Comparison of an in vitro skin model to normal human skin for dermatological research

Monteiro‐Riviere, Nancy A.; Inman, Alfred O.; Snider, Thomas H.; Blank, Jim A.; Hobson, David W.

doi:10.1002/(sici)1097-0029(19970501)37:3<172::aid-jemt2>3.0.co;2-q

Cited by 59 publications

(39 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reconstructed tisThis paper was submitted directly (Track II) to the PNAS office. sues show lipid profiles similar to the corresponding tissue in vivo, release the relevant cytokines, and demonstrate the presence of gap junctions (21)(22)(23)(24).…”

Section: Methodsmentioning

confidence: 87%

“…Analysis of the tissue microstructure has demonstrated the presence of keratohyalin granules, tonofilament bundles, desmosomes, and a multilayered stratum corneum containing intercellular lamellar lipid layers arranged in patterns characteristic of the in vivo epidermis (22). The reconstructed tissues are mitotically and metabolically active, maintaining the same differentiation patterns as those in vivo (21,23).…”

Section: Methodsmentioning

confidence: 96%

See 1 more Smart Citation

Biological effects in unirradiated human tissue induced by radiation damage up to 1 mm away

Belyakov

Mitchell

Parikh

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

272

166

View full text Add to dashboard Cite

A central tenet in understanding the biological effects of ionizing radiation has been that the initially affected cells were directly damaged by the radiation. By contrast, evidence has emerged concerning ''bystander'' responses involving damage to nearby cells that were not themselves directly traversed by the radiation. These long-range effects are of interest both mechanistically and for assessing risks from low-dose exposures, where only a small proportion of cells are directly hit. Bystander effects have been observed largely by using single-cell in vitro systems that do not have realistic multicellular morphology; no studies have as yet been reported in three-dimensional, normal human tissue. Given that the bystander phenomenon must involve cell-to-cell interactions, the relevance of such single-cell in vitro studies is questionable, and thus the significance of bystander responses for human health has remained unclear. Here, we describe bystander responses in a three-dimensional, normal human-tissue system. Endpoints were induction of micronucleated and apoptotic cells. A charged-particle microbeam was used, allowing irradiation of cells in defined locations in the tissue yet guaranteeing that no cells located more than a few micrometers away receive any radiation exposure. Unirradiated cells up to 1 mm distant from irradiated cells showed a significant enhancement in effect over background, with an average increase in effect of 1.7-fold for micronuclei and 2.8-fold for apoptosis. The surprisingly long range of bystander signals in human tissue suggests that bystander responses may be important in extrapolating radiation risk estimates from epidemiologically accessible doses down to very low doses where nonhit bystander cells will predominate.bystander ͉ normal human tissue ͉ radiological risk

show abstract

Section: Methodsmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 96%

Biological effects in unirradiated human tissue induced by radiation damage up to 1 mm away

Belyakov

Mitchell

Parikh

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

272

166

View full text Add to dashboard Cite

show abstract

“…Notably these structures were not observed in the rSE model in our previous study [27]. Moreover, specific ultrastructures in the layers, such as cuboidal basal cell shape, desmosomes, keratohyaline granules and CCE, were equally expressed in the grafted LCE cells and in the grafted keratinocytes [39][40][41][42][43][44]. However, a thinner CCE was observed in grafted UCE cells, which is consistent with the findings in morphological studies of haematoxylin and eosin sections at 2 weeks (Fig.…”

Section: Discussionmentioning

confidence: 96%

Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite

Sanmano¹,

Mizoguchi²,

Suga³

et al. 2005

Journal of Dermatological Science

View full text Add to dashboard Cite

“…This is caused by the loss of blood supply after surgical excision, hypoxia, and the accumulation of toxic metabolites. 8 Methods commonly used to assess the viability of ex vivo skin include the morphology of the tissue, 9 metabolic activity by MTT, [10][11][12] lactate dehydrogenase ͑LDH͒ activity assays, 13 skin pH, 14 and oxygen consumption. 11,15,16 Several studies have used the MTT assay to measure the decreasing viability of ex vivo skin.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the metabolic deterioration of ex vivo skin from ischemic necrosis through the imaging of intracellular NAD(P)H by multiphoton tomography and fluorescence lifetime imaging microscopy

et al. 2010

View full text Add to dashboard Cite

Abstract. Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be timeconsuming and provide limited metabolic information. Using multiphoton tomography and fluorescence lifetime imaging ͑MPT-FLIM͒ we assess ischemic necrosis of ex vivo skin by NAD͑P͒H autofluorescence intensity and fluorescence lifetime. Ex vivo skin is stored in the presence and absence of nutrient media ͑Dulbecco Modified Eagle Medium͒ at −20, 4, and 37°C and room temperature over a 7-day time course to establish different rates of metabolic deterioration. At higher temperatures we observe a decrease in NAD͑P͒H autofluorescence, higher image noise, and a significant increase in the average fluorescence lifetime ͑ m ͒ from ϳ1000 to 2000 ps. Additionally, significant distortions in NAD͑P͒H fluorescence lifetime histograms correspond to the reduction in autofluorescence. Skin kept at 4°C, with or without media, showed the least change. Our findings suggest that MPT-FLIM enables useful noninvasive optical biopsies to monitor the metabolic state and deterioration of human skin for research and clinical purposes.

show abstract

Comparison of an in vitro skin model to normal human skin for dermatological research

Cited by 59 publications

References 8 publications

Biological effects in unirradiated human tissue induced by radiation damage up to 1 mm away

Biological effects in unirradiated human tissue induced by radiation damage up to 1 mm away

Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite

Analysis of the metabolic deterioration of ex vivo skin from ischemic necrosis through the imaging of intracellular NAD(P)H by multiphoton tomography and fluorescence lifetime imaging microscopy

Contact Info

Product

Resources

About