differential diagnosis of these three amoebas is possible with detection of their DNA.get sick, and 100 thousand people die because of amoebosis-induced infection dysentery [2].E. histolytica belongs to the cosmopolitan parasites, occurring throughout the globe. Particularly exposed to this parasite are people living and traveling to the tropical and subtropical zones (Asia, Africa, India, Indonesia, Mexico, South America, South Africa). These zones climatic conditions are optimal for the Protozoan cysts, which described the external environment can survive for many days. This fact contributes to the increase in the number of infections slider Entamoeba histolytica amongst people in tropical zones. According to Weinke to high-risk groups who are particularly vulnerable to being infected by E. histolytica include men with a homosexual orientation [3]. Prevalence, it means the incidence of infection is low in Poland and is about 1%, while in tropical countries can claim up to 50%. Most of the amoebiasis cases reported in Poland applies to people returning from a different climate zone and foreigners.The digestive tract may be inhabited by several species of nonpathogenic amoebas in the family Entamoebidae, which include Entamoeba coli, Entamoeba hartmanni, Entamoeba polecki, Endolimax nana,. The aforementioned amoebas are so called commensals of colon, which may settle the intestinal mucosa physiologically not attracting damages. There are only isolated reports in the literature that some commensals may lead to pathogenic actions in the human large intestine. There have been a couple of cases of diarrhea problems caused by infections of Endolimax nana, Entamoeba polecki and I. bűtschlii in immunocompromised patients [6].In 1925, Brumpt suggested that within the family Entamoebidae there are species Entamoeba dispar, which is morphologically Abbreviations E.

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“…This lectin, consisting of heavy (170-kDa) and light (31-or 35-kDa) subunits linked by disulfide bonds, is antigenically conserved (20,21). Although there are shared epitopes between the lectins of E. histolytica and E. dispar, there is only 77 to 85% DNA sequence identity between these molecules (8,22,23). In this study, we generated monoclonal antibodies (MAbs) by immunizing mice with a recombinant heavy lectin subunit that had been fixed in the stool preservative, sodium acetate-acetic acid-formalin (SAF).…”

mentioning

confidence: 99%