Comparison of bacterial communities of biofilms formed on different membrane surfaces

Lee, Sang Hoon; Hong, Ted; Kim, Bongchul; Hong, Seungkwan; Park, Hee Deung

doi:10.1007/s11274-013-1460-8

Cited by 19 publications

(15 citation statements)

References 13 publications

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“…This difference was due to the difference in the assembly mechanism of AS and biofilm communities, which are two distinct forms of microbial aggregates. Similar results were reported in previous studies where the biofilm community was distinct from the AS community in lab- (Lim et al, 2004;Choi et al, 2006;Zhang et al, 2006;Huang et al, 2008;Fontanos et al, 2010;Lim et al, 2012;Piasecka et al, 2012;Lee et al, 2014), pilot-(Jinhua et al, 2006Miura et al, 2007) and full-scale (Jo et al, 2016) MBRs.…”

Section: Accepted Manuscriptsupporting

confidence: 90%

“…6 Previous studies characterizing the biofilm microbial community in MBRs (Lim et al, 2004;Choi et al, 2006;Jinhua et al, 2006;Zhang et al, 2006;Miura et al, 2007;Huang et al, 2008;Fontanos et al, 2010;Lim et al, 2012;Piasecka et al, 2012;Lee et al, 2014, showed that the biofilm microbial community was distinct from the AS community. Despite these numerous studies, we still know little of the bacteria that form biofilms on membrane surfaces of MBRs.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…While most previous studies have characterized the microbial communities in mature biofilms (Jinhua et al, 2006;Miura et al, 2007;Huang et al, 2008;Fontanos et al, 2010;Lee et al, 2014;Jo et al, 2016), some researchers claim that characterizing the early colonizers on membrane surfaces might help develop better biofouling control strategies; yet, few studies have addressed these early colonizers (Choi et al, 2006;Zhang et al, 2006;Lim et al, 2012;Piasecka et al, 2012). Also, studies characterizing both the early colonizers and mature biofilm communities in MBRs are lacking.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome

et al. 2017

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Finding efficient biofouling control strategies requires a better understanding of the microbial ecology of membrane biofilm communities in membrane bioreactors (MBRs). Studies that characterized the membrane biofilm communities in lab-and pilot-scale MBRs are numerous, yet similar studies in full-scale MBRs are limited. Also, most of these studies have characterized the mature biofilm communities with very few studies addressing early biofilm communities. In this study, five full-scale MBRs located in Seattle (Washington, U.S.A.) were selected to address two questions concerning membrane biofilm communities (early and mature): (i) Is the assembly of biofilm communities (early and mature) the result of random immigration of species from the source community (i.e. activated sludge)? and (ii) Is there a core membrane biofilm community in full-scale MBRs? Membrane biofilm (early and mature) and activated sludge (AS) samples were collected from the five MBRs, and 16S rRNA gene sequencing was applied to investigate the bacterial communities of AS and membrane biofilms (early and mature). Alpha and beta diversity measures revealed clear differences in the bacterial community structure between the AS and biofilm (early and mature) samples in the five full-scale MBRs. These differences were mainly due to the presence of large number of unique but rare operational taxonomic units (∼13% of total reads in each MBR) in each sample. In contrast, a high percentage (∼87% of total reads in each MBR) of sequence reads was shared between AS and biofilm samples in each MBR, and these shared sequence reads mainly belong to the dominant taxa in these samples. Despite the large fraction of shared sequence reads between AS and biofilm samples, simulated biofilm communities from random sampling of the respective AS community revealed that biofilm communities differed significantly from the random assemblages (P < 0.001 for each MBR), indicating that the biofilm communities (early and mature) are unlikely to represent a random sample of the AS community. In addition to the presence of unique operational taxonomic units in each biofilm sample (early or mature), comparative analysis of operational taxonomic units and genera revealed the presence of a core biofilm community in the five full-scale MBRs. These findings provided insight into the membrane biofilm communities in full-scale MBRs. More comparative studies are needed in the future to elucidate the factors shaping the core and unique biofilm communities in full-scale MBRs.

