Comparison of Artemisia annua Bioactivities between Traditional Medicine and Chemical Extracts

Nageeb, Ahmed; Altawashi, Azza; Emwas, Abdul‐Hamid; Al‐Talla, Zeyad A.; Al-Rifai, Nahla

doi:10.2174/157340720904140404151439

Cited by 33 publications

(37 citation statements)

References 32 publications

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“…Medicinal plants have been used to treat various human diseases and recently, there has been wide spread interest in botanical medicines (Nagreeb et al, 2013). According to the world health organization (WHO) more than 80% of the world population relies on traditional medicine for their primary health care needs (Vashit and Jundal, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial, free radical scavenging and cytotoxic activities of Khaya grandifoliola C.DC extracts

Opawale¹,

Onifade²,

Ogundare³

2016

MJM

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Aims: Khaya grandifoliola C.DC is a plant used locally in Nigeria ethno medicine for remedy of various disease conditions. However, there is little scientific evidence to support the therapeutic claims of the plant. Therefore, these investigations were conducted to determine the antimicrobial activity, antioxidant and cytotoxic potentials of the plant extracts. Methodology and results: In vitro antimicrobial activity of the leaf and stem bark extracts of K. grandifoliola against some human pathogens was done using agar diffusion method. The free radical scavenging activity and cytotoxic property of the plant materials were evaluated using 2, 2-diphenyl-1-pieryhydrazyl radical (DPPH) and brine shrimp lethality bioassay methods respectively. The yields of the plant material extracts ranged from 3.57±0.06 to 6.49±0.01% and 4.76±0.02 to 9.17±0.06% for the leaf and stem bark extracts respectively. The minimum inhibitory concentration (MIC) of KG-A and KG-E ranged from 2.5 to 200 mg/mL and recorded remarkable activity against the growth of Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Trichophyton rubrum and Aspergillus flavus. However, Strepcoccus pneumoniae, Staphylococcus epidermidis, Klebsiella pneumoniae, and T. rubrum were resistant to the KG-W. The plant extracts demonstrated high DPPH free radical scavenging activity when compared with ascorbic acid used as control in the assay and, also exhibited lethality against brine shrimp larvae with LC50 values ranging from: leaf extracts (0.67 to 1502 ppm) and stem bark extracts (0.91 to 1431 ppm). Conclusion, significance and impact of study: The results show that the KG-A and KG-E have great potentials as antimicrobial agent and may be used in the treatment of infectious diseases caused by the susceptible organisms.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial, free radical scavenging and cytotoxic activities of Khaya grandifoliola C.DC extracts

Opawale¹,

Onifade²,

Ogundare³

2016

MJM

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“…For a long time, the therapeutic properties of various medicinal plants have been used to treat various human diseases and recently, there has been widespread interest in botanical medicines (1,2). It has been estimated that between 60-90% of the population of developing countries use traditional and herbal medicines almost exclusively and consider them to be a normal part of primary healthcare (3).…”

Section: Introductionmentioning

confidence: 99%

“…Although in most cases their efficacy and mechanisms of action have not been tested scientifically, these simple medicinal preparations often mediate beneficial responses due to their active chemical constituents (4). Moreover, the search for plants with antimicrobial activity has grown in importance during the recent years, due to a growing concern about the increase in the rate of infection caused by antibiotic-resistant microorganisms (1)(2)(3)(4)(5). Although the advances brought by technology has made life easier for people, many are still looking for better alternatives such as medicinal plants.…”

Section: Introductionmentioning

confidence: 99%

Characterization of an Antimicrobial Extract from Elaeagnus angustifolia

Dehghan¹,

Soltani²,

Kalantar³

et al. 2014

Int J Enteric Pathog

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Background: According to ethnobotanical data, Elaeagnus angustifolia fruit has wound healing activity, anti-inflammatory effect and antifebrile prosperities. Objectives: This study was performed as to the best of our knowledge; there has been no scientific report on the characterization of antimicrobial effect of E. angustifolia extract. Materials and Methods: An aqueous extract of Elaeagnus angustifolia was prepared and antimicrobial activity tests were performed on various target cultures. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extract was done using the broth dilution technique. To characterize the extract, shelf life, thermal and pH stability, effects of detergents such as Tween 80, Tween 20, Triton X100, toluene and enzymes on the antimicrobial activity of Elaeagnus angustifolia extract, were examined. Results:The MIC values ranged from 7.5 to 0.1 mg/mL, showing maximum activity (1.62 mg/mL) against E. coli. Similarly, the MBC of the extract against E. coli was 1.62 mg/mL. Antimicrobial activity of the extract was relatively stable when kept in the refrigerator for 60 days. The antimicrobial activity of Elaeagnus angustifolia extract was absolutely stable at temperatures up to 700° C. After exposure of the Elaeagnus angustifolia extract to different pH solutions in the range of 4-10, almost 100% residual activity was found against E. coli at pH 4, 5, 6, and 7. Treatment of the extract with detergents, lipase and lysozyme eliminated its antimicrobial activity. Conclusions: Our study gives an indication of the presence of promising antimicrobial compounds and provides basic information about the nature of the Elaeagnus angustifolia extract. Future studies should elucidate the components responsible for antimicrobial activity of these extracts against target cultures.

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“…Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool allowing investigation of the chemical composition of a given sample [14][15][16]. Furthermore, NMR can be used for structural elucidation and to study molecular dynamics [17][18][19][20][21][22].…”

Section: Discussionmentioning

confidence: 99%

The Cytotoxic Effect of Small and Large Molecules of PMF Fraction Extracted from Camel Urine on Cancer Cells

Mahboub

Khorshid

Emwas

2015

BJMMR

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Aim of the work: Animal urine, including that of camels, has long been used for the therapeutic management of human ailments. In this study, we sought to characterize the cytotoxic properties of newly derived purified fractions from previously described camel urine extract (PMF) on various cancer cell lines. Methodology: Two new size dissimilar fractions of PMF (large and small) were obtained by fractionalizing PMF using 3kD and 50kD membrane filters. A SRB cytotoxicity assay of the PMF Mahboub et al.; BJMMR, 6(4): 384-396, 2015; Article no.BJMMR.2015.213 385 fractions was performed on cancer cell lines (A549, HCT116, HepG2, MCF-7, U251 and Hela) as well as normal cell lines (human fibroblast cell line and Vero). Results: This study showed that the newly derived and more purified fraction of PMF (new PMF) possesses effective and selective anti-cancer properties against several types of cancer cell lines. Conclusion: This study, as well as previous ones, suggests that camel urine extracts (old and new PMF) may provide newer therapeutic alternatives to clinically manage cancer patients. However, further studies are needed to verify these positive preliminary results. Original Research Article

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Comparison of Artemisia annua Bioactivities between Traditional Medicine and Chemical Extracts

Cited by 33 publications

References 32 publications

Antimicrobial, free radical scavenging and cytotoxic activities of Khaya grandifoliola C.DC extracts

Antimicrobial, free radical scavenging and cytotoxic activities of Khaya grandifoliola C.DC extracts

Characterization of an Antimicrobial Extract from Elaeagnus angustifolia

The Cytotoxic Effect of Small and Large Molecules of PMF Fraction Extracted from Camel Urine on Cancer Cells

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