Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite

Sanmano, B; Mizoguchi, Masayuki; Suga, Yasushi; Ikeda, Shigaku; Ogawa, Hideoki

doi:10.1016/j.jdermsci.2004.10.008

Cited by 26 publications

(20 citation statements)

References 48 publications

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“…As reported by other teams [Mizoguchi et al, 2000;Sanmano et al, 2005], the epithelial cells of the UC in monolayer cultures behaved very similarly to cultured keratinocytes [Pelletier et al, 1986]. Viable cells in primary culture formed compact, well-defined colonies of small, proliferative cells.…”

Section: Discussionsupporting

confidence: 64%

“…Other research groups have used single types of UC cells in tissue reconstruction, including pulmonary artery and cardiovascular conduits Kadner et al, 2002] as well as epithelial substitutes [Mizoguchi et al, 2004;Sanmano et al, 2005]. Interestingly, the UC stromal cells have been shown to exhibit a lack of alloreactivity in vitro [Ennis et al, 2008;Weiss et al, 2008].…”

Section: Discussionmentioning

confidence: 99%

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Harvesting the Potential of the Human Umbilical Cord: Isolation and Characterisation of Four Cell Types for Tissue Engineering Applications

Hayward

Fradette²,

Galbraith³

et al. 2012

Cells Tissues Organs

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The human umbilical cord (UC) has attracted interest as a source of cells for many research applications. UC solid tissues contain four cell types: epithelial, stromal, smooth muscle and endothelial cells. We have developed a unique protocol for the sequential extraction of all four cell types from a single UC, allowing tissue reconstruction using multiple cell types from the same source. By combining perfusion, immersion and explant techniques, all four cell types have been successfully expanded in monolayer cultures. We have also characterised epithelial and Wharton’s jelly cells (WJC) by immunolabelling of specific proteins. Epithelial cell yields averaged at 2.3 × 10⁵ cells per centimetre UC, and the cells expressed an unusual combination of keratins typical of simple, mucous and stratified epithelia. Stromal cells in the Wharton’s jelly expressed desmin, α-smooth muscle actin, elastin, keratins (K12, K16, K18 and K19), vimentin and collagens. Expression patterns in cultured cells resembled those found in situ except for basement membrane components and type III collagen. These stromal cells featured a sustained proliferation rate up to passage 12 after thawing. The mesenchymal stem cell (MSC) character of the WJC was confirmed by their expression of typical MSC surface markers and by adipogenic and osteogenic differentiation assays. To emphasise and demonstrate their potential for regenerative medicine, UC cell types were successfully used to produce human tissue-engineered constructs. Both bilayered stromal/epithelial and vascular substitutes were produced, establishing the versatility and importance of these cells for research and therapeutic applications.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Harvesting the Potential of the Human Umbilical Cord: Isolation and Characterisation of Four Cell Types for Tissue Engineering Applications

Hayward

Fradette²,

Galbraith³

et al. 2012

Cells Tissues Organs

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show abstract

“…It has been shown that the umbilical cord epithelium (UCE) expresses a cytokeratin pattern, which is similar to human epidermis [12], although the general architecture of the umbilical cord epithelium significantly differs from the mammalian epidermis [13]. Furthermore, epithelial cells derived from UCE are capable of forming a stratified epithelium when seeded on fibroblast populated collagen gels [14,15]. A very recent study also shows that primary neonatal keratinocytes and umbilical cord lining epithelial cells have a very similar pattern of cytokeratin CK1/CK10 and deltaNp63 expression [16].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Umbilical Cord Lining Cell with CD227 Positivity and Unique Pattern of P63 Expression and Function

Reza

Phan

et al. 2010

Stem Cell Rev and Rep

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Umbilical cord tissue is gaining attention as a novel source of multipotent stem cells because it is easily obtainable, ethically acceptable and the cells are immunologically naïve. In this study, we have isolated and characterized a new cell type expressing MUCIN1 (CD227) from human umbilical cord lining which we termed MUCIN-expressing Cord Lining Epithelial Cell (CLEC-muc). We found that CLEC-muc is highly proliferative and had significant clonogenic ability. These cells express embryonic stem cell markers OCT-4, NANOG, SSEA-4, REX1 and SOX2. Despite the abundant expression of epithelial cell marker MUCIN1 and cytokeratins, this population is also positive to the mesenchymal stem cell (MSC) marker CD166. CLEC-muc is unique in p63 expression that shuttles from the cytoplasm to the nucleus over time in culture. To understand p63 regulation and function in CLEC-muc, cells were treated with BMP4, a potent morphogen that plays a role in epidermal differentiation via p63 upregulation in ES cell and subsequent analyses were done. We found that BMP4 does not alter cytoplasmic expression of p63 that promotes cell proliferation. However, it increases nuclear p63 expression together with several other epithelial-associated genes such as GATA3, JAGGED1, NOTCH1, HES1 and IKKα. BMP4 has also been found to weakly induce deltaNp63 expression in CLEC-muc. Our results suggest that CLEC-muc is a novel stem cell-like population that can be further differentiated by BMP4 to generate specific cell-types probably destined to form non-keratinized epithelia.

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“…Recently it has been reported that the umbilical cord epithelium (UCE) expresses a cytokeratin pattern similar to human epidermis [1], although the general architecture of the umbilical cord epithelium significantly differs from the mammalian epidermis [2]. Furthermore, cells derived from UCE are capable of forming a stratified epithelium when seeded on fibroblast populated collagen gels [3,4]. We therefore asked whether the umbilical cord could possibly serve as an alternative source for primary neonatal keratinocytes and investigated the similarity of UCE cells and neonatal primary keratinocytes at the molecular and functional level.…”

mentioning

confidence: 99%

Common features of umbilical cord epithelial cells and epidermal keratinocytes

Ruetze¹,

Gallinat²,

Lim

et al. 2008

Journal of Dermatological Science

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Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite

Cited by 26 publications

References 48 publications

Harvesting the Potential of the Human Umbilical Cord: Isolation and Characterisation of Four Cell Types for Tissue Engineering Applications

Harvesting the Potential of the Human Umbilical Cord: Isolation and Characterisation of Four Cell Types for Tissue Engineering Applications

Characterization of a Novel Umbilical Cord Lining Cell with CD227 Positivity and Unique Pattern of P63 Expression and Function

Common features of umbilical cord epithelial cells and epidermal keratinocytes

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