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Section: Accepted Manuscriptsupporting

confidence: 90%

Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

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Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome

et al. 2017

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“…). The Opitutus genera have been also detected in biofilms converting nitrogenous metabolites into atmospheric nitrogen (Lee, Hong, Kim, Hong & Park ), and can use almost all of the monosaccharide exudates produced by Navicula sp. as carbon sources (Kaluzhnaya et al .…”

Section: Discussionmentioning

confidence: 99%

“…The species has been usually associated to paddy soil (Chin, Liesack & Janssen 2001), but recently to the marine environment also (Kaluzhnaya et al 2012). The Opitutus genera have been also detected in biofilms converting nitrogenous metabolites into atmospheric nitrogen (Lee, Hong, Kim, Hong & Park 2014), and can use almost all of the monosaccharide exudates produced by Navicula sp. as carbon sources (Kaluzhnaya et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Bacterial diversity studied by next‐generation sequencing in a mature phototrophic Navicula sp‐based biofilm promoted into a shrimp culture system

et al. 2016

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The bacterial diversity of a phototrophic biofilm used as nutrient‐recycler into a shrimp aquaculture system and as direct food‐source for shrimp was studied by next‐generation sequencing, considering the 16S rDNA and metagenomics sequence classification using exact alignments. Biofilm was promoted by the modification of cabon:nitrogen ratio and by the addition of the diatom Navicula sp. as promoter. Results revealed a wide diversity of bacteria thriving into the biofilm; most of the bacteria detected in the biofilm belonged to the Proteobacteria (47%), Chlamydiae/Verrucomicrobia (11%), Bacteriodetes (8%), Planctomycetes (5%) Phylum. Species involved in the decomposition of organic matter, nitrogenous‐ and sulfurous metabolites were detected; moreover, filamentous species known as biomass‐bulking enhancers and producers of adhesin‐like compounds were also detected. Surprising results were also obtained by detecting both, strictly anaerobic and aerobic bacteria involved into the metabolism of nitrogenous compounds. Other species not belonging to the marine environment were also detected, but their role is unclear. Finally, the detection of most of these species may constitute a first case report for a phototrophic biofilm. The results suggest an important role of bacteria in this type of biofilm and a complex microbial‐interaction network.

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A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time

Noble

Park

Olson

et al. 2016

Appl Microbiol Biotechnol

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Aeration diffusers in wastewater treatment plants generate air bubbles that promote mixing, distribution of dissolved oxygen, and microbial processing of dissolved and suspended matter in bulk solution. Biofouling of diffusers represents a significant problem to wastewater treatment plants because biofilms decrease oxygen transfer efficiency and increase backpressure on the blower. To better understand biofouling, we conducted a pilot study to survey the bacterial community composition and function of biofilms on different diffuser substrates and compare them to those in the bulk solution. DNA was extracted from the surface of ethylene-propylene-diene monomer (EPDM), polyurethane, and silicone diffusers operated for 15 months in a municipal treatment plant and sampled at 3 and 9 months. The bacterial community composition and function of the biofilms and bulk solution were determined by amplifying the 16S rRNA genes and pyrosequencing the amplicons and raw metagenomic DNA. The ordination plots and dendrograms of the 16S rRNA and functional genes showed that while the bacterial community composition and function of the bulk solution was independent of sampling time, the composition and function of the biofilms differed by diffuser type and testing time. For the EPDM and silicone diffusers, the biofilm communities were more similar in composition to the bulk solution at 3 months than 9 months. In contrast, the bacteria on the polyurethane diffusers were more dissimilar to the bulk solution at 3 months than 9 months. Taken together, the survey showed that the community composition and function of bacterial biofilms depend on the diffuser substrate and testing time, which warrants further elucidation.

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Comparison of bacterial communities of biofilms formed on different membrane surfaces

Cited by 19 publications

References 13 publications

Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome

Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome

Bacterial diversity studied by next‐generation sequencing in a mature phototrophic Navicula sp‐based biofilm promoted into a shrimp culture system

A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time

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