Comparison of amino-acid compositions and E4/E6 ratios of soil and water humic substances fractions obtained by polyacrylamide gel electrophoresis

Summary -ZusammenfassungHumic acids (HAS) from four soils were fractionated by size exclusion chromatography (SEC) on Sephadex (3-75. Three fractions were obtained in all humic acids, collected and assayed by polyacrylamide gel electrophoresis (PAGE). The unfractionated HA from each soil was used as reference. Each chromatographic fraction formed one electrophoretic zone corresponding closely to one band of the reference sample with some admixture of the fractions preceding or following. The results indicate that fractionation of HAS by tandem SEC-PAGE can be successfully used for obtaining fractions of reduced polydispersity and different electrophoretic mobilities. Pyrolysislmethylationgas chromatography-mass spectrometry of the full size preparations of HA and fractions with exactly defined molecular size and electrophoretic mobility showed a different distribution in humic components, mainly lipids, lignin derivatives and N-containing compounds.

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Tandem size exclusion chromatography‐Polyacrylamide gel electrophoresis of humic acids

Trubetskoj

Trubetskaya

Afanasieva

et al. 1998

J Plant Nutrition & Soil

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Seasonal variability of a conditional stability constant and the characterization of sedimentary humic substances from typical agricultural and urban areas

et al. 2013

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Origin and structures of groundwater humic substances from three Danish aquifers

Grøn

Wassenaar

Krog

1996

Environment International

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Comparison of amino-acid compositions and E4/E6 ratios of soil and water humic substances fractions obtained by polyacrylamide gel electrophoresis

Cited by 7 publications

References 9 publications

Tandem size exclusion chromatography‐Polyacrylamide gel electrophoresis of humic acids

Tandem size exclusion chromatography‐Polyacrylamide gel electrophoresis of humic acids

Seasonal variability of a conditional stability constant and the characterization of sedimentary humic substances from typical agricultural and urban areas

Origin and structures of groundwater humic substances from three Danish aquifers

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