Comparison of Antiallergic Drugs in an Experimental Model of Ocular Anaphylaxis

Abstract

Purpose: To compare the efficacy of antiallergic drugs in an experimental ocular anaphylaxis model. Materials and Methods: Fifty-two rats were divided into 8 groups. Dithiothreitol and ovalbumin (OVA) were applied to both eyes of previously OVA-inoculated rats in groups 1–5 and 7. In groups 1–5, antiallergic efficacy of the drugs (lodoxamide, sodium cromoglycate, levacobastine, cimetidine, and diclofenac sodium) instilled on the right eyes were compared with the buffer instilled on the left eyes, measuring the amount of extravasated Evans blue (EB) dye by spectrophotometric analysis. Group 6 served as a control of EB dye extravasations in nonimmunized eyes. Histopathologic examinations for anaphylaxis and controls were done in groups 7 and 8. Results: Only levacobastine and lodoxamide decreased vascular permeability at a significant ratio (46%, p < 0.01 and 31%, p < 0.05, respectively). Conclusion: Levacobastine and lodoxamide but none of the three other drugs mentioned above were found to be effective in decreasing vascular permeability.

Chitosan-based thermosensitive hydrogel as a promising ocular drug delivery system: Preparation, characterization, and in vivo evaluation

“…Animal models have been frequently used to determine in vivo efficacy of antihistamines. 28,52 – 54 Presently murine models of allergic conjunctivitis may be preferred for the investigation of the immunological basis of the diseases. The guinea pig model of ocular active anaphylaxis still presents several advantages in the evaluation of topical treatment.…”