Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants

Singh, B. N.; Kaur, Amritpal

doi:10.1007/s12892-011-0037-z

Cited by 11 publications

(4 citation statements)

References 21 publications

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“…Whenever the compounds supplemented to these basal media did not have the appropriate results, the researchers used some other compounds such as coconut milk, malt extract, tomato juice, yeast extract, or orange juice, which gave them optimal results (Molnár et al, 2011). Singh and Kaur (2011) revealed that the application of malt extract did not cause significant differences in proliferation percentage, but they obtained the longest branches as compared to the plants exposed to cytokinin treatments. Khorsha et al (2016) reported that the number of shoots and intermodal length of grapevines plantlets were influenced in optimized medium enriched with apricot gum.…”

Section: Resultsmentioning

confidence: 88%

In vitro responses of raspberry plantlets to the culture media enriched with some plant sprout powders

Alizadeh¹,

Dabbagh²,

Badeli³

et al. 2023

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The growth and morphogenesis responses of cultured cell and plant tissues can be improved by addition of small amounts of some organic materials like plant sprout powders (PSP). A preliminary experiment was undertaken to examine the response of raspberry plantlets to media enriched with plant sprout powders derived from wheat, vetch and alfalfa plant species. The Murashige and Skoog (MS) medium supplemented with IBA (2.0 mg/l) and activated charcoal (200 mg/l) was exploited as optimized shoot proliferation for raspberry. Seeds of wheat, vetch or alfalfa were subjected to germination and when the rootlets attained 1 cm long, the sprouts were dried and ground to provide a homogenous powder. The shoot proliferation medium already optimized for raspberry was supplemented with 1.0 g/l of each wheat, vetch, or alfalfa sprout powders. The double-node cuttings of in vitro grown raspberries were inoculated on these media. Therefore, the growth responses of raspberries were measured in media with or without PSP. The results revealed the positive response of raspberry plantlets to the medium containing vetch sprout powder and most of the growth parameters including number of shoots, leaves, roots, and plantlet length were improved. Besides obvious apparent quality of in vitro cultures and better growth performance, the plantlets grown on media enriched with vetch sprout powder showed highest leaf number (6.41) and longer internodal length (1.89 mm). Therefore, the obtained data encourage the utilization of PSP in plant tissue culture media.

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Section: Resultsmentioning

confidence: 88%

In vitro responses of raspberry plantlets to the culture media enriched with some plant sprout powders

Alizadeh¹,

Dabbagh²,

Badeli³

et al. 2023

ijmfmap

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“…Furthermore, the combination of three hormones namely BAP, kinetin, NAA leads to a better leaf production for of mandarin variety [50]. Also, the medium which contains GA3 is the one which stimulate root growth [41], besides, Singh and Kaur [51] showed that the use of auxins is so important for root system for citrus species such as limonia, sinensis and jambhiri citrus.…”

Section: Results Of Flow Cytometry Analysismentioning

confidence: 99%

In Vitro Development and Germination of Immature Embryos of Sweet Oranges Parson Brown and Pineapple (Citrus sinensis)

Mahmoudi¹,

Handaji²,

Ibriz³

et al. 2018

JAST-B

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Oranges are classified as the most traded fruit variety worldwide. The diversification of the varietal profile of this group is among the objectives of the Citrus breeding program. So, the creation of new triploid hybrids via immature embryos rescue, can lead to an increase in gene pool and produce seedless varieties. In addition, flow cytometry was successfully used for analyzing ploidy levels and genetic origin of triploid plants. The present study was aimed at optimizing immature embryos in vitro germination and plantlet development based on medium chemical composition of the two orange varieties, Pineapple and Parson Brown. When the fruit gets matured, they are harvested and the seeds extracted are classified according to their size. Only those which are small are cultured in a sterile Murashige and Tucker (MT) basal medium. The different concentrations of growth regulators are put to the test in order to gain the best medium for seeds development. M1 (MT + 1 mg/L gibberellic acid (GA3)), M2 (MT + 1 mg/L kenitin + 0.5 mg/L 6-benzylaminopurine (BAP) + 0.1 naphthalene acetic acid (NAA)), M3 (MT + 25 mg/L adenine sulfate (ADS)), M4 (MT + 0.5 mg/L kenitin + 0.5 mg/L BAP + 1 mg/L GA3). Percentage of germinated embryos (PGE) is the highest in M3 with 100% and 90% for both Pineapple and Parson Brown varieties, respectively. In addition, the germination interval average (GIA) is shorter in M1 for both varieties, which takes 6 d and 7 d, respectively. As regard to speed seedling growth (mm/week), both varieties undergo significant change in all four mediums. Similarly, the highest rate of acclimatization for the Pineapple and Parson Brown varieties is 80% and 90% in M1. In general, the smaller the embryos are, the more sensitive they are to culture medium composition. It is therefore essential to optimize the medium components in order to stimulate their in vitro development. Therefore, M1 (MT + 1 mg/L GA3) remains the best to promote short term good germination and produce a better rate of acclimatization.

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“…In addition, this technique can also produce crops on a relatively large scale in comparison with traditional plant breeding. Furthermore, in vitro cultures eliminate infections and can be faster than traditional plant cultures (Savita et al, 2011;Singh and Kaur, 2011).…”

Section: Techniques Of Plant Tissue Culture Micropropagationmentioning

confidence: 99%

Citrus: A Need for its Conservation in Utilising its Medicinal Values through Biotechnological Tools

Roy¹,

Roy²,

Sundarrao³

2020

Int.J.Curr.Microbiol.App.Sci

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Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants

Cited by 11 publications

References 21 publications

In vitro responses of raspberry plantlets to the culture media enriched with some plant sprout powders

In vitro responses of raspberry plantlets to the culture media enriched with some plant sprout powders

In Vitro Development and Germination of Immature Embryos of Sweet Oranges Parson Brown and Pineapple (Citrus sinensis)

Citrus: A Need for its Conservation in Utilising its Medicinal Values through Biotechnological Tools

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